Development of a HPTLC method to profile the phytochemicals in Allanblackia parviflora (tallow tree) kernel and seed cakes

Sefah, Wilfred; Sefah, Lydia; Ofori, Hayford

doi:10.1007/s00764-019-00009-9

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…Self-Assembly of DNA Nanoflowers Using RCR.-DNA nanoflowers were prepared according to our previous reports. 15 A phosphorylated linear template (0.6 μM) and a primer (1.2 μM) were mixed in DNA ligation buffer (50 mM Tris-HCl, 1 mM MgCl 2 , 0.1 mM ATP, and 1 mM dithiothreitol) and annealed at 95 °C for 5 min, followed by gradual cooling to room temperature over 1 h. The annealed product was incubated with E. coli DNA ligase (10 U/μL; Takara Bio, Dalian, China) at 16 °C for 4 h. For RCR, the circularized template (0.3 μM, unless otherwise denoted) was incubated with Φ29 DNA polymerase (2 U/μL), dNTP (2 mM), and BSA (1×) in buffer solution (50 mM Tris-HCl, 10 mM (NH 4 ) 2 SO 4 , 10 mM MgCl 2 , and 4 mM dithiothreitol) (New England Biolabs, Ipswich, MA) at 30 °C. Sgc8-NFs 3h and Sgc8-NFs were formed after RCR reaction for 3 and 24 h, respectively.…”

Section: Synthesis Of Ferrocenementioning

confidence: 99%

Engineering of Bioinspired, Size-Controllable, Self-Degradable Cancer-Targeting DNA Nanoflowers via the Incorporation of an Artificial Sandwich Base

et al. 2019

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In this article, we used an artificial DNA base to manipulate the formation of DNA nanoflowers (NFs) to easily control their sizes and functionalities. Nanoflowers have been reported as the noncanonical self-assembly of multifunctional DNA nanostructures, assembled from long DNA building blocks generated by rolling circle replication (RCR). They could be incorporated with myriad functional moieties. However, the efficacy of these DNA NFs as potential nanocarriers delivering cargo in biomedicine is limited by the bioavailability and therapeutic efficacy of their cargo. Here we report the incorporation of metal-containing artificial analogues into DNA strands to control the size and the functions of NFs. We have engineered bioinspired, size-controllable, self-degradable cancer-targeting DNA nanoflowers (Sgc8-NFs-Fc) via the incorporation of an artificial sandwich base. More specifically, the introduction of a ferrocene base not only resulted in the size controllability of Sgc8-NFs-Fc from 1000 to 50 nm but also endowed Sgc8-NFs-Fc with self-degradability in the presence of H 2 O 2 via Fenton's reaction. In vitro experiments confirmed that Sgc8-NFs-Fc/Dox could be selectively taken up by protein tyrosine kinase 7 (PTK7)-positive cancer cells and subsequently cleaved via Fenton's reaction, resulting in rapid release kinetics, nuclear accumulation, and enhanced cytotoxicity of their cargo. In vivo experiments further *

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Section: Synthesis Of Ferrocenementioning

confidence: 99%

Engineering of Bioinspired, Size-Controllable, Self-Degradable Cancer-Targeting DNA Nanoflowers via the Incorporation of an Artificial Sandwich Base

et al. 2019

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“…Vegetable oil is obtained from Allanblackia seed, while the bitter seedcake can be used as an animal feed (Sefah et al, 2020;Sefah et al, 2019). Traditionally, oils extracted from seeds have been used locally for cooking, preparing medicines and making soap at a subsistence level.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Characterization of Vegetable Oil from 20 Accessions of Allanblackia parviflora Seeds in Ghana

Ofori¹,

Korang²,

Asomaning³

et al. 2023

Ag. Food. Sc. J Gh

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The benefits derived from the forest estate in Ghana include Non-Timber Forest Products (NTFPs). NTFPs such as vegetable oil produced from seed of forest trees have been used for centuries. The oil from the seed kernel of Allanblackia parviflora (Allanblackia) is investigated for differences in the yield and quality between accessions. Vegetable oil from twenty (20) accessions of Allanblackia were extracted and characterized. The oil yields from the different accessions were all above 60% and no significant differences were detected amongst the accessions. The iodine value was very low indicating a very saturated oil whilst the refractive index, specific gravity and acid values were all within the range of most edible oils. The vegetable oil contains over 60% saturated fatty acids in the form of stearic acid (C18:0) and over 30% unsaturated fatty acid in the form of oleic acid (C18:1), and this may be the reason for the oil been solid at ambient room temperature.

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“…3,6 UBPs are also being developed by the Hirao and Benner groups to expand the chemical space available to functional nucleic acids. [7][8] In addition, there are many other potential applications, ranging from information storage [9][10] to the development of novel materials. [11][12] However, these applications would all be facilitated by improved sequencing methods.…”

mentioning

confidence: 99%

“…[15][16] Signal termination during Sanger sequencing has been employed to sequence DNA, either when the cognate unnatural triphosphate is not present 17 or when it is replaced with its dideoxynucleoside analog. 18 Condition-dependent forced loss of a UBP has been used to indirectly sequence DNA containing UBPs, [7][8] but this method is labor intensive and possibly dependent on sequence context. Moreover, with all of the developed methods, determination of which unnatural nucleotide is present in a given strand is more challenging.…”

mentioning

confidence: 99%

Nanopore Sequencing of an Expanded Genetic Alphabet Reveals High-Fidelity Replication of a Predominantly Hydrophobic Unnatural Base Pair

et al. 2020

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Unnatural base pairs (UBPs) have been developed and used for a variety of in vitro applications, as well as for the engineering of semi-synthetic organisms (SSOs) that store and retrieve increased information. However, these applications are limited by the availability of methods to rapidly and accurately determine the sequence of unnatural DNA. Here, we report the development and application of the MspA nanopore to sequence DNA containing the dTPT3-dNaM UBP. Analysis of two sequence contexts reveals that DNA containing the UBP is replicated with an efficiency and fidelity similar to that of natural DNA and sufficient for use as the basis of an SSO that produces proteins with non-canonical amino acids.

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Development of a HPTLC method to profile the phytochemicals in Allanblackia parviflora (tallow tree) kernel and seed cakes

Cited by 4 publications

References 19 publications

Engineering of Bioinspired, Size-Controllable, Self-Degradable Cancer-Targeting DNA Nanoflowers via the Incorporation of an Artificial Sandwich Base

Engineering of Bioinspired, Size-Controllable, Self-Degradable Cancer-Targeting DNA Nanoflowers via the Incorporation of an Artificial Sandwich Base

Extraction and Characterization of Vegetable Oil from 20 Accessions of Allanblackia parviflora Seeds in Ghana

Nanopore Sequencing of an Expanded Genetic Alphabet Reveals High-Fidelity Replication of a Predominantly Hydrophobic Unnatural Base Pair

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