Development of a membrane of poly (L-co-D,L lactic acid-co-trimethylene carbonate) with aloe vera: An alternative biomaterial designed to improve skin healing

Abstract

The search for new therapies and drugs that act as topical agents to relieve pain and control the infectious processes in burns always attracted interest in clinical trials. As an alternative to synthetic drugs, the use of natural extracts is useful in the development of new strategies and formulations for improving the life quality. The aim of this study was to develop a wound dressing using Poly(L-co-D,L lactic acid-co-TMC) (PLDLA-co-TMC) containing aloe vera (AV). This natural plant extract is known for its modulatory effects under healing process. The membrane of PLDLA-co-TMC+aloe vera was prepared at different concentrations of AV (5, 10, 15 and 50%). The FTIR showed no change in the PLDLA-co-TMC spectrum after AV addition, while the swelling test showed changes only in PLDLA-co-TMC+AV at 50%. The wettability measurements showed decrease in the contact angle in all samples after the AV addition in the polymer, while the AV release test showed that PLDLA-co-TMC+50%AV sample has higher AV release rate than the sample with other AV concentrations. The SEM analysis showed that AV was homogeneously distributed at 5% only. Tensile tests demonstrated an increase in the Young's modulus and a reduction in the elongation till rupture of the PLDLA-co-TMC after the addition of AV. Biocompatibility in vitro evaluation with fibroblast cells seeded in the membranes of PLDLA-co-TMC+AV showed that the cells were able to adhere, proliferate and maintain mitochondrial activity in all AV concentrations tested. Due to the known skin medicinal properties attributed to AV and the results here obtained, we suggest that after in vivo trials, the PLDLA-co-TMC+AV should be a promising biomaterial for application as a device for skin curative and healing agent.

Poly(L-co-D,L lactic acid-co-Trimethylene Carbonate) 3D printed scaffold cultivated with mesenchymal stem cells directed to bone reconstruction: In vitro and in vivo studies

“…It was developed by our research group as a promising material, due capacity of PLDLA to its biological reabsorption abilities and biocompatibility. Previous studies in recent literature related to PLDLA-TMC based-materials in skin tissue regeneration 7,8 and tendon 9 demonstrated that the terpolymer is versatile in a wide range of medical applications, as well as presenting biocompatibility characteristics. Furthermore, the TMC shows flexibility and degradation rates consistent with the applications designed to bone tissue engineering.…”

Alternative Cutaneous Substitutes Based on Poly(l-co-d,l-lactic acid-co-trimethylene carbonate) with Schinus terebinthifolius Raddi Extract Designed for Skin Healing

“…Thus, the ideal concentration used is mandatory to determine how inductive the extract could be, i.e., whether it presents bioactive properties. Recently, it was shown that PLDLA-TMC containing Aloe vera could induce even at higher concentrations a strong increase in cell growth . Since the surface topography and the substances released can influence directly cell adhesion, no washing steps were performed for LCSM live-imaging observations.…”

“…Recently, it was shown that PLDLA-TMC containing Aloe vera could induce even at higher concentrations a strong increase in cell growth. 28 Since the surface topography and the substances released can influence directly cell adhesion, no washing steps were performed for LCSM live-imaging observations. In this way, as observed in Figure 8, the fibroblasts adhered to all PLDLA-TMC containing S.T.R.…”

In-vivo histocompatibility and osteogenic potential of biodegradable PLDLA composites containing silica-based bioactive glass fiber

“…21 Therefore, PLDLA would not cause significant foreign body reaction in the long term. Ignatius and Claes, 27 Esposito et al, 28 and Komatsu et al 29 reported that PLDLA and its composites had good histocompatibility and the potential for tissue engineering and craniomaxillofacial surgery. And SR-PLDLA (DL:L, 70:30) plates and screws have been used as self-reinforced bioabsorbable devices intended for the craniomaxillofacial skeleton or cranioplasties in children.…”