Molecularly imprinted polymer for selective extraction of 3‐methylflavone‐8‐carboxylic acid from human urine followed by its determination using zwitterionic hydrophilic interaction liquid chromatography

Rao, R. Nageswara; Maurya, Pawan K.; Kuntamukkala, Ramakrishna; Vitthal, Wani D.; Talluri, M.V.N. Kumar

doi:10.1002/jssc.201100560

Cited by 10 publications

(4 citation statements)

References 24 publications

(24 reference statements)

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“…Moreover, 3methylflavone-8-carboxylic acid is stated as one of the official impurities of FLV in the British Pharmacopoeia (BP) [6] and the United States Pharmacopeia (USP) [7]. For the determination of MFC, several analytical approaches have been devised, such as HPLC [6][7][8][9][10][11] and capillary electrophoresis [12] methods.…”

Section: Introductionmentioning

confidence: 99%

Liquid Contact Ion Selective Electrode Green Method for the Determination of Flavoxate HCl Main Active Metabolite in Pure Powder and Human Urine with Establishment of Urinary Excretion Pattern

et al. 2022

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The main aim of this work is to develop the first liquid contact ion selective electrode potentiometric method for determination of flavoxate HCl main metabolite; 3-methylflavone-8-carboxylic acid (MFC). The method can be used for studying flavoxate HCl bioavailability through tracing its active metabolite; MFC, excreted in human urine. The metabolite was determined in pure powder and in human urine with establishment of urinary excretion pattern without any sample extraction or pretreatment procedures. The suggested sensor's performance was assessed in accordance with IUPAC guidelines. According to the Bioanalytical Method Validation Guidance for Industry, the suggested method was validated where good results were obtained. The proposed method is an excellent green eco-friendly analytical method.

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Section: Introductionmentioning

confidence: 99%

Liquid Contact Ion Selective Electrode Green Method for the Determination of Flavoxate HCl Main Active Metabolite in Pure Powder and Human Urine with Establishment of Urinary Excretion Pattern

et al. 2022

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“…A literature review revealed that many analytical methods such as direct [11,12,13] and derivative [14] ultraviolet-visible (UV-Vis) spectrophotometric, spectrofluorometric [15,16], high-performance liquid chromatography (HPLC) [17,18,19,20,21], ultra-performance liquid chromatography (UPLC) [22], liquid chromatography (LC)-mass spectrometry (MS) [23,24], and capillary zone electrophoresis [25,26] methods for the determination of MOX alone and in combination with other drugs in different formulations and in different biological samples. Bibliography data showed that UV-Vis spectrophotometric [27], HPLC [28,29,30,31,32,33,34], electroanalytical [35], and capillary electrophoresis methods [36] were reported for the determination of FLX alone or in combination with other drugs. Only one reversed-phase (RP)-HPLC method has been reported for the concurrent estimation of MOX and FLX in formulation [37].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations

Attimarad

Chohan

Balgoname

2019

IJERPH

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Simple, fast, and precise reversed-phase (RP)-high-performance liquid chromatography (HPLC) and two ecofriendly spectrophotometric methods were established and validated for the simultaneous determination of moxifloxacin HCl (MOX) and flavoxate HCl (FLX) in formulations. Chromatographic methods involve the separation of two analytes using an Agilent Zorbax SB C18 HPLC column (150 mm × 4.6 mm; 5 µm) and a mobile phase consisting of phosphate buffer (50 mM; pH 5): methanol: acetonitrile in a proportion of 50:20:30 v/v, respectively. Valsartan was used as an internal standard. Analytes were monitored by measuring the absorbance of elute at 299 nm for MOX and 250 nm for FLX and valsartan. Two environmentally friendly spectrophotometric (first derivative and ratio first derivative) methods were also developed using water as a solvent. For the derivative spectrophotometric determination of MOX and FLX, a zero-crossing technique was adopted. The wavelengths selected for MOX and FLX were −304.0 nm and −331.8 nm for the first derivative spectrophotometric method and 358.4 nm and −334.1 nm for the ratio first-derivative spectrophotometric method, respectively. All methods were successfully validated, as per the International Conference on Harmonization(ICH) guidelines, and all parameters were well within acceptable ranges. The proposed analytical methods were successfully utilized for the simultaneous estimation of MOX and FLX in formulations.

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“…21,22 Also, some HPLC methods have focused on the analysis of the active metabolite of FLV, (3-methylavone-8-carboxylic acid), in human urine. 23,24 Regarding the FLV/OFL pharmaceutical mixture, a literature review revealed few methods for the simultaneous assay of the two drugs. These methods include second derivative and absorption ratio spectrophotometric methods for the simultaneous determination of the two drugs in tablets, 25 and an HPLC method for their determination in spiked human plasma employing protein precipitation pretreatment and a gradient elution mode.…”

Section: Introductionmentioning

confidence: 99%

Analysis of ofloxacin and flavoxate HCl either individually or in combination via a green chromatographic approach with a pharmacokinetic study of ofloxacin in biological samples

2015

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New sensitive and rapid micellar liquid chromatographic method was developed for determination of ofloxacin (OFL) and flavoxate hydrochloride (FLV) in different pharmaceutical formulations in addition to a pharmacokinetic study of OFL in human plasma and urine. The analyses were carried out on BDS Hypersil phenyl column (4.6 × 250 mm, 5 µm particle size) using a micellar mobile phase consisting of 0.15 M SDS, 15% n-propanol, 0.3% triethylamine and 0.02 M orthophosphoric acid (pH 2.5) with UV detection at 325 nm. The proposed method was found to be rectilinear over the concentration range of 2.0-40.0 µg mL -1 for the two drugs with lower detection limits of 0.16 and 0.32µg mL -1 for OFL and FLV, respectively. The proposed method was applied for determination of OFL and FLV in their single ingredient and co-formulated dosage forms with good percentage recoveries (99.86-99.98 %) and small standard deviation values (± 0.18 -1.26). The results of the proposed method were statistically compared with those obtained by the comparison methods revealing no significant differences in the performance of the two methods regarding accuracy and precision. To achieve high sensitivity that allows pharmacokinetic study of OFL, fluorescence detection at 290/485 nm was employed. A linear relationship was achieved over the concentration ranges of 0.01-0.10 and 0.02-0.20 µg mL -1 with mean percentage recoveries of 98.63 ± 9.84 and 100.63 ± 3.15 % for OFL in human plasma and urine, respectively.

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Molecularly imprinted polymer for selective extraction of 3‐methylflavone‐8‐carboxylic acid from human urine followed by its determination using zwitterionic hydrophilic interaction liquid chromatography

Cited by 10 publications

References 24 publications

Liquid Contact Ion Selective Electrode Green Method for the Determination of Flavoxate HCl Main Active Metabolite in Pure Powder and Human Urine with Establishment of Urinary Excretion Pattern

Liquid Contact Ion Selective Electrode Green Method for the Determination of Flavoxate HCl Main Active Metabolite in Pure Powder and Human Urine with Establishment of Urinary Excretion Pattern

Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations

Analysis of ofloxacin and flavoxate HCl either individually or in combination via a green chromatographic approach with a pharmacokinetic study of ofloxacin in biological samples

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