Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza

Lee, Kyoung G.; Lee, Tae Jae; Jeong, Soon Woo; Choi, Ho Woon; Heo, Nam Su; Park, Jung Youn; Park, Tae Jung; Lee, Seok Jae

doi:10.3390/s120810810

Cited by 30 publications

(15 citation statements)

References 20 publications

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“…Perceptible changes in optical biosensors occur by two means: change in visual characteristics of sensing area when analyte binds with target as happens in surface plasmon resonance (SPR) or by labelling the analyte with a specific fluorescent molecule that gives visual signals. A fluorescent immunosensor was specially designed for detection of antigen or antibody in sera against A (H1N1) pdm09 [73]. In this study, the plastic based microfluidic sensor was developed that further coated with gold in detection channels of the sensor.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

et al. 2020

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H1N1 (Swine flu) is caused by influenza A virus, which is a member of Orthomyxoviridae family. Transmission of H1N1 occurs from human to human through air or sometimes from pigs to humans. The influenza virus has different RNA segments, which can reassert to make new virus strain with the possibility to create an outbreak in unimmunized people. Gene reassortment is a process through which new strains are emerging in pigs, as it has specific receptors for both human influenza and avian influenza viruses. H1N1 binds specifically with an α-2,6 glycosidic bond, which is present in human respiratory tract cells as well as in pigs. Considering the fact of fast multiplication of viruses inside the living cells, rapid detection methods need an hour. Currently, WHO recommended methods for the detection of swine flu include real-time PCR in specific testing centres that take 3–4 h. More recently, a number of methods such as Antigen–Antibody or RT-LAMP and DNA biosensors have also been developed that are rapid and more sensitive. This review describes the various challenges in the diagnosis of H1N1, and merits and demerits of conventional vis-à-vis latest methods with special emphasis on biosensors.

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Section: Optical Biosensorsmentioning

confidence: 99%

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

et al. 2020

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“…With this three-sigma rule, the detection limit was less than 1 pg/mL, significantly lower than most other studies (0.5 ng/mL to 1 µg/mL). [6][7][8] The same standard curve was constructed using a cell phone camera as an optical detector. The actual cell phone images were shown underneath the graph, for PBS and the 100-pg/mL H1N1 solution.…”

Section: Standard Curves and Lowest Detection Limitsmentioning

confidence: 99%

Rapid and Sensitive Detection of H1N1/2009 Virus from Aerosol Samples with a Microfluidic Immunosensor

et al. 2014

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Influenza A H1N1/2009 is a highly infectious, rapidly spreading airborne disease that needs to be monitored in near real time, preferably in a microfluidic format. However, such demonstration is difficult to find as H1N1 concentration in aerosol samples is extremely low, with interference from dust particles. In this work, we measured Mie scatter intensities from a microfluidic device with optical waveguide channels, where the antibody-conjugated latex beads immunoagglutinated with the target H1N1 antigens. Through careful optimizations of optical parameters, we were able to maximize the Mie scatter increase from the latex immunoagglutinations while minimizing the background scatter from the dust particles. The aerosol samples were collected from a 1:10 mock classroom using a button air sampler, where a nebulizer generated aerosols, simulating human coughing. The detection limits with real aerosol samples were 1 and 10 pg/mL, using a spectrometer or a cell phone camera as an optical detector, respectively. These are several orders of magnitudes more sensitive than the other methods. The microfluidic immunosensor readings are in concordance with the results of reverse transcription polymerase chain reaction. The assay time was 30 s for sampling and 5 min for the microfluidic assay.

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“…Fluorescence detection has advantages like: high detection sensitivity (such as single molecule detection); fast response times; localized fluorescence signal; multiplexed assays using multicolor dyes; and the straight forward labeling process, which provides appropriate functional groups [23][24][25]. Laser-induced fluorescence (LIF) applied for analyte detection is one of the most sensitive detection techniques, which is capable of reaching concentration detection below 10 −13 mol L −1 and a mass detection of less than 10 molecules.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination

Fernández‐Baldo

Ortega

Pereira

et al. 2016

Microchemical Journal

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Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza

Cited by 30 publications

References 20 publications

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

Rapid and Sensitive Detection of H1N1/2009 Virus from Aerosol Samples with a Microfluidic Immunosensor

Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination

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