Development of a new model of transvenous thrombosis in the pig superior sagittal sinus using thrombin injection and balloon occlusion

Wang, J.; Tan, Hua-Qiao; Li, M.-H.; Sun, X.-J.; Fu, Chun-Min; Zhang, Donghui; Zhou, Biao; Xu, Hanlin; Wang, W.; Xue, Binqiang

doi:10.1016/j.neurad.2009.06.002

Cited by 14 publications

(14 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seizures are a clinical feature for approximately 12–31.9% of CVST patients and is seen especially in patients with severe CVST with cortical venous thrombosis and venous cerebral infarction 27,28 . Seizures were present in our novel model, with an incidence of 5.17%, which had not been reported in previous animal models of CVST 9–18,23 . Therefore, our novel model is more suitable for simulating severe CVST.…”

Section: Discussionmentioning

confidence: 78%

“…However, these methods can rarely produce venous sinus thrombosis, cortical venous thrombosis, cerebral venous infarction, and hemorrhage simultaneously. Furthermore, thrombosis in most animal models rarely persists beyond 1 week 9–14 . Previous CVST models have induced thrombosis in only the SSS without disseminating to other venous sinuses.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A novel severe cerebral venous thrombosis rat model based on semi‐ligation combined with ferric chloride and thrombin

Xiao

Zhao

et al. 2022

CNS Neurosci Ther

View full text Add to dashboard Cite

Aims An applicable cerebral venous sinus thrombosis (CVST) model is imperative for exploring its pathophysiology. We established a novel severe CVST model using semi‐ligation, ferric chloride, and thrombin. Methods A total of 138 male Sprague–Dawley rats were randomly divided into semi‐ligation (n = 75) and non‐semi‐ligation (n = 63) groups. A sham group (n = 46) was also included. We compared short‐term and long‐term neurological and cognitive dysfunction, mortality rates, thrombus load, venous infarction volume, the blood–brain barrier permeability, brain water content, and microglia activation among the three groups. Results Thrombi involving multiple venous sinuses appeared in all semi‐ligation rats within 2 days postoperatively. Compared with the non‐semi‐ligation group, short‐term and long‐term neurological dysfunction were more severe (p < 0.05), and thrombus weight, venous infarction volumes, and microglia activation were more significant (p < 0.05) in the semi‐ligation group. Further, the cognitive function of the semi‐ligation group significantly decreased (p < 0.05) on postoperative day 21. Cumulative mortality rates between the semi‐ligation and non‐semi‐ligation groups did not differ significantly. Conclusion Semi‐ligation combined with ferric chloride and thrombin can produce a severe CVST model with multiple venous sinus involvement, which is suitable for short‐ and long‐term neurological and cognitive dysfunction assessment.

show abstract

Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

A novel severe cerebral venous thrombosis rat model based on semi‐ligation combined with ferric chloride and thrombin

Xiao

Zhao

et al. 2022

CNS Neurosci Ther

View full text Add to dashboard Cite

show abstract

“…Wang et al are the only researchers to have described a transvenous technique to occlude the sinus. 17 They described prescreening swine with cerebral angiography and performed occlusion of the sinus in specimens with favorable anatomy, found in half of their animals. As the techniques and endovascular devices have improved over the past decade, we describe a technique to reliably thrombose the sinus and perform thrombectomy without prescreening.…”

Section: Discussionmentioning

confidence: 99%

Mechanical thrombectomy and intravascular imaging for cerebral venous sinus thrombosis: a preclinical model

Pasarikovski

Keith

et al. 2021

Journal of Neurosurgery

View full text Add to dashboard Cite

OBJECTIVEAlthough the majority of patients with cerebral venous sinus thrombosis (CVST) will improve with anticoagulation therapy, a portion of patients will either present in a comatose state or continue to deteriorate clinically despite early anticoagulation. In these cases, along with treating the underlying thrombophilia, timely thrombolysis may be beneficial. Repurposed arterial thrombectomy devices may not perform as expected in the cerebral venous sinus, and there are currently no preclinical endovascular thrombectomy (EVT) models for CVST. Contrary to arterial stroke research, preclinical models utilized to test various endovascular techniques and devices are lacking. The purpose of this research was to develop a reliable preclinical animal model for the testing of endovascular strategies to treat CVST.METHODSFive consecutive male Yorkshire swine weighing 45 kg were utilized. Thrombosis of the superior sagittal sinus was induced with a bovine thrombin injection via a microcatheter under distal balloon occlusion for 15 minutes. Combined arterial injections and superselective sinus injections confirmed the extent of thrombosis. EVT was subsequently performed using a second-generation stent retriever, followed by intravascular optical coherence tomography (OCT) imaging to assess the luminal environment after thrombectomy.RESULTSThrombosis of the superior sagittal sinus, EVT, and subsequent OCT imaging were technically successful in 4 of the 5 swine. Recanalization of the sinus with a second-generation stent retriever was successful after one attempt in 3 of 4 swine (75%), and 1 swine required two attempts. OCT imaging after thrombectomy revealed regions of residual sinus luminal thrombus despite complete angiographic recanalization. Thrombosed bridging cortical veins were also observed before draining into the sinus, along with patent cortical veins.CONCLUSIONSThe authors describe a preclinical model to assess endovascular techniques and devices for the treatment of CVST. Repurposed devices from arterial stroke may not perform as expected, given the unique features of venous sinus thrombosis. Residual bridging cortical vein thrombus and residual sinus thrombus, visualized on intravascular OCT, may be present despite complete sinus recanalization on angiography, and this may be the etiology of the poor clinical outcome despite technical success. In the setting of bridging cortical vein thrombus after successful sinus thrombectomy, direct chemical thrombolysis may be warranted to dissolve the remaining clot. This model may be helpful in developing and testing a new generation of devices designed specifically for CVST treatment.

show abstract

“…In a previous study, Wang et al () worked on establishing a cerebral sinus thrombosis animal model, where a thrombin was injected into the DSS of the pig. This work revealed bilateral parasagittal infarction as well as edema, the same as seen in humans using MRI.…”

Section: Discussionmentioning

confidence: 99%

MR imaging of the yucatan pig head and neck vasculature

Habib

Utriainen

Peduzzi-Nelson

et al. 2013

Magnetic Resonance Imaging

View full text Add to dashboard Cite

show abstract

Development of a new model of transvenous thrombosis in the pig superior sagittal sinus using thrombin injection and balloon occlusion

Cited by 14 publications

References 22 publications

A novel severe cerebral venous thrombosis rat model based on semi‐ligation combined with ferric chloride and thrombin

A novel severe cerebral venous thrombosis rat model based on semi‐ligation combined with ferric chloride and thrombin

Mechanical thrombectomy and intravascular imaging for cerebral venous sinus thrombosis: a preclinical model

MR imaging of the yucatan pig head and neck vasculature

Contact Info

Product

Resources

About