The effective content of lignin in industrial lignin determines the properties of lignin products, which is of great significance to understand the performance of lignin products. In this experiment, industrial lignin was used as raw material to prepare green drilling fluid treatment agent lignin by chromatography column, and the effect of lignin as drilling fluid additive before and after separation was evaluated. In addition, the experiment include organic chemistry knowledge, oilfield chemistry and experimental teaching, which is beneficial to improving the comprehensive experimental skills of undergraduate students and facilitating the development of green environmental protection concept and guiding the cleaner production of oil field in the future.