Development of a Rapid, Efficient, Intelligent and Cost-Saving Tool to Diagnose Pasteurella Multocida by Using Whole Genome Sequence and Genotypes of Pasteurella Multocida From Different Hosts

Peng, Zhong; Liu, Junyang; Liang, Wei; Wang, Fei; Wang, Li; Hua, Lin; Wang, Xiangru; Tan, Chen; Zhou, Rui; Chen, Huanchun; Wilson, Brenda A.; Wang, Jia; Wu, Bin

doi:10.21203/rs.3.rs-104569/v1

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“…The results were in contradict with the previous study, in which the inoculation of rabbit sourced P. multocida serogroup F isolate J-4103 caused high mortality and resulted in severe pathological lesions in most of the challenged rabbits (acute septicemic syndrome and extensive hemorrhage in subcutis in subcutaneously challenged rabbits, and fibrinopurulent pleuropneumonia and extensive hemorrhagic pneumonia in intranasally challenged rabbits) [ 12 ]. Moreover, P. multocida serogroup F isolates from chicken (C21724H3km7), turkey (P-4218) and pig (HN07) were also highly virulent to rabbit without prior adaption [ 23 , 24 ]. The results suggested that the variation in virulence among P. multocida serogroup F strains, and the s4 was a low virulent strain by comparison with the J-4103, C21724H3km7, P-4218 and HN07.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic and genomic characterisation of a rabbit sourced Pasteurella multocida serogroup F isolate s4

et al. 2022

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Background Pasteurella multocida is one of the most significant pathogens for a number of animals. In rabbits, the infection is generally associated with the P. multocida serogroups A and D, and the knowledge about the serogroup F is limited. In the present study, a P. multocida serogroup F isolate designated s4 was recovered from the lungs of rabbits died of respiratory disease in Fujian, in the southeast of China. The pathogenicity and genomic features of the s4 were then determined. Results The serotype and sequence type of s4 were F:L3 and ST12, respectively. The s4 was pathogenic for rabbits, but it was a low virulent strain comparing to the previously reported highly pathogenic P. multocida serogroup F strains J-4103, C21724H3km7, P-4218 and HN07. The whole genome of the s4 was then sequenced to understand the genomic basis for pathogenicity. Particularly, a large-sized fragment of approximate 275 kb in length was truncated from the chromosome to form a plasmid. Moreover, the in-frame deletion of natC and N-terminal redundance of gatF would resulted in the production of a mutant L3 outer core structure that was distinct from those of the other P. multocida strains belonging to the lipopolysaccharide genotype L3. We deduced that these features detected in the genome of s4 might impair the pathogenicity of the bacterium. Conclusions This study evaluated the pathogenicity and determined the genomic features of the rabbit sourced P. multocida serogroup F isolate s4, the observations and findings would helpful for the understanding of the pathogenicity variability and genetic diversity of P. multocida.

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Section: Discussionmentioning

confidence: 99%