Development of a novel, fast, simple, nonderivative HPLC method with direct UV measurement for quantification of memantine hydrochloride in tablets

Abstract

Fast, simple, accurate, and reproducible reverse phase‐high‐performance liquid chromatography method with direct ultraviolet measurement of memantine hydrochloride in tablets was developed, without any chemical derivatization pretreatment. Three main problems appear during chromatographic analysis of memantine: detection, achieving appropriate column retention, and limited choice of mobile phase components, as a result of memantine molecular structure. Among more than 35 tested columns, the best retention and peak symmetry yielded two C8 and three C18 columns with different characteristics, at a temperature of 30°C, mobile phase composed of 1%, v/v, acetonitrile and 99%, v/v, of 0.05–0.1% phosphoric acid or 2.5–5 mmol phosphate buffer, at flow rate of 1 mL/min and injection volume of 5 µL. The retention time of memantine was between 2.6 and 4 min. Both mobile phase concepts showed perfect linearity, precision, and accuracy. This is the first successful and reproducible direct reverse phase‐high‐performance liquid chromatography–ultraviolet quantification method for memantine.

New liquid chromatography assays for simultaneous quantification of antihypertensives atenolol and valsartan in their dosage forms

“…The emerging of new pharmaceutical formulations provokes the necessity for simple, accurate, economical, green and fast analytical techniques to be applied in quality control laboratories where time and cost are critical [36]. Moreover, minimizing toxicity with retaining method efficacy may be one of the challenging aspects in developing a safer methodology.…”

Development of a spectrophotometric technique for the quantitative determination of ademol

“…The accuracy and selectivity of chromatographic methods [7] are not in doubt, but the sustained sample preparation stage, high cost of equipment, and consumables reduce their availability.…”

“…At the moment, no information has been found regarding the availability of an express, sensitive and selective method for the quantitative determination of ademol. The described HPLC method for the quantitative determination of memantadine hydrochloride (another adamantane derivative) in tablets is characterized by high sensitivity, but at the same time, it is difficult to perform and requires expensive equipment [7]. Existing spectrophotometric methods for the quantitative determination of amantadine hydrochloride (adamantane derivative) [8][9][10] have inherent availability and selectivity, but some of them are time-consuming (for example, extractive techniques) and require additional sample preparation steps, while others use unavailable reagents.…”

Development of a Novel, Fast, Simple HPLC Method for Determination of Atorvastatin and its Impurities in Tablets

“…During the method development, we focused on choosing a selective and simple mobile phase and appropriate chromatographic in order to achieve successful, effective, rapid, and reproducible separations [23]. At the beginning, we tried to replace the acetate buffer with another less UV-absorbing inorganic part of the mobile phase, that would enable better UV-spectral analysis.…”