Abstract:: There is an increasing number of patients with severe liver disease that requires whole organ transplantation or living-related split liver transplantation. This has resulted in a shortage of donor organs, which is particularly problematic and still awaits resolution. Bioarti cial liver BAL support systems have been developed with the aim of supporting patients with life-threatening liver disease until their liver recovers. Here, we describe a high performance three-dimensional rat hepa tocyte culture system… Show more
“…The isolated hepatocytes are similar to what was reported by Veena Sharma et al about the shape of healthy hepatocytes in the liver, 66 as well as what Hu Wei-Shou et al reported about the spheroids of rat hepatocytes in the bioartificial liver 67 and Takeshi Aoki et al about cell clusters of rat hepatocytes on polyvinyl alcohol scaffold. 68 Figure 11.Scanning electron microscope images of hepatocytes isolated from rat liver cultured in (a) and (b) Patterned alginate hydrogel and (c) and (d) Simple alginate hydrogel.…”
Section: Discussionmentioning
confidence: 99%
“…The isolated hepatocytes are similar to what was reported by Veena Sharma et al about the shape of healthy hepatocytes in the liver, 66 as well as what Hu Wei-Shou et al reported about the spheroids of rat hepatocytes in the bioartificial liver 67 and Takeshi Aoki et al about cell clusters of rat hepatocytes on polyvinyl alcohol scaffold. 68 Primary hepatocyte cells in a healthy state have a threedimensional morphology, not flat, and have a cluster state. 65 The Figure 11(a) shows that the hepatocytes are located in certain places in the patterned hydrogel, but in the simple hydrogel (Figure 11(c)), they are more evenly distributed on the entire surface.…”
Section: Sem Analysis Of Rat Hepatocytes In Ca-alginate Hydrogelsmentioning
Limitations in liver transplantation and advances in cell therapy methods motivated us to study primary hepatocytes. The main challenge in using primary hepatocytes for liver regeneration is that they lose their functionalities. We aimed to develop a controlled-shape hydrogel and apply the conditioned-media of mesenchymal stromal cells (CM-MSCs) to improve in vitro hepatocyte functions. In this experimental study, following rat hepatocyte isolation by collagenase perfusion and collection of human umbilical cord CM-MSCs, a simple and precise system called electrodeposition was used to produce the patterned alginate hydrogel. To reduce the cytopathic effects, we used an indirect electrodeposition method. For characterizing this structure, mechanical properties, Fourier-transform infrared spectroscopy (FTIR), water uptake, in-vitro degradation, and hydrogel stability were studied. Urea synthesis as a basic function of hepatocytes was assessed in five different groups. Scanning electron microscope (SEM) was utilized to evaluate the primary hepatocyte morphology and their dispersion in the fabricated structure. We observed a significant increase in urea synthesis in the presence of CM-MSCs in patterned hydrogel alginate compared to 2D culture on day 3 ( p<0.05). However, there was no significant difference in simple and patterned hydrogel on day 2. We found that the electrodeposition method is appropriate for the rapid fabricating of hydrogel structures with arbitrary patterns for 3D cell culture.
“…The isolated hepatocytes are similar to what was reported by Veena Sharma et al about the shape of healthy hepatocytes in the liver, 66 as well as what Hu Wei-Shou et al reported about the spheroids of rat hepatocytes in the bioartificial liver 67 and Takeshi Aoki et al about cell clusters of rat hepatocytes on polyvinyl alcohol scaffold. 68 Figure 11.Scanning electron microscope images of hepatocytes isolated from rat liver cultured in (a) and (b) Patterned alginate hydrogel and (c) and (d) Simple alginate hydrogel.…”
Section: Discussionmentioning
confidence: 99%
“…The isolated hepatocytes are similar to what was reported by Veena Sharma et al about the shape of healthy hepatocytes in the liver, 66 as well as what Hu Wei-Shou et al reported about the spheroids of rat hepatocytes in the bioartificial liver 67 and Takeshi Aoki et al about cell clusters of rat hepatocytes on polyvinyl alcohol scaffold. 68 Primary hepatocyte cells in a healthy state have a threedimensional morphology, not flat, and have a cluster state. 65 The Figure 11(a) shows that the hepatocytes are located in certain places in the patterned hydrogel, but in the simple hydrogel (Figure 11(c)), they are more evenly distributed on the entire surface.…”
Section: Sem Analysis Of Rat Hepatocytes In Ca-alginate Hydrogelsmentioning
Limitations in liver transplantation and advances in cell therapy methods motivated us to study primary hepatocytes. The main challenge in using primary hepatocytes for liver regeneration is that they lose their functionalities. We aimed to develop a controlled-shape hydrogel and apply the conditioned-media of mesenchymal stromal cells (CM-MSCs) to improve in vitro hepatocyte functions. In this experimental study, following rat hepatocyte isolation by collagenase perfusion and collection of human umbilical cord CM-MSCs, a simple and precise system called electrodeposition was used to produce the patterned alginate hydrogel. To reduce the cytopathic effects, we used an indirect electrodeposition method. For characterizing this structure, mechanical properties, Fourier-transform infrared spectroscopy (FTIR), water uptake, in-vitro degradation, and hydrogel stability were studied. Urea synthesis as a basic function of hepatocytes was assessed in five different groups. Scanning electron microscope (SEM) was utilized to evaluate the primary hepatocyte morphology and their dispersion in the fabricated structure. We observed a significant increase in urea synthesis in the presence of CM-MSCs in patterned hydrogel alginate compared to 2D culture on day 3 ( p<0.05). However, there was no significant difference in simple and patterned hydrogel on day 2. We found that the electrodeposition method is appropriate for the rapid fabricating of hydrogel structures with arbitrary patterns for 3D cell culture.
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