Development of a liquid chromatography–tandem mass spectrometry with pressurized liquid extraction method for the determination of benzimidazole residues in edible tissues

Determination of benzimidazole residues and their metabolites in raw milk using high performance liquid chromatography-diode array detection

“…No interfering peaks were observed for the retention time for benzimidazoles, except for the retention times for the triclabendazole metabolites and FEBZ-SO 2 (Fig. 1) where an interfering peak of negligible size, relative to MRL concentration, were observed.…”

“…Mean recoveries (n = 6) of the analytes, determined in six separate assays, were between 60 % and 100 % (Table 1). Lower mean recoveries of approximately 30 % were obtained for KTCB and TCB-SO 2 , higher mean recoveries of approximately 120 % were obtained for ABZ-SO 2 2007) indicated that TCBZ metabolites are in a neutral state at a lower pH than other benzimidazoles and that this can have an influence on the repeatability of the extraction of these compounds. In a similar study using liquid-liquid extraction, sample preparation with acetonitrile, with an additional solid-phase sample extraction step procedure, followed by quantification Table 2.…”

A Liquid Chromatography – Tandem Mass Spectrometry Approach for the Identification of Mebendazole Residue in Pork, Chicken, and Horse

“…In this context, Zue et al [18] have reported that the responses of methanol-water + 0.1% formic acid are greater than those obtained when acetonitrile-water + 0.1% formic acid is used to provide protons in positive mode of LC-MS/MS aided protonation of benzimidazoles. Many authors have used ammonium formate and formic acid in mobile phase to increase ionization and achieve maximum sensitivity [10, 11, 15, 17–19]. In this respect, three reagents (0.1% formic acid, 10 mM ammonium formate, and 20 mM ammonium formate) with methanol were tested in this study (Fig 3).…”

“…However, these detectors have low sensitivity and specificity. To enhance the detection level, the combination of liquid chromatography and mass spectrometric detection for the determination of MEB residues has become a popular analytical technique for sensitive and selective detection of MEB residue in complex biological matrices [1, 10, 11]. In addition, liquid-liquid extraction (LLE) with additional clean-up and SPE are often used for sample preparation in food-producing animals.…”

Development of an Improved HPLC-PDA Method for the Determination of Tolfenamic Acid in Meat and Milk

“…In addition, LLE with additional clean-up and solid phase extraction (SPE) are often used for sample preparation in food-producing animals [18]. The SPE is generally applied to clean animal products but does not provide sufficient extraction efficiency [19]. To sum up, methods capable for simultaneous determination of TA in cattle, pork, and milk by HPLC-PDA with LLE have not been developed.…”