Development of a rapid electrophoretic assay for genomic DNA damage quantification

Londero, James Eduardo Lago; Schavinski, Cassiano Ricardo; Silva, Fernanda D’Avila da; Piccoli, Bruna Candia; Schuch, André Passaglia

doi:10.1016/j.ecoenv.2020.111859

Cited by 4 publications

(1 citation statement)

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“…The currently available scanners and densitometers are not common equipment; their resolution is insufficient to work with a densitogram like with a chromatogram. Secondly, the existing software mostly specializes in electrophoresis of nucleic acids and therefore uses a different signal-to-noise ratio [33].…”

Section: Introductionmentioning

confidence: 99%

On the Applicability of Electrophoresis for Protein Quantification

et al. 2021

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Polyacrylamide gel electrophoresis is widely used for studying proteins and protein-containing objects. However, it is employed most frequently as a qualitative method rather than a quantitative one. This paper shows the feasibility of routine digital image acquisition and mathematical processing of electropherograms for protein quantification when using vertical gel electrophoresis and Chrom & Spec software. Both the well-studied model protein molecules (bovine serum albumin) and more complex real-world protein-based products (casein-containing isolate for sports nutrition), which were subjected to mechanical activation in a planetary ball mill to obtain samples characterized by different protein denaturation degrees, were used as study objects. Protein quantification in the mechanically activated samples was carried out. The degree of destruction of individual protein was shown to be higher compared to that of the protein-containing mixture after mechanical treatment for an identical amount of time. The methodological approach used in this study can serve as guidance for other researchers who would like to use electrophoresis for protein quantification both in individual form and in protein mixtures. The findings prove that photographic imaging of gels followed by mathematical data processing can be applied for analyzing the electrophoretic data as an affordable, convenient and quick tool.

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Section: Introductionmentioning

confidence: 99%