Development of a novel resin with antimicrobial properties for dental application

Abstract

The adhesion of biofilm on dental prostheses is a prerequisite for the occurrence of oral diseases.ObjectiveTo assess the antimicrobial activity and the mechanical properties of an acrylic resin embedded with nanostructured silver vanadate (β-AgVO3).Material and MethodsThe minimum inhibitory concentration (MIC) of β-AgVO3 was studied in relation to the species Staphylococcus aureus ATCC 25923, Streptococcus mutans ATCC 25175, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 10231. The halo zone of inhibition method was performed in triplicate to determine the inhibitory effect of the modified self-curing acrylic resin Dencor Lay - Clássico®. The surface hardness and compressive strength were examined. The specimens were prepared according to the percentage of β-AgVO3 (0%-control, 0.5%, 1%, 2.5%, 5%, and 10%), with a sample size of 9x2 mm for surface hardness and antimicrobial activity tests, and 8x4 mm for the compression test. The values of the microbiologic analysis were compared and evaluated using the Kruskal-Wallis test (α=0.05); the mechanical analysis used the Shapiro-Wilk's tests, Levene's test, ANOVA (one-way), and Tukey's test (α=0.05).ResultsThe addition of 10% β-AgVO3 promoted antimicrobial activity against all strains. The antimicrobial effect was observed at a minimum concentration of 1% for P. aeruginosa, 2.5% for S. aureus, 5% for C. albicans, and 10% for S. mutans. Surface hardness and compressive strength increased significantly with the addition of 0.5% β-AgVO3 (p<0.05). Higher rates of the nanomaterial did not alter the mechanical properties of the resin in comparison with the control group (p>0.05).ConclusionsThe incorporation of β-AgVO3 has the potential to promote antimicrobial activity in the acrylic resin. At reduced rates, it improves the mechanical properties, and, at higher rates, it does not promote changes in the control.

Antimicrobial properties of acrylic resins for dentures impregnated with silver nanoparticles

“…The results showed a positive effect of antimicrobial activity of the impregnated material on Staphylococcus aureus because the inhibitory effect was greater with the increasing concentration AgNPs. The results are consistent with the findings of Castro et al who have shown that the addition of 5% and 10% β-AgVO3 significantly reduces the metabolic activity of the Pseudomonas aeruginosa and Staphylococcus aureus in both types of polymerization of the acrylate denture base [13][14][15][16][17][18][19] .…”

“…Compared with other metals that are a part of nanoparticles, silver in low concentrations shows weak toxic effects on human cells [12][13][14][15] . Studies have shown that the positively charged Ag+ ion is responsible for its antimicrobial activity, which allows the binding of the negatively charged electrostatic bacterial cell membrane and the positively charged nanoparticles 16,17 .…”

“…U poređenju sa drugim metalima koji su u sastavu nanočestica, srebro u niskim koncentracijama pokazuje slab toksični efekat na ćelije čoveka [12][13][14][15] . Istraživanja su pokazala da je za antimikrobnu aktivnost zaslužan pozitivno naelektrisani Ag+ jon, koji omogućava elektrostatsko vezivanje negativno naelektrisane bakterijske ćelijske membrane i pozitivno naelektrisanih nanočestica 16,17 .…”

“…Dobijeni rezultati su u saglasnosti sa nalazima Castro i sar. koji su pokazali da dodatak 5% i 10% β-AgVO3 značajno smanjuje metaboličku aktivnost Pseudomonasa aeruginosa i Staphylococcusa aureusa u oba tipa polimerizacije akrilata za bazu zubne proteze [13][14][15][16][17][18][19] . Slane i sar.…”

Incorporating Antimicrobial Nanomaterial and its Effect on the Antimicrobial Activity, Flow and Radiopacity of Endodontic Sealers

“…The development of nanostructured silver vanadate decorated with AgNPs (AgVO3) can prevent agglomeration. Dental acrylic resins incorporating this nanomaterial have demonstrated antimicrobial activity against the microorganisms P. aeruginosa, C. albicans, S. mutans and S. aureus (12).…”