Development of a radioimmunoassay for parathion

Ercegovich, C. D.; Vallejo, Remo P.; Gettig, Russell R.; Woods, Lynn; Bogus, Edward R.; Mumma, Ralph O.

doi:10.1021/jf00105a032

Cited by 76 publications

(46 citation statements)

References 2 publications

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“…As with all small molecules, the pesticide must first be conjugated to a carrier protein to generate an immune response. A sensitive radioimmunoassay has been developed by Ercegovich et al (1981) in which parathion was coupled to protein through the nitro group using diazotization. They produced a polyclonal antiserum that was specific for parathion-ethyl and used it to measure pesticide levels in fortified plasma and plant tissues.…”

Section: Introductionmentioning

confidence: 99%

Production of a Recombinant Anti-Parathion Antibody (scFv); Stability in Methanolic Food Extracts and Comparison to an Anti-Parathion Monoclonal Antibody

Garrett

Appleford

Wyatt

et al. 1997

J. Agric. Food Chem.

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A recombinant single-chain variable fragment (scFv) antibody, specific for the organophosphate pesticide parathion-ethyl, was produced from genetic material obtained from a hybridoma cell line secreting an anti-parathion monoclonal antibody (Mab). The scFv and parent Mab showed similar, but not identical, characteristics in a microtitration plate enzyme-linked immunosorbent assay (ELISA). Limits of detection for the Mab and scFv-based ELISAs of 1.6 and 2.3 ng/well, respectively, were observed. Cross-reactions of other structurally similar organophosphates were similar in the two assays; highest cross-reactions were observed with parathion-methyl and fenitrothion (both giving 6% and 4% values for the Mab-and scFv-based assays, respectively). Performances of the assays in the presence of methanolic extracts of rice and orange peel were compared; the scFv demonstrated similar stability to the Mab at methanol concentrations up to 5% (v/v). At higher concentrations (above 10%, v/v) performance of both assays deteriorated, though the Mab-based assay was less affected than the scFv-based assay. ScFvs promise to be as useful as Mabs for the analysis of food samples by ELISA.

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Section: Introductionmentioning

confidence: 99%

Production of a Recombinant Anti-Parathion Antibody (scFv); Stability in Methanolic Food Extracts and Comparison to an Anti-Parathion Monoclonal Antibody

Garrett

Appleford

Wyatt

et al. 1997

J. Agric. Food Chem.

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“…Most work has been done on immunoassays for herbicides, but some work on the organophosphorus pesticides parathion (Ercegovich et al, 1981;Heldman et al, 1985;Ngeh-Ngwainbi et al, 1986) and malathion (Haas and Guardia, 1968;Centeno et al, 1970) and the organophosphorus nerve gas soman (Hunter et al, 1982;Buenafe and Rittenberg, 1987) has been reported. In this paper, the development of specific polyclonal and * Author to whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Mono- and polyclonal antibodies to the organophosphate fenitrothion. 1. Approaches to hapten-protein conjugation

McAdam¹,

Hill²,

Beasley³

et al. 1992

J. Agric. Food Chem.

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“…Taking the opportunity for development of an immunochemical method based on radioimmunoassay (RIA) for organophosphorus parathion by Ercegovich et al 4 , the methods, mainly ELISAs supplanted with RIAs, have been aggressively developed and applied as simple and rapid analytical means for wide-ranging pesticides in various samples as shown in numerous reviews 9,20,23,27 . However, it is unfortunate that most are restricted to in-house use, and in relative terms, the number of universally accessible commercial kit-based ELISAs remains small 7 .…”

Section: Potential Ranking Of Elisas In Pesticide Residue Analysis Anmentioning

confidence: 99%

The Present State and Perspective on Simple and Rapid Immunochemical Detection for Pesticide Residues in Crops

Watanabe

2011

JARQ

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The presence of pesticide residues in crops is a matter of global public concern, and simple, rapid, and reliable methods for pesticide residue analysis are essential to ensure food safety. In this review, I evaluate a commercially available kit-based enzyme-linked immunosorbent assay (ELISA), which uses specific or selective antigen-antibody interactions, for simple and rapid pesticide residue analysis in crops, particularly before shipment of the crops. The evaluated ELISAs were found to be sufficiently sensitive to detect three pesticides (imidacloprid, fenitrothion, and chlorothalonil) at levels close to the maximum residue limits. Simple dilution of sample extracts alone was sufficient to surmount the problem of matrix interference, which can be troublesome with ELISA. The average recovery rates of the three pesticides exceeded 84%, and the average coefficients of variation were less than 13% for all tested crop samples. The results obtained with the ELISAs correlated well with those obtained by reference chromatographic methods for all three pesticides (r > 0.96). These findings strongly suggest that ELISA is a suitable method for quantitative and reliable screening analysis of these pesticides in crops without the need for sample pretreatment. Elimination of this need can be expected to save time and money and considerably increase sample throughput.

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Development of a radioimmunoassay for parathion

Cited by 76 publications

References 2 publications

Production of a Recombinant Anti-Parathion Antibody (scFv); Stability in Methanolic Food Extracts and Comparison to an Anti-Parathion Monoclonal Antibody

Production of a Recombinant Anti-Parathion Antibody (scFv); Stability in Methanolic Food Extracts and Comparison to an Anti-Parathion Monoclonal Antibody

Mono- and polyclonal antibodies to the organophosphate fenitrothion. 1. Approaches to hapten-protein conjugation

The Present State and Perspective on Simple and Rapid Immunochemical Detection for Pesticide Residues in Crops

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