Development of a Novel Fluorophore for Real-Time Biomonitoring System

Song, Hyun-Ok; Lee, Binna; Bhusal, Ram Prasad; Park, Byounghun; Yu, Kyoungsik; Chong, Chom-Kyu; Cho, Pyo Yun; Kim, Sung Yeon; Kim, Kwang Ho; Park, Hyun

doi:10.1371/journal.pone.0048459

Cited by 18 publications

(18 citation statements)

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“…The fluorescent emission spectra also exhibited wide spectral ranges, and therefore, the presence of fluorescent latex beads could be easily detected with the emission optical filter (590 nm wavelength) as employed in the LED-based portable strip reader. This result was also consistent with the previous report of coumarin-derived dendrimer characterization 28 .…”

Section: Resultssupporting

confidence: 94%

“…Coumarin-derived dendrimer was provided by Professor Hak-Sung Kim (Wonkwang University, Korea) and characterized previously 28 . For covalent conjugation of mAb with latex and coumarin-derived dendrimer, 0.8 mL of aliphatic amine latex beads (20 nm) were washed with PBS (pH 7.4) and in the presence of 0.8 mL conjugation buffer (0.1 M sodium bicarbonate, pH 8.5), 128 μL of coumarin-derived dendrimer (1 mg/mL in DMSO) was added to latex in the conjugation buffer at RT for 1 hour with shaking.…”

Section: Methodsmentioning

confidence: 99%

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Rapid and Quantitative Detection of Zoonotic Influenza A Virus Infection Utilizing Coumarin-derived dendrimer-based Fluorescent Immunochromatographic Strip Test (FICT)

et al. 2014

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Great efforts have been made to develop robust signal-generating fluorescence materials which will help in improving the rapid diagnostic test (RDT) in terms of sensitivity and quantification. In this study, we developed coumarin-derived dendrimer-based fluorescent immunochromatographic strip test (FICT) assay with enhanced sensitivity as a quantitative diagnostic tool in typical RDT environments. The accuracy of the proposed FICT was compared with that of dot blot immunoassay techniques and conventional RDTs. Through conjugation of coumarin-derived dendrimers with latex beads, fluorescent emission covering broad output spectral ranges was obtained which provided a distinct advantage of easy discrimination of the fluorescent emission of the latex beads with a simple insertion of a long-pass optical filter away from the excitation wavelength. The newly developed FICT assay was able to detect 100 ng/10 μL of influenza A nucleoprotein (NP) antigen within 5 minutes, which corresponded to 2.5-fold higher sensitivity than that of the dot blot immunoassay or conventional RDTs. Moreover, the FICT assay was confirmed to detect at least four avian influenza A subtypes (H5N3, H7N1, H7N7, and H9N2). On applying the FICT to the clinical swab samples infected with respiratory viruses, our FICT assay was confirmed to differentiate influenza H1N1 infection from other respiratory viral diseases. These data demonstrate that the proposed FICT assay is able to detect zoonotic influenza A viruses with a high sensitivity, and it enables the quantitation of the infection intensity by providing the numerical diagnostic values; thus demonstrating enhanced detectability of influenza A viruses.

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Section: Resultssupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Rapid and Quantitative Detection of Zoonotic Influenza A Virus Infection Utilizing Coumarin-derived dendrimer-based Fluorescent Immunochromatographic Strip Test (FICT)

et al. 2014

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“…As shown in Figure 6, the 14 studies incorporating qualitative methods use open-ended questionnaire items, focus groups, and/or participant interviews to investigate qualitative questions relating to treatment outcomes, 29,84,89,105,123 treatment choices, 115 patient experiences, 86,110,122 and protocol compliance. 105 One study also engages participant observation to document outcomes.…”

Section: Results Overviewmentioning

confidence: 99%

Whole Systems Research Methods in Health Care: A Scoping Review

Ijaz

Rioux²,

Elder

et al. 2019

The Journal of Alternative and Complementary Medicine

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Objectives: This scoping review evaluates two decades of methodological advances made by “whole systems research” (WSR) pioneers in the fields of traditional, complementary, and integrative medicine (TCIM). Rooted in critiques of the classical randomized controlled trial (RCT)'s suitability for evaluating holistic, complex TCIM interventions, WSR centralizes the principle of “model validity,” representing a “fit” between research design and therapeutic paradigm. Design: In consultation with field experts, 41 clinical research exemplars were selected for review from across 13 TCIM disciplines, with the aim of mapping the range and methodological characteristics of WSR studies. Using an analytic charting approach, these studies' primary and secondary features are characterized with reference to three focal areas: research method, intervention design, and outcome assessment. Results: The reviewed WSR exemplars investigate a wide range of multimodal and multicomponent TCIM interventions, typified by wellness-geared, multitarget, and multimorbid therapeutic aims. Most studies include a behavioral focus, at times in multidisciplinary or team-based contexts. Treatments are variously individualized, often with reference to “dual” (biomedical and paradigm-specific) diagnoses. Prospective and retrospective study designs substantially reflect established biomedical research methods. Pragmatic, randomized, open label comparative effectiveness designs with “usual care” comparators are most widely used, at times with factorial treatment arms. Only two studies adopt a double-blind, placebo-controlled RCT format. Some cohort-based controlled trials engage nonrandomized allocation strategies (e.g., matched controls, preference-based assignment, and minimization); other key designs include single-cohort pre–post studies, modified n-of-1 series, case series, case report, and ethnography. Mixed methods designs (i.e., qualitative research and economic evaluations) are evident in about one-third of exemplars. Primary and secondary outcomes are predominantly assessed, at multiple intervals, through patient-reported measures for symptom severity, quality of life/wellness, and/or treatment satisfaction; some studies concurrently evaluate objective outcomes. Conclusions: Aligned with trends emphasizing “fit-for-purpose” research designs to study the “real-world” effectiveness of complex, personalized clinical interventions, WSR has emerged as a maturing scholarly discipline. The field is distinguished by its patient-centered salutogenic focus and engagement with nonbiomedical diagnostic and treatment frameworks. The rigorous pursuit of model validity may be further advanced by emphasizing complex analytic models, paradigm-specific outcome assessment, inter-rater reliability, and ethnographically informed designs. Policy makers and funders seeking to support best practices in TCIM research may refer to this review ...

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“…The coumarin-derived dendrimer-based fluorescence-linked immunosorbent assay (FLISA) could detect two specific antigens of malaria: Histidine-rich protein II (HRP2) and lactate dehydrogenase (LDH). FLISA has good spectroscopic properties, such as photostability [ 146 ], and a better performance than traditional ELISA, enabling the quantification of the number of parasites in a sample even if they are present in low concentrations. Accordingly, FLISA method could be useful to detect asymptomatic cases at a modest price and with a high capacity.…”

Section: Dendrimers and Dendritic Materials In The Prevention Trementioning

confidence: 99%

Dendrimers and Dendritic Materials: From Laboratory to Medical Practice in Infectious Diseases

et al. 2020

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Infectious diseases are one of the main global public health risks, predominantly caused by viruses, bacteria, fungi, and parasites. The control of infections is founded on three main pillars: prevention, treatment, and diagnosis. However, the appearance of microbial resistance has challenged traditional strategies and demands new approaches. Dendrimers are a type of polymeric nanoparticles whose nanometric size, multivalency, biocompatibility, and structural perfection offer boundless possibilities in multiple biomedical applications. This review provides the reader a general overview about the uses of dendrimers and dendritic materials in the treatment, prevention, and diagnosis of highly prevalent infectious diseases, and their advantages compared to traditional approaches. Examples of dendrimers as antimicrobial agents per se, as nanocarriers of antimicrobial drugs, as well as their uses in gene transfection, in vaccines or as contrast agents in imaging assays are presented. Despite the need to address some challenges in order to be used in the clinic, dendritic materials appear as an innovative tool with a brilliant future ahead in the clinical management of infectious diseases and many other health issues.

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Development of a Novel Fluorophore for Real-Time Biomonitoring System

Cited by 18 publications

References 64 publications

Rapid and Quantitative Detection of Zoonotic Influenza A Virus Infection Utilizing Coumarin-derived dendrimer-based Fluorescent Immunochromatographic Strip Test (FICT)

Rapid and Quantitative Detection of Zoonotic Influenza A Virus Infection Utilizing Coumarin-derived dendrimer-based Fluorescent Immunochromatographic Strip Test (FICT)

Whole Systems Research Methods in Health Care: A Scoping Review

Dendrimers and Dendritic Materials: From Laboratory to Medical Practice in Infectious Diseases

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