Development of a highly sensitive inhibition immunoassay for microcystin-LR

Compact quantitative optic fiber-based immunoarray biosensor for rapid detection of small analytes

“…Although the same immunoarray sensor was used for determining the TNT and MC-LR, their LODs were very different. The LOD for MC-LR was less than the guideline value of 1 g/L in drinking water proposed by the World Health Organization (WHO, 2004), and was highly comparable to most of the other immunoassays demonstrated for MC-LR (Lindner et al, 2004;Sheng et al, 2006;Campàs and Marty, 2007). However, the LOD for TNT was a little high.…”

Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples

“…When the antibody concentration is 0.28 g/mL, the LOD of the sensor is less than the requirements for determination of MC-LR in drinking water as imposed by WHO (WHO, 2004). The high sensitivity of TOPA, which is highly comparable to most of the other immunoassays demonstrated for MC-LR (Lindner et al, 2004;Sheng et al, 2006;Campàs and Marty, 2007), is possibly due to the surface chemistry of the MC-LR-OVA conjugates on the probe surface that maintained the activity of the immobilized MC-LR towards MC-LR-MAb, as well as diminished the non-specific adsorption. Meanwhile, the present TOPA system also manifests its added advantages, such as the regeneration of the sensor without compromising immunoreactivity for multiple analyses.…”

“…Fast quantitative detection of these toxins is indeed essential to ensure water quality, and therefore, human health. Currently, several detection methods are in use, including high-performance liquid chromatography (HPLC) (Rivasseau et al, 1998), invertebrate bioassays (Campbell et al, 1994), protein phosphatase inhibition assays (PPIA) (Ward et al, 1997;Rivasseau et al, 1999) and immunoassays (Lindner et al, 2004;Sheng et al, 2006;Campàs and Marty, 2007) and so on. The HPLC, while being more precise than the other methods and allowing the identification of individual variants, nonetheless requires expensive equipment, complex procedures, long analysis times, and trained personnel (Tsuji et al, 1994).…”

Immunoassays and Biosensors for the Detection of Cyanobacterial Toxins in Water

“…This antibody was used in quite a few applications [52,71–98]. Mikhailov et al presented highly selective, monoclonal antibodies against nodularin [99] with negligible reactivity against microcystins.…”