Development of a new surgical sheet containing both silk fibroin and thermoplastic polyurethane for cardiovascular surgery

Shimada, Ryo; Konishi, Hayato; Ozawa, Hideki; Katsumata, Takahiro; Tanaka, Ryou; Nakazawa, Yasumoto; Nemoto, Shintaro

doi:10.1007/s00595-017-1615-6

Cited by 11 publications

(7 citation statements)

References 19 publications

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“…At six months post-implantation, the higher SF concentration patch demonstrated progressive remodeling, including significantly higher tissue infiltration, elastogenesis and endothelialization compared with lower SF concentration [21]. In addition to rat studies outstanding results also observed with canine descending aorta replacement with a SF/TPU sheet as a patch [22].…”

Section: Introductionmentioning

confidence: 99%

Compatibility Evaluation of Non-Woven Sheet Composite of Silk Fibroin and Polyurethane in the Wet State

et al. 2018

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SF/polyurethane composite non-woven sheet was fabricated to evaluate the cardiovascular tissue engineering materials in the wet state. The compatibility and microstructure analyses were carried out on the fabricated SF/polyurethane composite non-woven sheet by thermal analysis and solid-state NMR analysis in the wet state. To evaluate the modulus of elasticity, a tensile test was performed and supported with dynamic viscoelasticity and mechanical analysis. Results showed that SF/polyurethane composites form domains within the non-woven sheet and are in a finely dispersed state while maintaining their structures at a scale of several tens of nm. Moreover, an increase of the loss tangent with low elastic modulus proved that a micromolecular interaction occurs between silk fibroin (SF) and polyurethane molecules.

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Section: Introductionmentioning

confidence: 99%

Compatibility Evaluation of Non-Woven Sheet Composite of Silk Fibroin and Polyurethane in the Wet State

et al. 2018

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“…At 6 months after implantation ( Figure 10 c,d), the thickness of the PU-SF sponge almost disappeared and was further subdivided. Many biomaterials using SF-PU composite materials have been actively studied in the cardiovascular field [ 20 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ]. However, because the PU used for the composite with SF is generally not biodegradable, it was difficult to use for coating materials in combination with biodegradable SF.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Water-Dispersed Biodegradable Polyurethane-Silk Composite Sponge Using 13C Solid-State Nuclear Magnetic Resonance as Coating Material for Silk Vascular Grafts with Small Diameters

et al. 2021

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Recently, Bombyx mori silk fibroin (SF) has been shown to be a suitable material for vascular prostheses for small arteries. In this study, we developed a softer SF graft by coating water-dispersed biodegradable polyurethane (PU) based on polycaprolactone and an SF composite sponge on the knitted SF vascular graft. Three kinds of 13C solid-state nuclear magnetic resonance (NMR), namely carbon-13 (13C) cross-polarization/magic angle spinning (MAS), 13C dipolar decoupled MAS, and 13C refocused insensitive nuclei enhanced by polarization transfer (r-INEPT) NMR, were used to characterize the PU-SF coating sponge. Especially the 13C r-INEPT NMR spectrum of water-dispersed biodegradable PU showed that both main components of the non-crystalline domain of PU and amorphous domain of SF were highly mobile in the hydrated state. Then, the small-diameter SF artificial vascular grafts coated with this sponge were evaluated through implantation experiments with rats. The implanted PU-SF-coated SF grafts showed a high patency rate. It was confirmed that the inside of the SF grafts was covered with vascular endothelial cells 4 weeks after implantation. These results showed that the water-dispersed biodegradable PU-SF-coated SF graft created in this study could be a strong candidate for small-diameter artificial vascular graft.

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“…Silk fibroin is one of the major proteins forming the silkworm cocoon, which has shown excellent biocompatibility, biodegradability and mechanical properties, thus silk fibroin is regard as a promising biomaterial in cardiac tissue engineering (33,34). SF has been applied in various forms of biopolymer-based composites as cardiac patches and has demonstrated ideal efficacy (14).…”

Section: Discussionmentioning

confidence: 99%

rGO/Silk Fibroin-Modified Nanofibrous Patches Prevent Ventricular Remodeling via Yap/Taz-TGFβ1/Smads Signaling After Myocardial Infarction in Rats

Feng

Zhao

et al. 2021

Front. Cardiovasc. Med.

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Objective: After acute myocardial infarction (AMI), the loss of cardiomyocytes and dysregulation of extracellular matrix homeostasis results in impaired cardiac function and eventually heart failure. Cardiac patches have emerged as a potential therapeutic strategy for AMI. In this study, we fabricated and produced reduced graphene oxide (rGO)/silk fibroin-modified nanofibrous biomaterials as a cardiac patch to repair rat heart tissue after AMI and investigated the potential role of rGO/silk patch on reducing myocardial fibrosis and improving cardiac function in the infarcted rats.Method: rGO/silk nanofibrous biomaterial was prepared by electrospinning and vacuum filtration. A rat model of AMI was used to investigate the ability of patches with rGO/silk to repair the injured heart in vivo. Echocardiography and stress–strain analysis of the left ventricular papillary muscles was used to assess the cardiac function and mechanical property of injured hearts treated with this cardiac patch. Masson's trichrome staining and immunohistochemical staining for Col1A1 was used to observe the degree of myocardial fibrosis at 28 days after patch implantation. The potential direct mechanism of the new patch to reduce myocardial fibrosis was explored in vitro and in vivo.Results: Both echocardiography and histopathological staining demonstrated improved cardiac systolic function and ventricular remodeling after implantation of the rGO/silk patch. Additionally, cardiac fibrosis and myocardial stiffness of the infarcted area were improved with rGO/silk. On RNA-sequencing, the gene expression of matrix-regulated genes was altered in cardiofibroblasts treated with rGO. Western blot analysis revealed decreased expression of the Yap/Taz-TGFβ1/Smads signaling pathway in heart tissue of the rGO/silk patch group as compared with controls. Furthermore, the rGO directly effect on Col I and Col III expression and Yap/Taz-TGFβ1/Smads signaling was confirmed in isolated cardiofibroblasts in vitro.Conclusion: This study suggested that rGO/silk improved cardiac function and reduced cardiac fibrosis in heart tissue after AMI. The mechanism of the anti-fibrosis effect may involve a direct regulation of rGO on Yap/Taz-TGFβ1/Smads signaling in cardiofibroblasts.

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Development of a new surgical sheet containing both silk fibroin and thermoplastic polyurethane for cardiovascular surgery

Abstract: The SF/TPU sheet had excellent mechanical properties and tissue biocompatibility. These favorable features and possible biodegradability of the SF portion warrant a long-term follow-up study.

Cited by 11 publications

References 19 publications

Compatibility Evaluation of Non-Woven Sheet Composite of Silk Fibroin and Polyurethane in the Wet State

Compatibility Evaluation of Non-Woven Sheet Composite of Silk Fibroin and Polyurethane in the Wet State

Characterization of a Water-Dispersed Biodegradable Polyurethane-Silk Composite Sponge Using 13C Solid-State Nuclear Magnetic Resonance as Coating Material for Silk Vascular Grafts with Small Diameters

rGO/Silk Fibroin-Modified Nanofibrous Patches Prevent Ventricular Remodeling via Yap/Taz-TGFβ1/Smads Signaling After Myocardial Infarction in Rats

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