Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations

Attimarad, Mahesh; Chohan, Muhammad Shahzad; Balgoname, Abdulmalek Ahmed

doi:10.3390/ijerph16071196

Cited by 32 publications

(25 citation statements)

References 34 publications

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“…[37, 38] The ratio spectra and their derived spectra permit the quantification of one component that is present alongside another component, as well as with excipients without their interference. [37–41]…”

Section: Resultsmentioning

confidence: 99%

“…This provides an opportunity to quantify one analyte in the presence of another analyte, as well as excipients, which generally cause interference during analysis. [38–41] In the second method, the ratio spectra of different concentrations were converted into first-derivative spectra using 4 nm as a derivatization wavelength (Δλ). Different wavelengths (2 nm, 4 nm, 8 nm, and 10 nm) were tested; however, 4 nm showed the most accurate results.…”

Section: Resultsmentioning

confidence: 99%

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Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

et al. 2019

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Recently, the United States Food and Drug Administration approved a new oral dosage preparation of amlodipine besylate (AML) and celecoxib (CEL) for the management of hypertension and osteoarthritis. However, no simultaneous estimation procedures for these two analytes have been described. Hence, two simple, accurate, and precise ultraviolet spectroscopic procedures that manipulated the ratio spectra were established for concurrent quantification of AML and CEL using ethanol as a solvent. The first method involves determining the peak-to-trough amplitude difference of the ratio spectra of AML and CEL. The second method involves determining the peak amplitude of the ratio first derivative (Δλ 4 nm) spectra of AML and CEL at 334.2 nm and 254.2 nm, correspondingly. Both methods showed linearity in the range of 1–6 μg mL-1 for AML and 5–40 μg mL-1 for CEL with an excellent correlation coefficient (<0.999). The proposed procedures were validated by following the International Conference on Harmonization guidelines for accuracy, precision, selectivity, recovery, and stability studies. It is evident from the low %RSD and %RE that both analytical procedures were found to be accurate and precise, respectively. The percent recovery of AML and CEL from the formulation was found to be 99.79% and 99.34% using the ratio-difference method and 100.13% and 99.70% using the ratio first-derivative method, with a low percent relative standard deviation. Further, the proposed techniques permit concurrent quantification of AML and CEL in different concentration ratios without interference from each other; hence, these techniques can be adopted for regular quality-control studies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

et al. 2019

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“…is increases the resolution of multicomponent spectra and enhances specificity and sensitivity. Furthermore, this feature allows for the determination of the concentration of one component in the presence of others by measuring the amplitude of derivative spectra at zerocrossing wavelengths, where one of the components will have zero absorbance [36,37]. e fixed-dose formulations of AMD and COX are available in three different ratios: 2.5 : 200, 5 : 200, and 10 : 200 mg/tablet, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…However, derivatization of zeroorder spectra will allow us to analyze these components in the presence of each other, by measuring the peak amplitude of derivative spectra at zero crossings for one of the analyte. Furthermore, derivatization of ratio spectra can also be used to avoid the interference by another component and tablet excipients [36][37][38][39][40]. Hence, an attempt has been made in this proposal to develop two economical, simple, accurate, and precise derivative UV spectrophotometric methods for simultaneous determination of AMD and COX in pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Development of UV Spectrophotometric Procedures for Determination of Amlodipine and Celecoxib in Formulation: Use of Scaling Factor to Improve the Sensitivity

Attimarad

Venugopala

Al‐Dhubiab

et al. 2019

Journal of Spectroscopy

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FDA has recently approved a new fixed-dose combination of amlodipine besylate (AMD) and celecoxib (COX) for the treatment of hypertension and osteoarthritis. No analytical method has been reported for analysis of these two analytes so far. Hence, to monitor the quality and quantity in the formulation of AMD and COX a simple, accurate, precise, economical, and eco-friendly spectroscopic analytical method has been established. The first method involves the determination of AMD and COX by the first derivative UV spectroscopic method with scaling factor 10. However, the second method was based on the direct measurement of UV absorbance of AMD at 364.3 nm and ratio first derivative UV spectroscopic method for COX. Both methods showed good linearity in the range of 5 to 40 μg/ml for COX, whereas AMD showed linearity in the range of 0.5 to 10 μg/ml in first derivative method with scaling factor 10 and 1 to 10 μg/ml in the second method with good correlation coefficient (R2 > 0.998). Both the methods were validated for LOD, LOQ, accuracy, precision, recovery studies, and stability as per the ICH guidelines, and the validated results were well within the acceptable range. Both the methods were successfully utilized for the determination of AMD and COX in the presence of each other in the formulation, and statistically compared between the proposed methods. Therefore, the proposed procedures can be utilized for regular quality control studies.

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“…[5][6][7] Hence, diclofenac sodium and moxifloxacin ophthalmic solutions are used simultaneously after the cataract surgery. Several analytical methods such as spectrophotometry, [8][9][10][11][12] RP-HPLC [13][14][15][16][17][18][19][20][21][22][23] and electroanalytical methods [24][25][26] were reported in the literature for the quantification of DCL and MOX alone or with other drugs. However, the simultaneous determination of DCL and MOX has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Eco-friendly Derivative UV Spectrophotometric Methods for Simultaneous Determination of Diclofenac Sodium and Moxifloxacin in Laboratory Mixed Ophthalmic Preparation

Chohan¹,

Elgorashe²,

Balgoname³

et al. 2019

IJPER

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Background: Diclofenac Sodium (DCL) and Moxifloxacin HCl (MOX) were simultaneously used after cataract surgery to reduce the post-operative inflammation and to control infection respectively. Objectives: Three simple, accurate, eco-friendly and reproducible UV spectroscopic methods were established for concurrent determination of diclofenac sodium and moxifloxacin in ophthalmic preparation without prior separation. Methods: The first technique was established on the measurement of a peak amplitude of the first derivative spectra at the zero-crossing wavelength of one analyte. The second method was the determination of peak amplitude difference between peak and trough of ratio spectra. The third method involves the measurement of the peak amplitude of the first derivative of ratio spectra. Water has been used as a solvent. Results: The analytes exhibited good linearity in the range of 1-15 µg/mL for DCL and 1-18 µg/mL for MOX with excellent correlation coefficient (r 2 >0.999). Low percent relative standard deviation confirmed the precision of the methods. Excellent recovery with low percent relative error proved the accuracy of the methods. The specificity of the methods was evaluated by analyzing the laboratory prepared solutions of DCL and MOX. Conclusion: Proposed three techniques were effectively utilized for the simultaneous determination of DCL and MOX from ophthalmic preparation. The outcomes of the proposed procedures were compared with the earlier described methods and no statistical difference was found between the methods in terms of accuracy and precision.

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Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations

Cited by 32 publications

References 34 publications

Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

Development of UV Spectrophotometric Procedures for Determination of Amlodipine and Celecoxib in Formulation: Use of Scaling Factor to Improve the Sensitivity

Eco-friendly Derivative UV Spectrophotometric Methods for Simultaneous Determination of Diclofenac Sodium and Moxifloxacin in Laboratory Mixed Ophthalmic Preparation

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