Development of a microhand using direct laser writing for indirect optical manipulation

Avci, Ebubekir; Yang, Guang‐Zhong

doi:10.1109/iros.2016.7759752

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…Our experiment uses a two-trap setup to test and quantify optical force amplification (a), with the hope that such levers could become useful tools in areas such as molecule stretching (b) and cell studies (c). [4], large, flat objects that scatter the beam [9] and complex biological cells with varied refractive indices [10].…”

Section: Introductionmentioning

confidence: 99%

Developing an Optical Microlever for Stable and Unsupported Force Amplification

Andrew

Raudsepp

Nock

et al. 2022

2022 International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS)

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Optical micromachines have the potential to improve the capabilities of optical tweezers by amplifying forces and allowing for indirect handling and probing of specimens. However, systematic design and testing of micromachine performance is still an emerging field. In this work we have designed and tested an unsupported microlever, suitable for general-purpose optical tweezer studies, that demonstrates stable trapping performance and repeatable doubling of applied forces. Stable trapping was ensured by analysing images to monitor focus shift when levers oscillated repeatedly, before the best-performing design was selected for force amplification. This study also shows that direct measurement of trap stiffness using the equipartition theorem appears to be a valid method for measuring applied forces on the spherical handles of microlevers.

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Section: Introductionmentioning

confidence: 99%

Developing an Optical Microlever for Stable and Unsupported Force Amplification

Andrew

Raudsepp

Nock

et al. 2022

2022 International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS)

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“…This actuation technique is demonstrated on both artificial and biological objects. If the objects cannot tolerate the temperature increase due to the laser beam, or if their properties do not suit optical tweezer manipulation requirements, indirect manipulation using intermediate tools controlled by laser trapping has been demonstrated [14]. Characterization of biological properties is possible using this tool.…”

Section: Introductionmentioning

confidence: 99%

1D manipulation of a micrometer size particle actuated via thermocapillary convective flows

Mallea

Bolopion

Beugnot

et al. 2017

2017 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS)

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This paper deals with the open-loop characterization of a micromanipulation system actuated by thermocapillary convective flows. Micrometric size objects placed at the air/liquid interface are actuated by heating the surface of the liquid using a laser. The heat generates a surface tension gradient at the interface which induces thermocapillary convective flows that are used to move the objects. In this paper, the performances of this approach are analyzed based on openloop experiments. Several actuation strategies are proposed and discussed. The experimental results highlight the potential of this approach since velocities up to several millimeters per second are obtained. However the precision of the positioning is not ensured by open-loop actuation, so closed-loop control will be necessary in future works. As a first step towards closedloop control, this paper proposes a model of the system. This model is based on the open-loop experimental results, but the proposed methodology can be applied to any setup that use thermocapillary convective flows for particle manipulation.

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“…To address these problems, indirect optical manipulation approaches are proposed.Indirect techniques employ intermediate micro-objects as end-effectors of optical tweezers to manipulate cells. Instead of focusing on the cells, laser beams are focused on the non-biological and biocompatible micro-objects, thereby driving the micro-objects to interact with the biological cells [29,30]. Cell manipulation is achieved under the reaction force of the micro-objects.…”

mentioning

confidence: 99%

“…Although the use of microbeads for indirect manipulation in combination with automated control strategies is well-established [33-35], their transport with microbeads is frequently unstable, and laser fields remain close to trapped cells.With the advancement of the direct laser writing technique, designable microscale mechanisms with 3D nano features were printed using the two-photon photopolymerization (2PP) method and trapped as microtools for indirect optical manipulation [36]. Various microhands, as the end-effector of optical tweezers, were proposed, and their manipulability by optical tweezers was verified [29,37]. The optical manipulation of highly task-specific micromechanisms, such as an articulated microrobot and microsyringe, as fabricated by the 2PP method, was further investigated [38][39][40].…”

mentioning

confidence: 99%

Automated Indirect Transportation of Biological Cells with Optical Tweezers and a 3D Printed Microtool

Xie

Wei

et al. 2019

Applied Sciences

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Optical tweezers are widely used for noninvasive and precise micromanipulation of living cells to understand biological processes. By focusing laser beams on cells, direct cell manipulation with optical tweezers can achieve high precision and flexibility. However, direct exposure to the laser beam can lead to negative effects on the cells. These phenomena are also known as photobleaching and photodamage. In this study, we proposed a new indirect cell micromanipulation approach combined with a robot-aided holographic optical tweezer system and 3D nano-printed microtool. The microtool was designed with a V-shaped head and an optical handle part. The V-shaped head can push and trap different sizes of cells as the microtool moves forward by optical trapping of the handle part. In this way, cell exposure to the laser beam can be effectively reduced. The microtool was fabricated with a laser direct writing system by two-photon photopolymerization. A control strategy combined with an imaging processing algorithm was introduced for automated manipulation of the microtool and cells. Experiments were performed to verify the effectiveness of our approach. First, automated microtool transportation and rotation were demonstrated with high precision. Second, indirect optical transportations of cells, with and without an obstacle, were performed to demonstrate the effectiveness of the proposed approach. Third, experiments of fluorescent cell manipulation were performed to confirm that, indicated by the photobleaching effect, indirect manipulation with the microtool could induce less laser exposure compared with direct optical manipulation. The proposed method could be useful in complex biomedical applications where precise cell manipulation and less laser exposure are required.

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Development of a microhand using direct laser writing for indirect optical manipulation

Cited by 4 publications

References 25 publications

Developing an Optical Microlever for Stable and Unsupported Force Amplification

Developing an Optical Microlever for Stable and Unsupported Force Amplification

1D manipulation of a micrometer size particle actuated via thermocapillary convective flows

Automated Indirect Transportation of Biological Cells with Optical Tweezers and a 3D Printed Microtool

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