Development of a liquid chromatography–electrospray ionization–tandem mass spectrometry method for the simultaneous analysis of intact glucosinolates and isothiocyanates in Brassicaceae seeds and functional foods

Franco, Placido; Spinozzi, Silvia; Pagnotta, Eleonora; Lazzeri, Luigi; Ugolini, Luisa; Camborata, Cecilia; Roda, Aldo

doi:10.1016/j.chroma.2015.09.001

Cited by 49 publications

(43 citation statements)

References 32 publications

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“…Furthermore, the ITC profile of each cruciferous species varies according to the cultivar or variety considered and also according to the plant tissue found [27,28]. For example, in rocket leaves, the main ITC generally detected corresponds to sativin [29], but in rocket seeds, the main ITC detected have been erucin and sulforaphane (SFN) [30]. Accordingly, in radish seeds and sprouts, the main ITC detected was sulforaphene according to several reports [13,27,28,31] (Figure 2), but in radish roots, the chief ITC have been raphasatin and sulforaphene [27,32].…”

Section: Organosulfur Compoundsmentioning

confidence: 99%

Functional Ingredients From Brassicaceae Species: Overview and Perspectives

Ramírez

Abellán-Victorio

Beretta

et al. 2020

IJMS

104

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Brassicaceae vegetables are important crops consumed worldwide due to their unique flavor, and for their broadly recognized functional properties, which are directly related to their phytochemical composition. Isothiocyanates (ITC) are the most characteristic compounds, considered responsible for their pungent taste. Besides ITC, these vegetables are also rich in carotenoids, phenolics, minerals, and vitamins. Consequently, Brassica’s phytochemical profile makes them an ideal natural source for improving the nutritional quality of manufactured foods. In this sense, the inclusion of functional ingredients into food matrices are of growing interest. In the present work, Brassicaceae ingredients, functionality, and future perspectives are reviewed.

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Section: Organosulfur Compoundsmentioning

confidence: 99%

Functional Ingredients From Brassicaceae Species: Overview and Perspectives

Ramírez

Abellán-Victorio

Beretta

et al. 2020

IJMS

104

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“…These metabolic compounds play a vital role in the physiology and protection of plants against several environmental stresses [ 20 ]. More than 200 different types of GSLs have been studied, and 30 of them are identified in Brassica crops [ 20 , 21 ]. GSLs are major secondary metabolites synthesized by plants.…”

Section: Plant-derived Sulfur Compounds—gsls and Oscsmentioning

confidence: 99%

Health Benefits of Plant-Derived Sulfur Compounds, Glucosinolates, and Organosulfur Compounds

et al. 2020

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The broad spectrum of the mechanism of action of immune-boosting natural compounds as well as the complex nature of the food matrices make researching the health benefits of various food products a complicated task. Moreover, many routes are involved in the action of most natural compounds that lead to the inhibition of chronic inflammation, which results in a decrease in the ability to remove a pathogen asymptomatically and is connected to various pathological events, such as cancer. A number of cancers have been associated with inflammatory processes. The current review strives to answer the question of whether plant-derived sulfur compounds could be beneficial in cancer prevention and therapy. This review focuses on the two main sources of natural sulfur compounds: alliaceous and cruciferous vegetables. Through the presentation of scientific data which deal with the study of the chosen compounds in cancer (cell lines, animal models, and human studies), the discussion of food processing’s influence on immune-boosting food content is presented. Additionally, it is demonstrated that there is still a need to precisely demonstrate the bioavailability of sulfur-containing compounds from various types of functional food, since the inappropriate preparation of vegetables can significantly reduce the content of beneficial sulfur compounds. Additionally, there is an urgent need to carry out more epidemiological studies to reveal the benefits of several natural compounds in cancer prevention and therapy.

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“…In 2014, a novel HPLC approach for analyzing reaction kinetics was described and validated against the UV–Vis method; key advantages of the HPLC approach were the ability to chromatographically separate and simultaneously track analytes with diverse polarity/solubility profiles, such as 1 and 2 [19]. This methodology has more recently been expanded to LC–MS systems to aid in the evaluation of samples from complex mixtures and has broader implications as an analytical approach [24]. …”

Section: Introductionmentioning

confidence: 99%

HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation

Klingaman

Wagner

Brown

et al. 2017

Analytical Biochemistry

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Glucosinolates are plant secondary metabolites abundant in Brassica vegetables that are substrates for the enzyme myrosinase, a thioglucoside hydrolase. Enzyme-mediated hydrolysis of glucosinolates forms several organic products, including isothiocyanates (ITCs) that have been explored for their beneficial effects in humans. Myrosinase has been shown to be tolerant of non-natural glucosinolates, such as 2,2-diphenylethyl glucosinolate, and can facilitate their conversion to non-natural ITCs, some of which are leads for drug development. An HPLC-based method capable of analyzing this transformation for non-natural systems has been described. This current study describes (1) the Michaelis–Menten characterization of 2,2-diphenyethyl glucosinolate and (2) a parallel evaluation of this analogue and the natural analogue glucotropaeolin to evaluate effects of pH and temperature on rates of hydrolysis and product(s) formed. Methods described in this study provide the ability to simultaneously and independently analyze the kinetics of multiple reaction components. An unintended outcome of this work was the development of a modified Lambert W(x) which includes a parameter to account for the thermal denaturation of enzyme. The results of this study demonstrate that the action of Sinapis alba myrosinase on natural and non-natural glucosinolates is consistent under the explored range of experimental conditions and in relation to previous accounts.

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Development of a liquid chromatography–electrospray ionization–tandem mass spectrometry method for the simultaneous analysis of intact glucosinolates and isothiocyanates in Brassicaceae seeds and functional foods

Cited by 49 publications

References 32 publications

Functional Ingredients From Brassicaceae Species: Overview and Perspectives

Functional Ingredients From Brassicaceae Species: Overview and Perspectives

Health Benefits of Plant-Derived Sulfur Compounds, Glucosinolates, and Organosulfur Compounds

HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation

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