Development of UV Spectrophotometric Procedures for Determination of Amlodipine and Celecoxib in Formulation: Use of Scaling Factor to Improve the Sensitivity

Attimarad, Mahesh; Venugopala, Katharigatta N.; Al‐Dhubiab, Bandar E.; Nair, Anroop B.; Sreeharsha, Nagaraja; Shinu, Pottathil; Akrawi, Sabah H.

doi:10.1155/2019/8202160

Cited by 15 publications

(11 citation statements)

References 32 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the straight UV absorption technique for the simultaneous quantification of both analytes is challenging without prior separation. Such binary mixtures can be quantified by eliminating interference from one another using possible modification procedures such as derivative, ratio spectroscopic, and ratio subtraction methods [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. To increase the sensitivity of the low concentration, the BEN scaling factor was optimized.…”

Section: Resultsmentioning

confidence: 99%

“…Derivative spectroscopic methods allow quantification of compounds having complete overlapping spectra by computing the peak height at the zero-crossing point. The ratio derivative spectroscopic method removes the effect of one of the components by dividing the combined spectra of analytes with the spectrum of another component [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. Hence, derivative and ratio derivative spectrophotometric methods were developed in the present work.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sensitivity Enhanced Ecofriendly UV Spectrophotometric Methods for Quality Control of Telmisartan and Benidipine Formulations: Comparison of Whiteness and Greenness with HPLC Methods

Chohan

Attimarad

Venugopala

et al. 2022

IJERPH

Self Cite

View full text Add to dashboard Cite

The development of an environmentally friendly analytical technique for simultaneous measurement of medicines with large concentration differences is difficult yet critical for environmental protection. Hence, in this work, new manipulated UV-spectroscopic methods with high scaling factors were established for concurrent quantification of telmisartan (TEL) and benidipine (BEN) in fixed-dose combinations. Two different methods were developed and established by calculation of peak height at zero crossing point of second derivative and the ratio of first derivative spectra with a scaling factor of 200 and 100, respectively. The absorption difference between the peaks and troughs of the ratio spectra, as well as continuous subtraction from ratio spectra, were established as additional methods. In addition, new procedures were validated using ICH recommendations. The proposed methods’ linearity curves were constructed in the range of 0.5–10 µg mL−1 and 1–30 µg mL−1 for BEN and TEL, respectively, under optimized conditions. Furthermore, both the detection (0.088–0.139 µg mL−1 for BEN and 0.256–0.288 µg mL−1 for TEL) and quantification limits (0.293–0.465 µg mL−1 for BEN and 0.801–0.962 µg mL−1 for TEL) were adequate for quantifying both analytes in the formulation ratios. The accuracy and precision were confirmed by the good recovery percent (98.37%–100.6%), with low percent relative error (0.67%–1.70%) and less than 2 percent relative standard deviation, respectively. The specificity of the methods was proven by accurate and precise outcomes from the standard addition method and analysis of laboratory mixed solutions with large differences in concentrations of both analytes. Finally, the BEN and TEL content of the formulations was determined simultaneously without prior separation using these first ever reported spectroscopic methods. Furthermore, developed UV derivative spectroscopic methods demonstrated high greenness and whiteness when compared to the reported HPLC methods. These findings show that the projected methods were effective, practical, and environmentally acceptable for quality control of BEN and TEL in multicomponent formulations.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sensitivity Enhanced Ecofriendly UV Spectrophotometric Methods for Quality Control of Telmisartan and Benidipine Formulations: Comparison of Whiteness and Greenness with HPLC Methods

Chohan

Attimarad

Venugopala

et al. 2022

IJERPH

Self Cite

View full text Add to dashboard Cite

show abstract

“…Scientists have reported different analytical methods for the quantification of the above fixed dose combination in synthetic mixtures, pharmaceutical formulation and biological fluids. For instance, UV [8][9][10][11][12] , TLC 13 , HPLC [14][15][16] and LC-MS/MS 17 methods have been developed. Overall, 10 studies have been reported for the estimation of Celecoxib and Amlodipine simultaneously among which only two papers had described quantification of the two drugs in biological samples using HPLC and LC-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Novel Validated LC-MS/MS Method For Simultaneous Estimation of Celecoxib and Amlodipine in Rat Plasma and Its Application to A Pharmacokinetic Study

Eswarudu¹

2022

Journal of Pharmaceutical Negative Results

View full text Add to dashboard Cite

The combination of celecoxib (CLX) and Amlodipine (AMD) was approved for hypertensive patients with osteoarthritis by US-FDA. Hence, a potential analytical method that can simultaneously quantify these two drugs is required. In view of this, a novel and fully validated liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method has been established for the quantification of CLX and AMD in rat plasma simultaneously. protein precipitation extraction technique was employed for the extraction of analytes and their deuterated analogues from rat plasma quantitatively. The analytes were separated using the mobile phase comprising of acetonitrile–water with 0.1% formic acid buffer (70:30 v/v) and a flow-rate of 1.0 mL/min and 10 min run time on Agilent SB-C18 analytical column. The multiple reaction monitoring transitions, m/z 504.7→98.1 for CLX, 492.8→129.3 for AMD; 385.6→102.8 for CLX-D4 and 496.8.5→412.3 for AMD-D4 were utilized for the analysis in order to attain high selectivity. The method showed good sensitivity and linearity in the range of the concentration 20–800 ng/mL for CLX and 0.25–10 ng/mL for AMD respectively. Moreover, the method also displayed decent accuracy (87.9-100.27% and 99.28-103.26%) for CLX and AMD and precision according to US-FDA guidelines. The precision values for inter- and intra-day were between 1.92.02-7.085% and 0.083-3.43% and for CLX and AMD respectively. Further, the results of the pharmacokinetic parameters including Cmax, tmax, AUC0-t, AUC0-∞ and t1/2 values of drugs indicated that the developed method is valuable for the successful quantification of the analytes in rat plasma. The developed method is significant and is useful for simultaneous quantification of CLX and AMD.

show abstract

“…Telmisartan, olmesartan, and irbesartan are available on the market in combination with amlodipine and hydrochlorothiazide (Figure 1), manufactured by the same or different pharmaceutical industries [4]. Different analytical methods such as spectrophotometric [11][12][13][14][15][16][17][18], derivative spectrofluorimetric [19], HPLC [20][21][22][23][24][25][26][27], electrophoresis [28], and HPTLC [29] were reported for the concurrent determination of sartans, amlodipine, or hydrochlorothiazide from formulations and biological fluids. The literature has also reported a few analytical methods for the concurrent determination of ternary mixtures of sartans, amlodipine, and hydrochlorothiazide [30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

A Rapid HPLC Method for the Concurrent Determination of Several Antihypertensive Drugs from Binary and Ternary Formulations

et al. 2021

Self Cite

View full text Add to dashboard Cite

A rapid, synchronized liquid chromatographic method was established for the estimation of hydrochlorothiazide (HCZ), amlodipine (AMD), olmesartan (OLM), telmisartan (TEL), and irbesartan (IRB) in binary and ternary coformulations using the same chromatographic conditions. Five analytes were separated on a Zorbax C18 column using isocratic elution with a mobile phase consisting of acetonitrile, methanol, and 20 mM phosphate buffer (pH 3.5) in a ratio of 45:20:35% v/v. The analytes were detected at a wavelength of 230 nm at ambient temperature. Furthermore, the proposed liquid chromatographic procedure was validated for linearity, precision, accuracy, stability, and robustness using an experimental design. Analytes were separated with good resolution within 3.5 min. Analytes showed good linearity in a concentration satisfactory to analyze the different ratios of these analytes in the formulations. Pareto charts showed that the flow rate and mobile phase composition have a significant effect on the peak area of analytes and hence need to be carefully controlled, however, the method is robust. Finally, the different formulations consisting of HCZ, AMD, OLM, TEL, and IRB in different ratios were analyzed with high accuracy using an optimized HPLC method and compared with reported methods. Furthermore, the reported HPLC procedure is simple, rapid, and accurate and therefore can used for regular quality control of binary and ternary formulations using the same stationary and mobile phase.

show abstract

Development of UV Spectrophotometric Procedures for Determination of Amlodipine and Celecoxib in Formulation: Use of Scaling Factor to Improve the Sensitivity

Cited by 15 publications

References 32 publications

Sensitivity Enhanced Ecofriendly UV Spectrophotometric Methods for Quality Control of Telmisartan and Benidipine Formulations: Comparison of Whiteness and Greenness with HPLC Methods

Sensitivity Enhanced Ecofriendly UV Spectrophotometric Methods for Quality Control of Telmisartan and Benidipine Formulations: Comparison of Whiteness and Greenness with HPLC Methods

Novel Validated LC-MS/MS Method For Simultaneous Estimation of Celecoxib and Amlodipine in Rat Plasma and Its Application to A Pharmacokinetic Study

A Rapid HPLC Method for the Concurrent Determination of Several Antihypertensive Drugs from Binary and Ternary Formulations

Contact Info

Product

Resources

About