Development of a peptide aptamer pair-linked rapid fluorescent diagnostic system for Zika virus detection

Nguyen, Anh Thi Viet; Duong, Bao Tuan; Park, Hyun; Yeo, Seon-Ju

doi:10.1016/j.bios.2021.113768

Cited by 17 publications

(9 citation statements)

References 36 publications

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“…The commercial H5-specific RDT kit commonly used in Korea (Catalog Number: RG1505MH, Bionote) also failed to detect H5 HPAIVs within the 2.3.4.4b subclade ( Figure S10 ). In the present study, we developed two novel mAbs against the 2.3.4.4b subclade and conjugated these mAbs to Eu NPs, which are well-known fluorescence materials employed to improve the sensitivity of RDT kits [ 47 , 48 , 49 , 50 ]. Thus, our FICT assay obtained an LoD of 10 3.9 EID50/test ( Figure S11 ) for the detection of H5 2.3.4.4b viruses, which was ~7.9-fold higher than that reported by Nguyen et al (2017), and 32-fold higher than that of the colloidal gold-based RDT kit using the same pair of mAbs.…”

Section: Discussionmentioning

confidence: 99%

Development of a Rapid Fluorescent Diagnostic System for Early Detection of the Highly Pathogenic Avian Influenza H5 Clade 2.3.4.4 Viruses in Chicken Stool

Bao

Than

et al. 2022

IJMS

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Rapid diagnosis is essential for the control and prevention of H5 highly pathogenic avian influenza viruses (HPAIVs). However, highly sensitive and rapid diagnostic systems have shown limited performance due to specific antibody scarcity. In this study, two novel specific monoclonal antibodies (mAbs) for clade 2.3.4.4 H5Nx viruses were developed by using an immunogen from a reversed genetic influenza virus (RGV). These mAbs were combined with fluorescence europium nanoparticles and an optimized lysis buffer, which were further used for developing a fluorescent immunochromatographic rapid strip test (FICT) for early detection of H5Nx influenza viruses on chicken stool samples. The result indicates that the limit of detection (LoD) of the developed FICT was 40 HAU/mL for detection of HPAIV H5 clade 2.3.4.4b in spiked chicken stool samples, which corresponded to 4.78 × 104 RNA copies as obtained from real-time polymerase chain reaction (RT-PCR). An experimental challenge of chicken with H5N6 HPAIV is lethal for chicken three days post-infection (DPI). Interestingly, our FICT could detect H5N6 in stool samples at 2 DPI earlier, with 100% relative sensitivity in comparison with RT-PCR, and it showed 50% higher sensitivity than the traditional colloidal gold-based rapid diagnostic test using the same mAbs pair. In conclusion, our rapid diagnostic method can be utilized for the early detection of H5Nx 2.3.4.4 HPAIVs in avian fecal samples from poultry farms or for influenza surveillance in wild migratory birds.

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Section: Discussionmentioning

confidence: 99%

Development of a Rapid Fluorescent Diagnostic System for Early Detection of the Highly Pathogenic Avian Influenza H5 Clade 2.3.4.4 Viruses in Chicken Stool

Bao

Than

et al. 2022

IJMS

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“…To identify ZIKV, Nguyen et al created a new biosensor [149] . A peptide aptamers pair (PAP) were used to create an antibody-free lateral FICT.…”

Section: Zika Detectionmentioning

confidence: 99%

Recent Advances in Biosensors for Detection of COVID-19 and Other Viruses

Patel

Surve

Parmar

et al. 2023

IEEE Rev. Biomed. Eng.

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This century has introduced very deadly, dangerous, and infectious diseases to humankind such as the influenza virus, Ebola virus, Zika virus, and the most infectious SARS-CoV-2 commonly known as COVID-19 and have caused epidemics and pandemics across the globe. For some of these diseases, proper medications, and vaccinations are missing and the early detection of these viruses will be critical to saving the patients. And even the vaccines are available for COVID-19, the new variants of COVID-19 such as Delta, and Omicron are spreading at large. The available virus detection techniques take a long time, are costly, and complex and some of them generates false negative or false positive that might cost patients their lives. The biosensor technique is one of the best qualified to address this difficult challenge. In this systematic review, we have summarized recent advancements in biosensor-based detection of these pandemic viruses including COVID-19. Biosensors are emerging as efficient and economical analytical diagnostic instruments for early-stage illness detection. They are highly suitable for applications related to

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“…To overcome the disadvantages of cross-reactivity, long preparation cycles, and the high costs of antibodies, a peptide pair-based flow immunochromatographic test strip (FICT) assay targeting the ZIKV E protein were established. 72 Here, peptide aptamer B2.33 was directly coated to the detection line of the nitrocellulose (NC) membrane as the capture peptide and europium nanoparticle-linked peptide aptamer P6.1 in the conjugate pad as the FICT detector (Fig. 2B).…”

Section: Research Advances In the Diagnosis Of Infectious Diseasesmentioning

confidence: 99%

Point-of-care testing of infectious diseases: recent advances

Shang

Guo

2023

Sens. Diagn.

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Development of a peptide aptamer pair-linked rapid fluorescent diagnostic system for Zika virus detection

Cited by 17 publications

References 36 publications

Development of a Rapid Fluorescent Diagnostic System for Early Detection of the Highly Pathogenic Avian Influenza H5 Clade 2.3.4.4 Viruses in Chicken Stool

Development of a Rapid Fluorescent Diagnostic System for Early Detection of the Highly Pathogenic Avian Influenza H5 Clade 2.3.4.4 Viruses in Chicken Stool

Recent Advances in Biosensors for Detection of COVID-19 and Other Viruses

Point-of-care testing of infectious diseases: recent advances

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