Development of a molecularly imprinted polymer for selective extraction followed by liquid chromatographic determination of sitagliptin in rat plasma and urine

Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometric Study of Sitagliptin and its Stressed Degradation Products

“…The drug works by competitively inhibiting a protein/ enzyme, dipeptidyl peptidase 4, which results in an increased amount of active incretins (GLP-1 and GIP), reduced amount of release of glucagon and increased release of insulin. Chemically SIT is 4-oxo-4-[3-(trifl uoromethyl)-5,6-dihydro [1,2,4] triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl) butan-2-amine. Rao et al [ 1 ] developed a novel water compatible molecularly imprinted solid-phase extraction (MISPE) combined with zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) method for selective extraction and determination of sitagliptin in rat serum and urine.…”

“…Chemically SIT is 4-oxo-4-[3-(trifl uoromethyl)-5,6-dihydro [1,2,4] triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl) butan-2-amine. Rao et al [ 1 ] developed a novel water compatible molecularly imprinted solid-phase extraction (MISPE) combined with zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) method for selective extraction and determination of sitagliptin in rat serum and urine. A sensitive highperformance liquid chromatography-positive ion electrospray tandem mass spectrometric method for quantifi cation of sitagliptin in human plasma was developed by Nirogi et al [ 2 ] .…”

Biomimetic Molecular Recognition Elements for Chemical Sensing

“…As these polymers are originally imprinted in nonpolar solvents, aqueous environments can alter the selectivity of the recognition sites, hence limiting the use to nonpolar organic solvents [64]. To alleviate this problem, researchers have proposed the use of metal complexes and covalent imprinting techniques in polar solvents to promote the formation of strong bonds between the polymer and template target molecule [72,77]. Another inherent problem with MIP is the randomized placement of the template molecule through the system during the polymerization process.…”

Molecularly imprinted polymer for selective extraction of 3‐methylflavone‐8‐carboxylic acid from human urine followed by its determination using zwitterionic hydrophilic interaction liquid chromatography

“…These are often referred to as 'artificial antibodies'. Unlike antibodies, MIPs are stable to extremes of pH, organic solvents and temperatures allowing more flexibility in analytical method development [11][12][13]. MIP could be used in SPE in two different modes: (i) conventional mode where the MIP is packed into cartridges and (ii) batch mode where the MIP is incubated with the sample [14][15][16][17][18][19][20].…”

“…MIP could be used in SPE in two different modes: (i) conventional mode where the MIP is packed into cartridges and (ii) batch mode where the MIP is incubated with the sample [14][15][16][17][18][19][20]. MIP-based SPE is an efficient tool for sample clean-up due to high selectivity of the sorbent [21,22].…”