2017
DOI: 10.1080/09540105.2017.1309361
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Development of a monoclonal antibody assay and immunochromatographic test strip for the detection of amikacin residues in milk and eggs

Abstract: An amikacin-sensitive monoclonal antibody (MAb) assay and immunochromatographic test strip were developed and applied for the detection of amikacin residues in bovine milk and chicken eggs. The immunoassay was specific to amikacin and showed no cross-reactivity with other aminoglycosides. The half maximum inhibitory concentration (IC 50 ) of the assay was 0.65 ng/mL and the results were obtained within 90 min. Recoveries from spiked food matrices were within the range of 73.55-84.61% for bovine milk and 73.70-… Show more

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Cited by 10 publications
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“…Now, simultaneous detection of several analytes using immuno­assay is attracting increasing attention. Kuang’s research group worked on the development of multi-analyte immuno­assays, i.e., the simultaneous detection of 26 sulfon­amide, 32 (fluoro)­quinolones, and so on. …”
Section: Introductionmentioning
confidence: 99%
“…Now, simultaneous detection of several analytes using immuno­assay is attracting increasing attention. Kuang’s research group worked on the development of multi-analyte immuno­assays, i.e., the simultaneous detection of 26 sulfon­amide, 32 (fluoro)­quinolones, and so on. …”
Section: Introductionmentioning
confidence: 99%