Development of a method to measure bacterial transfer from hands

Lingaas, Egil; Fagernes, Mette

doi:10.1016/j.jhin.2009.01.022

Cited by 32 publications

(33 citation statements)

References 33 publications

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“…If this limit was exceeded, all samples in the sequential‐touch trial were discarded. At this pressure, the contact area between the thumb and the glass surface was 3·6 cm 2 ; therefore, the pressure was approximately 0·2–0·3 kg cm –2 , which is consistent with values reported in previous studies (Chen et al ; Rusin et al ; Paulson ; Lingaas and Fagernes ; Julian et al ; Stals et al ; Greene et al ).…”

Section: Methodssupporting

confidence: 91%

New sequential‐touch method to determine bacterial contact transfer rate from finger to surface

Zhao

2019

J of Applied Microbiology

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Aims Infection can occur via surface touch. A new method was developed to more accurately evaluate the bacterial transfer rate from a finger to a surface. Methods and Results Staphylococcus aureus was used as the model bacteria to inoculate a thumb. Sequential touches were made between the contaminated thumb and a series of clean microscope slides. The bacteria on the glass surface were cultured in situ, and the bacterial transfer rate was evaluated by fitting the colony‐forming units (CFUs) on glass surfaces with the exponential decay function. The resident microflora on thumb were also used to validate the new method. Conclusions The average transfer rate was 12·9% for S. aureus under the baseline condition. The CFUs counted on the glass surfaces were well fitted by the exponential decay function. A set of trials with more surfaces resulted in a more accurate evaluation. No statistically significant difference was found in the estimated transfer rate between the standard strain and the resident microflora. Significance and Impact of the Study The new method significantly increases the accuracy of evaluation of the microbial transfer rate from a finger to a surface while maintaining a low workload.

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Section: Methodssupporting

confidence: 91%

New sequential‐touch method to determine bacterial contact transfer rate from finger to surface

Zhao

2019

J of Applied Microbiology

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“…Hand contamination levels are likely to vary depending on several factors, including the degree to which hands were contaminated during defecation, whether the individual washed his/her hands with water and soap before sample collection, the quality and duration of handwashing, the level of environmental contamination with feces on surfaces, and the length of time since last handwashing or defecation. Given the rapid decline in E. coli survival on skin, we assume that the contamination we detected on hands was recently acquired while at school 16,31. Our data show that handwashing materials were more frequently available following the intervention.…”

Section: Discussionmentioning

confidence: 69%

Impact of a School-Based Hygiene Promotion and Sanitation Intervention on Pupil Hand Contamination in Western Kenya: A Cluster Randomized Trial

Greene¹,

Freeman²,

Akoko³

et al. 2012

The American Society of Tropical Medicine and Hygiene

105

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Handwashing with soap effectively reduces exposure to diarrhea-causing pathogens. Interventions to improve hygiene and sanitation conditions in schools within low-income countries have gained increased attention; however, their impact on schoolchildren's exposure to fecal pathogens has not been established. Our trial examined whether a school-based water, sanitation, and hygiene intervention reduced Escherichia coli contamination on pupils' hands in western Kenya. A hygiene promotion and water treatment intervention did not reduce risk of E. coli presence (relative risk [RR] = 0.92, 95% confidence interval [CI] = 0.54–1.56); the addition of new latrines to intervention schools significantly increased risk among girls (RR = 2.63, 95% CI = 1.29–5.34), with a non-significant increase among boys (RR = 1.36, 95% CI = 0.74–2.49). Efforts to increase usage of school latrines by constructing new facilities may pose a risk to children in the absence of sufficient hygiene behavior change, daily provision of soap and water, and anal cleansing materials.

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“…The glove-juice method 3–5 is recognized as a standard for microbial sampling of hands, but not for sampling gloved hands. In the glove-juice method the participant places their hand in a sterile glove and sampling solution is added.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Two Glove-Sampling Methods to Discriminate Between Study Arms of a Hand Hygiene and Glove-Use Study

Robinson

Otieno

Johnson

et al. 2018

Infect. Control Hosp. Epidemiol.

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Development of a method to measure bacterial transfer from hands

Cited by 32 publications

References 33 publications

New sequential‐touch method to determine bacterial contact transfer rate from finger to surface

New sequential‐touch method to determine bacterial contact transfer rate from finger to surface

Impact of a School-Based Hygiene Promotion and Sanitation Intervention on Pupil Hand Contamination in Western Kenya: A Cluster Randomized Trial

Comparison of Two Glove-Sampling Methods to Discriminate Between Study Arms of a Hand Hygiene and Glove-Use Study

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