Development of a new microfluidic platform integrating co-cultures of intestinal and liver cell lines

Bricks, Thibault; Paullier, Patrick; Legendre, Audrey; Fleury, Marie-José; Zeller, Perrine; Merlier, Franck; Anton, Pauline M.; Leclerc, Éric

doi:10.1016/j.tiv.2014.02.005

Cited by 76 publications

(75 citation statements)

References 43 publications

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“…Just like van Midwoud et al, Bricks et al [ 23 ] developed a model to mimic the interactions between the intestine and liver. However, instead of using actual slices of the organs, they used the human carcinogenic epithelial cell line Caco-2 TC7, which is the clone of Caco-2 cells, for the intestine, and hepatocarcinoma HepG2/C3A cells for the liver.…”

Section: Reviewmentioning

confidence: 99%

“…For example, van Midwoud et al [ 22 ] REVIEW was one of the fi rst to connect a liver and intestinal organ to allow for inter-organ interactions with applications towards toxicity testing. Bricks et al [ 23 ] did a similar project using liver and intestinal cells as well.…”

Section: Intestinementioning

confidence: 99%

“…HHSteC and skin biopsies [19] Created IIDMP to evaluate dynamic organ-organ interactions of the liver and intestine HepG2/C3A and Caco-2 TC7 [23] Lung Toxicity/Drug Testing Modeling disease functions through a chip undergoing cyclic mechanical strain…”

Section: Reviewmentioning

confidence: 99%

See 2 more Smart Citations

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Caplin

Granados

James

et al. 2015

Adv Healthcare Materials

179

124

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In recent years, the exploitation of phenomena surrounding microfluidics has seen an increase in popularity, as researchers have found a way to use their unique properties to create superior design alternatives. One such application is representing the properties and functions of different organs on a microscale chip for the purpose of drug testing or tissue engineering. With the introduction of "organ-on-a-chip" systems, researchers have proposed various methods on various organ-on-a-chip systems to mimic their in vivo counterparts. In this article, a systematic approach is taken to review current technologies pertaining to organ-on-a-chip systems. Design processes with attention to the particular instruments, cells, and materials used are presented.

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Section: Reviewmentioning

confidence: 99%

Section: Intestinementioning

confidence: 99%

See 1 more Smart Citation

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Caplin

Granados

James

et al. 2015

Adv Healthcare Materials

179

124

View full text Add to dashboard Cite

show abstract

“…In one such system, the Integrated Insert in a Dynamic Microfluidic Platform (IIDMP), the intestinal compartment is simulated using Caco-2 TC7 cells, while the hepatic component is represented by HepG2C3A cells (Bricks et al, 2014). Another novel multiorgan chip model (MOC, TissUse) utilizes primary small intestinal epithelial cells (SMI-100, MatTek) and HepaRG liver spheroids to reproduce intestine liver interactions (Maschmeyer et al, 2015a,b;Materne et al, 2015).…”

Section: Chip Technologymentioning

confidence: 99%

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

Gordon

Daneshian

Bouwstra

et al. 2015

ALTEX

124

103

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SummaryModels of the outer epithelia of the human body -namely the skin, the intestine and the lung -have found valid applications in both research and industrial settings as attractive alternatives to animal testing. A variety of approaches to model these barriers are currently employed in such fields, ranging from the utilization of ex vivo tissue to reconstructed in vitro models, and further to chip-based technologies, synthetic membrane systems and, of increasing current interest, in silico modeling approaches. An international group of experts in the field of epithelial barriers was convened from academia, industry and regulatory bodies to present both the current state of the art of non-animal models of the skin, intestinal and pulmonary barriers in their various fields of application, and to discuss research-based, industry-driven and regulatory-relevant future directions for both the development of new models and the refinement of existing test methods. Issues of model relevance and preference, validation and standardization, acceptance, and the need for simplicity versus complexity were focal themes of the discussions. The outcomes of workshop presentations and discussions, in relation to both current status and future directions in the utilization and development of epithelial barrier models, are presented by the attending experts in the current report.

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“…[1][2][3] Initial reports of these devices generally focused on separations and the development of a variety of functional elements for sample manipulation and handling. 4 Over the last decade, microfluidic systems have attracted considerable interests due to their capability to integrate multiple laboratory functions into a single microfluidic chip.…”

Section: Introductionmentioning

confidence: 99%

A surface acoustic wave micropump to pump fluids from a droplet into a closed microchannel using evaporation and capillary effects

2014

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A new method for converting a microdroplet on a piezoelectric substrate into continuous fluid flow in microchannels is presented. An interdigital transducer with 27.5 MHz center frequency is fabricated on a 1280 yx-LiNbO3 piezoelectric substrate for exciting surface acoustic wave. A PDMS (Polydimethylsiloxane) microchannel is mounted on the piezoelectric substrate. One end of the microchannel is connected with water absorbing paper, while the other end of the microchannel is in touch with a droplet to be converted. The surface acoustic wave is used for controlling the evaporation velocity of the fluid in the microchannel. Part of fluid in the droplet can be entered into the microchannel and transported there due to the evaporation and capillary effects. Red dye solution is used to demonstrate the conversion of the droplet and the transportation of the fluid in the microchannel. Results show that the droplet on the piezoelectric substrate can successfully be converted into continuous fluid. The flow velocity is increased with the power of the electric signal applied to the interdigital transducer. Average flow velocity is 0.0235μl/s when the power of the electric signal is 30.0dBm. The work is helpful for piezoelectric microfluidic devices for biochemical analysis.

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Development of a new microfluidic platform integrating co-cultures of intestinal and liver cell lines

Cited by 76 publications

References 43 publications

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

A surface acoustic wave micropump to pump fluids from a droplet into a closed microchannel using evaporation and capillary effects

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