2013
DOI: 10.1089/mab.2012.0099
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Development of a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for Tetrabromobisphenol A

Abstract: Tetrabromobisphenol A (TBBPA) has been an important brominated flame retardant worldwide and has become a widely concerned environmental pollutant due to its persistence in the environment. In this study, a monoclonal antibody (MAb, designated 3D9G6) against TBBPA was produced, and an indirect competitive enzyme-linked immunoassay (icELISA) for detecting trace TBBPA was developed. The limit of detection and the half maximum inhibition concentration of TBBPA in phosphate-buffered saline were 0.8 and 3.87 ng·mL(… Show more

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Cited by 7 publications
(9 citation statements)
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“…Compared to the heterologous competitive ELISA with alpaca antiserum (IC 50 = 500 ng mL –1 ), the sensitivity was increased around 1000-fold in the VHH ELISA. In contrast with ELISAs based on PAbs 16 and mAbs, 18 the sensitivities were increased approximately 2-fold and 9-fold, respectively. Instead of using the solubilized VHH in the ELISA, displaying the VHH on phage particles showed a similar sensitivity (IC 50 = 0.46 ng mL –1 ) to VHH ELISA, but a narrower linear range (0.19–1.07 ng mL –1 ) than the latter.…”
Section: Resultsmentioning
confidence: 76%
See 1 more Smart Citation
“…Compared to the heterologous competitive ELISA with alpaca antiserum (IC 50 = 500 ng mL –1 ), the sensitivity was increased around 1000-fold in the VHH ELISA. In contrast with ELISAs based on PAbs 16 and mAbs, 18 the sensitivities were increased approximately 2-fold and 9-fold, respectively. Instead of using the solubilized VHH in the ELISA, displaying the VHH on phage particles showed a similar sensitivity (IC 50 = 0.46 ng mL –1 ) to VHH ELISA, but a narrower linear range (0.19–1.07 ng mL –1 ) than the latter.…”
Section: Resultsmentioning
confidence: 76%
“… 16 , 17 In an earlier work, the haptens of TBBPA were synthesized, and the PAb-based immunoassay was developed, with a half-maximum signal inhibition concentration (IC 50 ) of 0.87 ng mL –1 . 16 The biotin–streptavidin-amplified PAb-based and the mAb-based immunoassays for TBBPA showed an IC 50 of 0.58 ng mL –1 17 and 3.87 ng mL –1 , 18 respectively.…”
Section: Introductionmentioning
confidence: 99%
“…In some cases, such as Nb against 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47, molecular weight 485.79 Da) [ 62 ], the sensitivity of Nb-based icELISA (IC 50 = 1.4 ng/mL) is even higher than that of mAb (IC 50 = 1.75 ng/mL) [ 63 ]. In another study, Nb against tetrabromobisphenol-A (TBBPA, molecular weight 543.9 Da) [ 57 ], also exhibits higher sensitivity (IC 50 = 0.4 ng/mL) by means of the icELISA method, compared with that of mAb (IC 50 = 3.87 ng/mL) [ 64 ] and pAb (IC 50 = 0.87 ng/mL) [ 65 ].…”
Section: Performance Of Nbs In the Field Of Chemical Analysismentioning
confidence: 99%
“…Immunoassays have been developed to detect TBBPA in soil, as well as human samples. The selection of VHH antibodies [35] was conducted using knowledge gained from prior work in isolating highly sensitive pAbs [36] and mAbs [37] to the same target. In addition, this work utilized the repertoire of previously synthesized haptens to systematically pan the phage-displayed VHH library against the homologous hapten and two heterologous haptens.…”
Section: Summary Of Vhh Assays To Environmental Chemicalsmentioning
confidence: 99%