Development of a monocyte/macrophage-like cell line, RTS11, from rainbow trout spleen

Ganassin, Rosemarie C.; Bols, Niels C.

doi:10.1006/fsim.1998.0153

Cited by 187 publications

(114 citation statements)

References 25 publications

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“…The expression of MCSF1 was detectable in all of the cell lines examined, including the mononuclear cell line RTS-11 (29), the two fibroid cell lines RTG-2 (30) and RTL (31), and the epithelial cell line CL-6 (Fig. 8A).…”

Section: The Trout Mcsf1 Gene Is Down-regulated By Pmamentioning

confidence: 95%

“…Four rainbow trout cell lines were used: the mononuclear/macrophage cell line RTS-11 (29), the gonad fibroid cell line RTG-2 (30), the liver fibroid cell line RTL (31), and the liver epithelial cell line CL-6 (generous gift from Dr. A. Benmansour, Institut National de la Recherche Agronomique, Jouy-en-Josas, France). All trout cells were grown at 20°C in L-15 medium supplemented with 30% FCS for RTS-11 cells and 10% FCS for all other cell lines.…”

Section: Cell Lines and Cell Culturementioning

confidence: 99%

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Two Macrophage Colony-Stimulating Factor Genes Exist in Fish That Differ in Gene Organization and Are Differentially Expressed

Wang

Hanington

Belosevic

2008

The Journal of Immunology

102

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Mammalian macrophage CSF (MCSF; CSF-1) is the primary regulator of the mononuclear phagocyte lineage. We, for the first time, report the complete sequencing of five MCSF cDNAs from three fish species, rainbow trout, zebrafish, and goldfish. Despite the difference in the lengths of the MCSF transcripts, all of the fish MCSF molecules encode a signal peptide, a CSF-1 domain, a transmembrane domain, and an intracellular region. Each fish MCSF gene has a unique exon/intron structure. The primordial MCSF gene may have had a nine exon/eight intron structure. In this model, insertion of an intron in exon 6 in primitive fish created the fish type I MCSF, while the loss of this exon or part of the original exon 6 created the fish type II MCSF. Investigation of alternative splicing variants in trout suggests that no mammalian equivalent splice variants exist. The two trout MCSF genes are differentially expressed in vivo and contributed differently to the high-level expression of MCSF in spleen and head kidney. In contrast to the up-regulation of MCSF by PMA in mammals, in trout MCSF1 expression is down-regulated by PMA treatment. As in mammals, recombinant trout MCSF1 can promote the growth of head kidney leukocytes, and it up-regulates the expression of CXCR3 in head kidney macrophages, with the latter suggesting a role of MCSF in the trafficking of macrophages to sites of inflammation or injury where the CXCR3 ligands are expressed. Thus MCSF has an important role in the immune system of fish as in mammals.

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Section: The Trout Mcsf1 Gene Is Down-regulated By Pmamentioning

confidence: 95%

Section: Cell Lines and Cell Culturementioning

confidence: 99%

Two Macrophage Colony-Stimulating Factor Genes Exist in Fish That Differ in Gene Organization and Are Differentially Expressed

Wang

Hanington

Belosevic

2008

The Journal of Immunology

102

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“…NOD2a CARD domain NOD2aCARDF GCGAAGCTTTCCTACATCAGTATGAGTG NOD2abCARDR GTTCCGCGGTCATTGGTTAGATGGTGAGGG NOD2b CARD domain NOD2bCARDF GCCAAGCTTTCCTGGGGCATGCTGTTC NOD2abCARDR GTTCCGCGGTCATTGGTTAGATGGTGAGGG NACHT domain NACHTF GCCAAGCTTAGTATGGACACTGTGCTAGTCTCT NACHTR GTTCCGCGGTCAGCGATGATGCTTCCTCAC LRR domain LRRF GCGAAGCTTGCAATGGCCTACGTGCTCC LRRR GTTCCGCGGTCAGAATGTAAGCCTGGGT Real-time PCR trNOD2aF GCTGTAGCAAACTGGCTCAAA trNOD2abR CAGGTCTCATCTCCCTTGGTG trNOD2bF CTCCCGCTGAAACCTGGTCC trNOD2abR CAGGTCTCATCTCCCTTGGTG trNOD2F TCTCTCTCTAAGGCTGGGAAAC trNOD2R TTGCCAACACCATTGTCTACCA IRF3F ACTGGTCATGGTCGAGGTGGT IRF3R CACAAGTCCATCATCTCCTGCAG IRF7F GACCGTAAAATATTCAGGGCATGG IRF7R CTTCTCTGTGCGGGCTCTCATA IFNg2F CAAACTGAAAGTCCACTATAAGATCTCCA IFNg2R GGTCCAGCCTCTCCCTCAC IL-6F CCTTGCGGAACCAACAGTTTG IL-6R CCTCAGCAACCTTCATCTGGTC TNFaF CTGTGTGGCGTTCTCTTAATAGCAGCTT TNFaR CATTCCGTCCTGCATCGTTGC CATH2F ACATGGAGGCAGAAGTTCAGAAGA CATH2R GAGCCAAACCCAGGACGAGA caspase1F AAACCCATTCAACTAGTGCTG caspase1R CTTCACTGACTCCATTTCCTCC caspase2F CTGGGTGTTTGAGGCATTTGA caspase2R TCTCCTTACAGCGATGGTGGG caspase3F GCTGCTCCGCTTCGTTCGTG caspase3R CCAGGAGCCAGTCTGAGTGTT caspase6F CCAATGCTGACCGCTCCAATCT caspase6R CCCACGGCACGCCTGTAGTATGA caspase7F CTCTTCTTCATCCAGGCTTGTC caspase7R TCTTCTCGCTGAAACGTGGGT caspase8F GGAGGGCGGAGTCACATACA caspase8R GCGTCAGCACCGACAGAAT caspase9F CTTTGCCTACGCCTACCACT caspase9R GCTCCTGCCTTATTTGCTT Kit (Invitrogen). The cDNA template used in the PCR was generated from RTS-11 cells [18] stimulated with 50 ng/mL IFN1 recombinant protein [19] for 4 h. The annealing temperature of the first round and nested PCR was 66 C and 68 C, respectively. Specific primer...…”

Section: Cloning and Sequencing Of Trout Nod2mentioning

confidence: 99%

Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

Chang

Wang

Nie

et al. 2011

Fish & Shellfish Immunology

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“…The mononuclear cell line RTS-11 (48) cultured overnight before any treatments or RNA preparation. The cell culture supernatant was replaced with the same medium (0.5% FCS) with or without Escherichia coli LPS (Sigma-Aldrich;, 25 g/ml), poly(I:C) (Sigma-Aldrich; 50 g/ml), or rIL-1␤ (30 ng/ml).…”

Section: Expression and Modulation Of Trout Il-1 Family Members In Thmentioning

confidence: 99%

Identification of a Novel IL-1 Cytokine Family Member in Teleost Fish

Wang

Bird

Koussounadis

et al. 2009

The Journal of Immunology

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A novel IL-1 family member (nIL-1F) has been discovered in fish, adding a further member to this cytokine family. The unique gene organization of nIL-1F, together with its location in the genome and low homology to known family members, suggests that this molecule is not homologous to known IL-1F. Nevertheless, it contains a predicted C-terminal β-trefoil structure, an IL-1F signature region within the final exon, a potential IL-1 converting enzyme cut site, and its expression level is clearly increased following infection, or stimulation of macrophages with LPS or IL-1β. A thrombin cut site is also present and may have functional relevance. The C-terminal recombinant protein antagonized the effects of rainbow trout rIL-1β on inflammatory gene expression in a trout macrophage cell line, suggesting it is an IL-1β antagonist. Modeling studies confirmed that nIL-1F has the potential to bind to the trout IL-1RI receptor protein, and may be a novel IL-1 receptor antagonist.

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Development of a monocyte/macrophage-like cell line, RTS11, from rainbow trout spleen

Cited by 187 publications

References 25 publications

Two Macrophage Colony-Stimulating Factor Genes Exist in Fish That Differ in Gene Organization and Are Differentially Expressed

Two Macrophage Colony-Stimulating Factor Genes Exist in Fish That Differ in Gene Organization and Are Differentially Expressed

Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

Identification of a Novel IL-1 Cytokine Family Member in Teleost Fish

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