Analysis of ofloxacin and flavoxate HCl either individually or in combination via a green chromatographic approach with a pharmacokinetic study of ofloxacin in biological samples

Abstract

New sensitive and rapid micellar liquid chromatographic method was developed for determination of ofloxacin (OFL) and flavoxate hydrochloride (FLV) in different pharmaceutical formulations in addition to a pharmacokinetic study of OFL in human plasma and urine. The analyses were carried out on BDS Hypersil phenyl column (4.6 × 250 mm, 5 µm particle size) using a micellar mobile phase consisting of 0.15 M SDS, 15% n-propanol, 0.3% triethylamine and 0.02 M orthophosphoric acid (pH 2.5) with UV detection at 325 nm. The proposed method was found to be rectilinear over the concentration range of 2.0-40.0 µg mL -1 for the two drugs with lower detection limits of 0.16 and 0.32µg mL -1 for OFL and FLV, respectively. The proposed method was applied for determination of OFL and FLV in their single ingredient and co-formulated dosage forms with good percentage recoveries (99.86-99.98 %) and small standard deviation values (± 0.18 -1.26). The results of the proposed method were statistically compared with those obtained by the comparison methods revealing no significant differences in the performance of the two methods regarding accuracy and precision. To achieve high sensitivity that allows pharmacokinetic study of OFL, fluorescence detection at 290/485 nm was employed. A linear relationship was achieved over the concentration ranges of 0.01-0.10 and 0.02-0.20 µg mL -1 with mean percentage recoveries of 98.63 ± 9.84 and 100.63 ± 3.15 % for OFL in human plasma and urine, respectively.

Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis

“…Table 2 lists the different, recent applications of MLC for analysis of drugs in both pharmaceutical and biological matrices [ 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 ]. Although most of these methods used SDS as a surfactant, some of them also used Brij-35 [ 92 , 93 ], cetyltrimethylammonium bromide (CTAB) [ 94 ], or Tween-20 [ 95 ].…”

Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations

“…A literature review revealed that many analytical methods such as direct [11,12,13] and derivative [14] ultraviolet-visible (UV-Vis) spectrophotometric, spectrofluorometric [15,16], high-performance liquid chromatography (HPLC) [17,18,19,20,21], ultra-performance liquid chromatography (UPLC) [22], liquid chromatography (LC)-mass spectrometry (MS) [23,24], and capillary zone electrophoresis [25,26] methods for the determination of MOX alone and in combination with other drugs in different formulations and in different biological samples. Bibliography data showed that UV-Vis spectrophotometric [27], HPLC [28,29,30,31,32,33,34], electroanalytical [35], and capillary electrophoresis methods [36] were reported for the determination of FLX alone or in combination with other drugs. Only one reversed-phase (RP)-HPLC method has been reported for the concurrent estimation of MOX and FLX in formulation [37].…”