Development of a novel fluorapatite-forming calcium phosphate cement with calcium silicate: <i>In vitro</i> and <i>in vivo</i> characteristics

Abstract

Aim of this study was to develop a novel fluorapatite-forming calcium phosphate cement (FA-CPC) with tricalcium silicate (TCS) for endodontic applications and to examine its in vitro and in vivo characteristics. The FA-CPC powder consisted of 62.8% CaHPO4, 30.8% CaCO3, and 6.4% NaF. One part of TCS was combined with 9 parts of FA-CPC powder (FA-CPC with TCS). A 1.5 M phosphate solution was used as cement liquid. Setting time (ST), diametral tensile strength (DTS), phase composition by X-ray diffraction (XRD), and cement alkalinity were analyzed. Cement biocompatibility was assessed using rat subcutaneous model. Cement ST was 10.3±0.6 min and DTS was 3.89±0.76 MPa. XRD patterns showed that highly crystalline apatitic material was the only significant phase present and pH value was approximate 11.0. FA-CPC with TCS demonstrated similar biocompatibility as that of mineral trioxide aggregate control. These results suggest that FA-CPC with TCS may be useful for endodontic applications.

Effect of a novel fluorapatite-forming calcium phosphate cement with calcium silicate on osteoblasts in comparison with mineral trioxide aggregate

“…The present results indicate that FA-forming CPC with TCS had excellent biocompatibility, similar to that of MTA. We recently reported that FA-forming CPC with TCS and MTA were highly biocompatible with rat subcutaneous connective tissue (14). The proliferation of ROS cells observed in the present study is consistent with our previous finding.…”

“…The proliferation of ROS cells observed in the present study is consistent with our previous finding. In aqueous conditions, FA-forming CPC with TCS is almost entirely converted to FA within 24 h (14). FA typically has very high crystallinity, as compared with HA, and good biocompatibility with not only osteoblasts but also dental pulp cells, mesenchymal cells, and endothelial cells (24,25).…”

“…In addition to formation of FA, it had adequate diametral tensile strength and pH and was biocompatible with rat subcutaneous connective tissue (14). The effect of FA-forming CPC with TCS on the proliferation and alkaline phosphatase (ALP) activity of osteoblasts has not been determined.…”

Calcium silicate and calcium aluminate cements for dentistry reviewed

“…Faster bone healing after periapical surgery for an apicoectomy is desired, as well as bone fusion in some instances. Calcium phosphate cements in combination with the other calcium cements may control resorption, 301,302 so that cervical resorption of teeth can be treated. Superabsorbent polymers [303][304][305] may be useful for creating bone tissue scaffolds using the calcium silicate cements.…”