Development of a novel phenotyping method to assess downy mildew symptoms on grapevine inflorescences

Buonassisi, D.; Cappellin, Luca; Dolzani, C.; Velasco, Riccardo; Peressotti, E.; Vezzulli, S.

doi:10.1016/j.scienta.2018.03.023

Cited by 14 publications

(16 citation statements)

References 37 publications

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“…Interesting and trustworthy results are gained since genotypes with a known level of susceptibility or resistance were comparatively evaluated. Previously, other authors [ 21 , 29 , 31 , 51 , 52 , 53 ] likewise included Solaris, Regent, V. riparia , Chardonnay, and Cabernet Sauvignon in their studies with leaf discs, although their scales for determining the level of susceptibility slightly differ one from another. Nonetheless, all these results are similar (or the same) and comparable.…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, all these results are similar (or the same) and comparable. More precisely, Regent was characterised as resistant (class > 7) [ 31 ] and partially resistant [ 21 , 29 , 51 ], whereas Solaris was partially resistant [ 21 , 29 ]. Regarding the North American species, V .…”

Section: Discussionmentioning

confidence: 99%

“…Leaf disc bioassay is based on the International Organisation of Vine and Wine (OIV) descriptor 452-1 (Leaf: degree of resistance to Plasmopara (leaf disc test)) [ 30 ]. Leaf disc test is widely accepted and used by many authors [ 22 , 31 , 32 , 33 ] whose aim is to distinguish levels of susceptibility to downy mildew between different varieties. When this method is properly performed, it is reliable and useful for predicting each variety’s susceptibility to downy mildew in field conditions [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging

Štambuk

Šikuten

Preiner

et al. 2021

Plants

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In the era of sustainable grapevine production, there is a growing demand to define differences between Vitis vinifera varieties in susceptibility to downy mildew. Croatia, as a country with a long tradition of grapevine cultivation, preserves a large number of native grapevine varieties. A leaf disc bioassay has been conducted on 25 of them to define their response to downy mildew, according to the International Organisation of Vine and Wine (OIV) descriptor 452-1, together with the stress response of the leaf discs using chlorophyll fluorescence and multispectral imaging with 11 parameters included. Time points of measurement were as follows: before treatment (T0), one day post-inoculation (dpi) (T1), two dpi (T2), three dpi (T3), four dpi (T4), six dpi (T5), and eight dpi (T6). Visible changes in form of developed Plasmopara viticola (P. viticola) sporulation were evaluated on the seventh day upon inoculation. Results show that methods applied here distinguish varieties of different responses to downy mildew. Based on the results obtained, a phenotyping model in the absence of the pathogen is proposed, which is required to confirm by conducting more extensive research.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging

Štambuk

Šikuten

Preiner

et al. 2021

Plants

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“…The low accuracy of the OIV scale in representing the real effect of a resistant variety on slowing down DM epidemics in the vineyard is also confirmed by some contrasting results of previous works. In some works, a relationship was found between resistance levels from visual observation of leaf disc in bioassays and from field assessments (Sotolář, 2007; Boso et al, 2014; Zyprian et al, 2016; Buonassisi et al, 2018; Foria et al, 2018; Vezzulli et al, 2018). In other works, no relationship was found and this was imputed to the inoculation of a single P. viticola strain, which may be not representative of a field population (Cadle-Davidson, 2008), or to high inoculum concentration and optimal environmental conditions of the leaf discs bioassay, which may lead to an underestimation of the resistance level with the OIV scale (Calonnec et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Resistance Components for Improved Phenotyping of Grapevine Varieties Resistant to Downy Mildew

et al. 2019

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Grapevine varieties showing partial resistance to downy mildew, caused by Plasmopara viticola, are a promising alternative to fungicides for disease control. Resistant varieties are obtained through breeding programs aimed at incorporating Rpv loci controlling the quantitative resistance into genotypes characterized by valuable agronomic and wine quality traits by mean of crossing. Traditional phenotyping methods used in these breeding programs are mostly based on the assessment of the resistance level after artificial inoculation of leaf discs in bioassays, by using the visual score proposed in the 2nd Edition of the International Organization of Vine and Wine (OIV) Descriptor List for Grape Varieties and Vitis species (2009). In this work, the OIV score was compared with an alternative approach, not used for the grapevine-downy mildew pathosystem so far, based on the measurement of components of resistance (RCs); 15 grapevine resistant varieties were used in comparison with the susceptible variety ‘Merlot’. OIV scores were significantly correlated with P. viticola infection frequency (IFR), the latent period for the downy mildew (DM) lesions to appear (LP50), and the number of sporangia produced per lesion (SPOR), so that when the OIV score increased (i.e., the resistance level increases), IFR and SPOR decreased, while LP50 increased. The relationship was linear for LP50, monomolecular for IFR and hyperbolic for SPOR. No significant correlation was found between OIV score and DM lesion size, sporangia produced per unit area of lesion, length of infectious period, and infection efficiency of the sporangia produced on DM lesions. The correlation between OIV score and area under the disease progress curve (AUDPC) calculated by using the RCs and a simulation model was significant and fit an inverse exponential function. Based on the results of this study, the measurement of the RCs to P. viticola in grapevine varieties by means of monocyclic, leaf disc bioassays, as well as their incorporation into a model able to simulate their effect on the polycyclic development of DM epidemics in vineyards, represents an improved method for phenotyping resistance level.

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“…All progeny individuals were evaluated for their degree of resistance against downy and powdery mildew. Firstly, P. viticola spores were inoculated on potted seedlings following a well-established protocol [6]; secondly, prior to plant treatment and recovery, E. necator artificial infection was caused by naturally infected plants adjacent to the potted seedlings. The assessments were recorded respectively at 7 and 14 days post-inoculation (dpi) based on the OIV 452 and 455 descriptors.…”

Section: Phenotypic Screeningmentioning

confidence: 99%

Marker-assisted breeding for Downy mildew, Powderey mildew and Phylloxera resistance at FEM

et al. 2019

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Il programma di miglioramento genetico per le resistenze a stress biotici ha avuto inizio presso la Fondazione Edmund Mach (FEM) nel 2010. Inizialmente è stata condotta una caratterizzazione sia genotipica che fenotipica di materiali acquisiti da altri programmi di breeding e di materiale selvatico raccolto in New Jersey. Sia i genotipi conosciuti nei database internazionali che i genotipi sconosciuti, imparentati e non, sono stati impiegati come linee parentali nel processo di introgressione e di piramidazione di loci di interesse. Una volta pianificati e ottenuti gli incroci, la valutazione delle progenie è avvenuta seguendo un processo di Marker-Assisted Selection: dapprima è avvenuta la selezione fenotipica in serra in base al tipo di malattia e al numero di loci attesi per la medesima malattia; successivamente si è proceduto con lo screening molecolare in base ai loci specifici attesi nei parentali. Cinque sono i loci Run/Ren associati alla resistenza all'oidio presenti nel programma FEM; riguardo ai loci associati alla resistenza alla peronospora, quattro Rpv sono ben rappresentati nel piano di incroci. Ad oggi il 26% delle F1 è piramidizzato per quattro loci di resistenza.

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Development of a novel phenotyping method to assess downy mildew symptoms on grapevine inflorescences

Cited by 14 publications

References 37 publications

Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging

Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging

Assessment of Resistance Components for Improved Phenotyping of Grapevine Varieties Resistant to Downy Mildew

Marker-assisted breeding for Downy mildew, Powderey mildew and Phylloxera resistance at FEM

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