Development of a rapid detection method for enrofloxacin in food

Enrofloxacin (ENR), as a synthetic broad-spectrum antibiotic is widely utilized in veterinary medicine to treat animal diseases and promote livestock growth, it can inhibit bacterial DNA gyrase subunit A, thereby preventing bacterial DNA replication and exerting its antibacterial effect. However, excessive use of enrofloxacin poses significant risks to ecological balance and human health due to residual contamination. We have developed a novel ENR aptamer sensor based on the gold nanoparticles/aptamer (AuNPs-Apt) complexes, in which AuNPs were synthesized via the seed method and functionalized with aptamers. The optical properties, particle size, functional groups and morphology of the AuNPs-Apt probe were characterized by transmission electron microscope, Fourier transform infrared spectrometer and UV–vis spectrophotometer, respectively. The aptamer biosensor can specifically identify enrofloxacin, with a wide detection range (0.05–100 μg ml−1) and a good linear relationship ( R 2 =0.99) within the detection range. In addition, the biosensor also has the advantages of short detection time, low biological toxicity, good stability, and low detection cost. Therefore, it shows a great prospect for practical application in the field of detecting enrofloxacin residues.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Development of a rapid detection method for enrofloxacin in food

Cited by 2 publications

References 15 publications

Induction of the mitochondrial pathway of apoptosis by enrofloxacin in the context of the safety issue of its use in poultry

Induction of the mitochondrial pathway of apoptosis by enrofloxacin in the context of the safety issue of its use in poultry

A novel sensing probe based on AuNPs-Apt for the detection of enrofloxacin

Contact Info

Product

Resources

About