The Present State and Perspective on Simple and Rapid Immunochemical Detection for Pesticide Residues in Crops

Abstract

The presence of pesticide residues in crops is a matter of global public concern, and simple, rapid, and reliable methods for pesticide residue analysis are essential to ensure food safety. In this review, I evaluate a commercially available kit-based enzyme-linked immunosorbent assay (ELISA), which uses specific or selective antigen-antibody interactions, for simple and rapid pesticide residue analysis in crops, particularly before shipment of the crops. The evaluated ELISAs were found to be sufficiently sensitive to detect three pesticides (imidacloprid, fenitrothion, and chlorothalonil) at levels close to the maximum residue limits. Simple dilution of sample extracts alone was sufficient to surmount the problem of matrix interference, which can be troublesome with ELISA. The average recovery rates of the three pesticides exceeded 84%, and the average coefficients of variation were less than 13% for all tested crop samples. The results obtained with the ELISAs correlated well with those obtained by reference chromatographic methods for all three pesticides (r > 0.96). These findings strongly suggest that ELISA is a suitable method for quantitative and reliable screening analysis of these pesticides in crops without the need for sample pretreatment. Elimination of this need can be expected to save time and money and considerably increase sample throughput.

Quantitative analysis of fungicide azoxystrobin in agricultural samples with rapid, simple and reliable monoclonal immunoassay

“…We have considered the suitability of several kit-based ELISAs on applicability to screening method for pesticide residue in agricultural products through the above-described studies, and have come to the conclusion that every kit-based ELISAs studied are useful and practical methods for rapid and simple detection of pesticide residue, especially when judging whether residue levels overrun the maximum residue limits (MRLs) for each tested agricultural samples (Watanabe, 2011;Watanabe, Baba, & Miyake, 2011;Watanabe, Miyake, Baba, Eun, & Endo, 2006). So, it is important to study (1) evaluation of analytical characteristics of ELISA such as sensitivity and specificity, (2) influence of matrix on analytical performance and (3) securance of reliability of analytical results in order to successfully make use of handy kitbased ELISAs, and to steadily accumulate these experimental results.…”

Scientific Methods for Evaluation of Organophosphorus and Carbamate Pesticide Residues in Fruits and Vegetables: A Review

“…Furthermore, since it can stack quite a large number samples simultaneously, it empowers quick and exact evaluation of pesticide residues in agricultural things before transportation. For sure, the essential benefit of ELISA for distinguishing pesticides residue in the accommodation of sample preparation techniques (Watanabe, 2011).…”

Extraction and Identification Techniques for Quantification of Carbamate Pesticides in Fruits and Vegetables

“…Additionally, since it can load many samples concurrently, it enables rapid and precise assessment of pesticide residues in agricultural items prior to shipping. Indeed, the primary advantage of ELISA for identifying pesticide residues is the convenience of sample preparation methods [95]. Bellemjid et al [96] created a rapid ELISA to detect carbamates such as carbendazim and carbofuran using synthetic compounds with acid functions linked with BSA protein and injected into rabbits with antibodies collected for the immunoanalytical test.…”