Development of a human serum albumin structure-based fluorescent probe for bioimaging in living cells

A novel FA1-targeting fluorescent probe for specific discrimination and identification of human serum albumin from bovine serum albumin

“…[15][16][17][18] Most of these probes are designed to bind into the representative drug sites (DS1 and DS2) in HSA through host-guest interactions to change the emission signals utilizing mechanisms such as intramolecular charge transfer (ICT), aggregation-induced fluorescence quenching (ACQ), and twisted intramolecular charge transfer (TICT). [19][20][21] However, owing to the structural similarity of HSA and BSA and their similar responsiveness, it is difficult for these fluorescent probes relying on a single response mechanism to distinguish HSA from BSA. For the few reported probes that can distinguish HSA from BSA, they mainly rely on the difference in the intensity of their interaction with the two serum proteins to induce differences in fluorescence signal intensity for differentiation.…”

A novel fluorescence probe for the detection of water content in organic solvents and the distinction between deuterated and nondeuterated reagents

“…[49,50] Based on the distorted intramolecular charge transfer (TICT) mechanism, the free rotation of the probe is limited by the HSA pocket, resulting in the fluorescence being 'switched on', therefore allowing the identification and detection of HSA by the probe. [51,52] In this work, a novel fluorescent probe (probe 1) was designed and synthesized with an amino group as an electron-donor group (D), a furan as a π-bridge, and an α, β-unsaturated ketone-naphthalene as an electron-acceptor moiety (A), therefore forming a typical DÀπÀA fluorescent probe. Probe 1 exhibited prominent polarity sensitivity with ICT characteristics.…”