2015
DOI: 10.1002/jssc.201500440
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Development of a method using high‐performance liquid chromatographic fingerprint and multi‐ingredients quantitative analysis for the quality control of Yangxinshi Pian

Abstract: A simple and reliable method of high-performance liquid chromatography with diode array detection method was developed for fingerprint analysis and simultaneous determination of six compounds including puerarin, salvianolic acid B, berberine hydrochloride, palmatine chloride, dehydrocorydaline, and icariin in the Chinese medicine preparation Yangxinshi Pian. The separation was performed on an Agilent Eclipse XDB-C18 reserved-phase column (250 mm × 4.6mm I.D., 5 μm) using gradient elution with 50 mmol/L monopot… Show more

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Cited by 22 publications
(12 citation statements)
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“…To date, conventional RP C18 stationary phases have been the most commonly used to isolate TCM compounds [13,[15][16][17][18]. Nevertheless, these phases are unable to provide satisfactory separation, such as insufficient resolution and peak capacity.…”
Section: Introductionmentioning
confidence: 99%
“…To date, conventional RP C18 stationary phases have been the most commonly used to isolate TCM compounds [13,[15][16][17][18]. Nevertheless, these phases are unable to provide satisfactory separation, such as insufficient resolution and peak capacity.…”
Section: Introductionmentioning
confidence: 99%
“…Some publications have explored that single-wavelength HPLC fingerprint was collected to characterize the chemical constituents of TCM. For example, Wang et al [11] and Li et al [12] adopted single-wavelength to develop HPLC chromatographic fingerprint and multiingredients quantitative analysis for controlling the quality of TCM, respectively. However, this method does not provide a complete profile of the drug, neglecting chromatographic peaks that were important to characterize the properties of TCM.…”
Section: Introductionmentioning
confidence: 99%
“…and Li et al. adopted single‐wavelength to develop HPLC chromatographic fingerprint and multi‐ingredients quantitative analysis for controlling the quality of TCM, respectively. However, this method does not provide a complete profile of the drug, neglecting chromatographic peaks that were important to characterize the properties of TCM.…”
Section: Introductionmentioning
confidence: 99%
“…In recent years, various types of fingerprinting techniques have played more important roles in mediating the QC of herbal medicine. The use of fingerprint similarity analyses to identify herbal medicines or to assess herbal medicine has been an effective approach. However, this method does not evaluate the relationship between dynamic changes in the chemical compounds and the pharmacological activities of TCMs.…”
Section: Introductionmentioning
confidence: 99%