Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Wang, Jianyi; Wang, Qi; Zheng, Yongjun; Peng, Tao; Yao, Kai; Xie, Su‐Yuan; Zhang, Xiya; Xia, Xi; Li, Jiancheng; Jiang, Haiyang

doi:10.1080/09540105.2017.1359498

Cited by 42 publications

(16 citation statements)

References 20 publications

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“…To date, various methods have been used to measure CAP, TAP, FF, and FFA in animal-derived foods; these methods include the fluorescent microsphere lateral flow immunoassay (FM-LFA), 7 enzyme-linked immunosorbent assay (ELISA), 8,9 reversed-phase high-performance liquid chromatography with ultraviolet detection (RP-HPLC-UVD), 10 HPLC with diode array detection (DAD), 11 RP-HPLC with fluorescence detection (FLD), 12 LC-mass spectrometry (MS), 13,14 LC tandem mass spectrometry (MS/MS), [15][16][17][18][19][20] ultra-performance liquid chromatography (UPLC)-MS/ MS, [21][22][23][24] and gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS). 25 In previous research, we developed an LC-MS/MS 20 method for the simultaneous determination of CAP, TAP, FF, and FFA in eggs.…”

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confidence: 99%

Development and comparison of liquid–liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs

et al. 2019

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Accelerated solvent extraction was investigated as a novel alternative technology for the separation and quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine from poultry eggs, and the results were compared with the results of liquid–liquid extraction. Rapid quantification of the target compounds was carried out by ultra‐performance liquid chromatography‐electrospray ionization tandem triple quadrupole mass spectrometry. This optimized method was validated according to the requirements defined by the European Union and the United States Food and Drug Administration. Finally, the new approach was successfully applied to the quantitative determination of these analytes in 90 commercial poultry eggs from local supermarkets.

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confidence: 99%

Development and comparison of liquid–liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs

et al. 2019

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“…Recently, several studies devoted to multicomponent ICAs for simultaneous detection of antibiotics, either from a single pharmacological group [29][30][31][32][33] or from different classes [30][31][32] have been proposed ( Table 1). In general, multicomponent ICAs seem to be a promising technology for effective and low-cost point-of-care screening of food products for relevant contaminants.…”

Section: Introductionmentioning

confidence: 99%

Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products

et al. 2019

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BACKGROUND Ciprofloxacin (CIP) and chloramphenicol (CAP) are relevant antibiotics of the fluoroquinolone (FQ) and amphenicol (AP) groups, respectively, widely used in veterinary practice and they contaminate agricultural products. In this study, a rapid and sensitive immunochromatographic assay (ICA) was developed for simultaneous detection of CIP and CAP in dairy products. The ICA was carried out in a direct competitive format using gold nanoparticles as a label. RESULTS The ICA developed here allowed for the detection of CIP and CAP in Triton X‐100‐containing buffered saline (PBST) within 15 min with instrumental detection limits of 20 pg mL−1 and 0.5 ng mL−1, respectively, and with a visual detection limit of 5 ng mL−1 for both antibiotics. The ICA showed cross‐reactivity (69–160%) to 19 antibiotics in the FQ group and no cross‐reactivity (<0.1%) to 2 antibiotics of the AP group. The ICA allowed detection of CIP and CAP in a panel of dairy products by employing a simple procedure of preliminary sample preparation. The detection limits for the two antibiotics were the same as in PBST. The analytical recoveries of CIP and CAP in dairy products ranged from 83% to 120%. CONCLUSION The analytical characteristics of the test system allow its use for the detection of antibiotics in milk and dairy products during all steps of production. © 2019 Society of Chemical Industry

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“…high-performance liquid chromatography coupled with UV and mass spectrometry detectors (Asea, Patterson, Korsrud, Dowling, & Boison, 2001;Jedziniak, Szprengier-Juszkiewicz, & Olejnik, 2009;Liu et al, 2015;Peng et al, 2013;van Pamel & Daeseleire, 2015). Although these methods are very accurate and sensitive, they are costly and require complex sample pretreatment steps and trained personnel (Mukunzi, Suryoprabowo, Song, Liu, & Kuang, 2018;Qu et al, 2019;Wang et al, 2018;Zeng, Jiang, Liu, Song, & Kuang, 2018). Immunoassays are superior to chromatographic methods in screening tests for their simplicity, sensitivity, and high-throughput capabilities (Chen et al, 2017; Kong et al, 2017;Wang, Luo, Chen, Huang, & Jiang, 2016).…”

Section: Introductionmentioning

confidence: 99%

Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk

Chen

Peng

Liu

et al. 2019

Food and Agricultural Immunology

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Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk, Food and Agricultural Immunology, 30:1, 320-332, ABSTRACTThe flunixin residues in foods of animal origin could pose hazards for human health. In this paper, a broad-selectivity polyclonal antibody was obtained using 5-hydroxyflunixin coupled with bovine serum albumin as the immunogen. The semi-inhibitory concentration (IC 50 ) of the polyclonal antibody was 1.43 ng/mL for flunixin and 0.29 ng/mL for 5-hydroxyflunixin, respectively, which were far below the maximum residue limits set by the United States, the European Union and China. The limits of detection were calculated to be 2.98 μg/kg for flunixin in bovine muscle and 0.78 μg/L for 5-hydroxyflunixin in milk. In spike and recovery tests, the average recovery ranged from 83% to 105%, with a satisfying coefficient of variance ranging from 5.8% to 11.3%. Furthermore, the ELISA method was validated by a well-established LC-MS/MS method. These results proved that the established method is suitable for flunixin and 5-hydroxyflunixin residue analysis. ARTICLE HISTORY

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Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Cited by 42 publications

References 20 publications

Development and comparison of liquid–liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs

Development and comparison of liquid–liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs

Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products

Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk

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