Development of a rat model for type 2 diabetes mellitus peri‐implantitis: A preliminary study

He, Qingqing; Mu, Zhixiang; Shrestha, Annie; Wang, Chao; Wang, Si; Tang, Han; Li, Yihan; Song, Jinlin; Ji, Ping; Huang, Yuanding; Chen, Tao

doi:10.1111/odi.13845

Cited by 12 publications

(16 citation statements)

References 62 publications

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“…While the machined surface implants were the most common (n=13), surface modifications were also employed (sandblasted and acid-etched surfaces [ 11 12 13 ], acid-etched surfaces [ 14 ], and hydroxyapatite-coated surfaces [ 15 ]). The implant surface properties were not reported in 6 studies [ 16 17 18 19 20 21 ].…”

Section: Resultsmentioning

confidence: 99%

Rodent peri-implantitis models: a systematic review and meta-analysis of morphological changes

Chew

Sim

et al. 2022

J Periodontal Implant Sci

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Purpose Rodent models have emerged as an alternative to established larger animal models for peri-implantitis research. However, the construct validity of rodent models is controversial due to a lack of consensus regarding their histological, morphological, and biochemical characteristics. This systematic review sought to validate rodent models by characterizing their morphological changes, particularly marginal bone loss (MBL), a hallmark of peri-implantitis. Methods This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. A literature search was performed electronically using MEDLINE (PubMed), and Embase, identifying pre-clinical studies reporting MBL after experimental peri-implantitis induction in rodents. Each study’s risk of bias was assessed using the Systematic Review Center for Laboratory animal Experimentation (SYRCLE) risk of bias tool. A meta-analysis was performed for the difference in MBL, comparing healthy implants to those with experimental peri-implantitis. Results Of the 1,014 unique records retrieved, 23 studies that met the eligibility criteria were included. Peri-implantitis was induced using 4 methods: ligatures, lipopolysaccharide, microbial infection, and titanium particles. Studies presented high to unclear risks of bias. During the osseointegration phase, 11.6% and 6.4%-11.3% of implants inserted in mice and rats, respectively, had failed to osseointegrate. Twelve studies were included in the meta-analysis of the linear MBL measured using micro-computed tomography. Following experimental peri-implantitis, the MBL was estimated to be 0.25 mm (95% confidence interval [CI], 0.14–0.36 mm) in mice and 0.26 mm (95% CI, 0.19–0.34 mm) in rats. The resulting peri-implant MBL was circumferential, consisting of supra- and infrabony components. Conclusions Experimental peri-implantitis in rodent models results in circumferential MBL, with morphology consistent with the clinical presentation of peri-implantitis. While rodent models are promising, there is still a need to further characterize their healing potentials, standardize experiment protocols, and improve the reporting of results and methodology. Trial Registration PROSPERO Identifier: CRD42020209776

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Section: Resultsmentioning

confidence: 99%

Rodent peri-implantitis models: a systematic review and meta-analysis of morphological changes

Chew

Sim

et al. 2022

J Periodontal Implant Sci

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“…However, DM, as a kind of metabolic disorder, has been reported to decrease bone mineral content and retard the process of implant fixation, leading to the implant failure . Previous reports have indicated that an overall reduction in bone turnover rather than excessive bone resorption contributed to DM-induced bone healing impair. , The abnormal osteoblast structure and biological functions at the bone–implant interface play a critical role in the above process . To correct the abnormality, improving metabolic control in DM which reverses impaired bone healing seems to be a good measure but is hardly achieved within a short time for urgent clinical surgery.…”

Section: Discussionmentioning

confidence: 99%

“…9,36 The abnormal osteoblast structure and biological functions at the bone−implant interface play a critical role in the above process. 37 To correct the abnormality, improving metabolic control in DM which reverses impaired bone healing seems to be a good measure 38 but is hardly achieved within a short time for urgent clinical surgery. Therefore, it is an urgent need to search for alternative valuable improvement strategies to promote rapid implant osseointegration in diabetics.…”

Section: Discussionmentioning

confidence: 99%

Silk Fibroin/Hydroxyapatite Coating Improved Osseointegration of Porous Titanium Implants under Diabetic Conditions via Activation of the PI3K/Akt Signaling Pathway

Cui

Wang

et al. 2022

ACS Biomater. Sci. Eng.

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The application of three-dimensional printed porous titanium implants (TIs) is compromised in patients suffering from diabetes mellitus (DM), which disturbs the normal process of implant osseointegration, resulting in fixation failure. It was possibly because of reactive oxygen species (ROS) overproduction at the bone−implant interface. A silk fibroin-based hydroxyapatite (SF/HA) hybrid material emerged as a novel biological material for accelerating new bone formation. We proposed that the SF/HA hybrid coated titanium implant (SHT) could mitigate DM-mediated impaired osseointegration, which had never been reported previously. To test this assumption and further elucidate the mechanisms, primary rabbit osteoblasts were seeded on TIs or SHTs and cultured with normal serum, diabetic serum (DS), DS + N-acetyl-L-cysteine (NAC) (a potent ROS inhibitor), and DS + LY294002 (a specific PI3K/Akt inhibitor) for osteoblast behavior examinations. An animal study was performed on diabetic rabbits implanted with the two kinds of implants for osseointegration tests. DM-mediated ROS overproduction caused osteoblastic biological dysfunctions and apoptotic injury, associated with suppression of PI3K/Akt signaling in osteoblasts cultured on a TI substrate. Of note, the SHT substrate significantly suppressed ROS overproduction under diabetic conditions, improved osteoblast functional recovery including ameliorative osteoblast adhesion and morphology, improved cellular proliferation and differentiation, and abrogated apoptosis, which exhibited the same effect as NAC administration on the TI. The in vitro results were further corroborated in vivo by enhanced osteogenesis and osseointegration of SHTs in diabetic rabbits. Moreover, the aforesaid promotive effects afforded by the SF/HA coating were totally abolished with administration of LY294002 for blocking PI3K/Akt signaling. The above results collectively demonstrated that the SF/HA hybrid coating significantly ameliorated DM-mediated impaired osseointegration of the TI via reactivation of the ROSmediated PI3K/Akt signaling pathway. The hybrid coating elicited a novel surface biofunctionalization strategy to attain favorable clinical performance of TI in diabetics.

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“…This work was to understand the effect of n-HA/PA66 material prepared in this experiment on bone defect healing of diabetic implants. Alloxan can selectively destroy pancreatic β cells, cause cell failure, block cell secretion of insulin, and then cause diabetes [ 27 ]. The results showed that the weight of experimental rabbits was considerably inferior to that of normal rabbits, while the blood glucose level was always higher than that of normal rabbits.…”

Section: Discussionmentioning

confidence: 99%

[Retracted] Efficiency of Nanohydroxyapatite on Repairing Type II Diabetes Dental Implant‐Bone Defect

2022

BioMed Research International

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The purpose of this study was to see how a nanohydroxyapatite (n-HA) composite polyamide 66 (PA66) affected the repair of bone defects in diabetics with titanium implants, as well as to develop experimental materials for the creation of the interface between bone tissue and titanium implants. Rabbit bone marrow mesenchymal stem cells (MSCs) were isolated using n-HA/PA66 composite material, and the effect of coculture with the material on cell proliferation was analyzed after induction of mineralization. Bone defect models of diabetic experimental rabbits and titanium implants were prepared. Normal rabbits with bone defects were used as control (NC group, N = 8 ). After the diabetic bone defect (DM group, N = 8 ) and the implantation of n-HA/PA66 composite material (n-HA/PA66 group, N = 8 ), the differences in body weight, blood glucose, scanning electron microscopy of the implant-bone interface, bone mineral density, new bone trabecular parameters, histomorphology, and biomechanical properties of the implant-bone interface were compared and analyzed. In vitro test results showed that MSC cell growth could be promoted by mineralization induction, the cell growth condition was good after coculture with n-HA/PA66, and the proliferation activity of MSCs was not affected by the material. In vivo test results showed that the body weight of the DM group and n-HA/PA66 group was considerably inferior to that of the NC group, and the blood glucose was dramatically superior to that of the NC group ( P < 0.05 ). However, the body weight of the n-HA/PA66 group was dramatically superior to that of the DM group ( P < 0.05 ). The bone mineral density, bone volume fraction (BV/TV), bone surface area fraction (BS/BV), bone trabecular thickness (Tb.Th), bone trabecular number (Tb.N), bone trabecular area, and biomechanical properties in the DM group were considerably inferior to those in the NC group and n-HA/PA66 group ( P < 0.05 ). The trabecular space (Tb.Sp) in the NC group and n-HA/PA66 group was dramatically superior to that in the NC group ( P < 0.05 ). The bone mineral density, BV/TV, BS/BV, Tb.Th, Tb.N, trabecular area, and biomechanical properties of the n-HA/PA66 group were dramatically superior to those of the NC group ( P < 0.05 ), while Tb.Sp was considerably inferior to that of the NC group ( P < 0.05 ). These findings showed that the n-HA/PA66 material had good biocompatibility and minimal cytotoxicity, and that filling the space between the surrounding bone and the titanium implant can enhance bone repair. This research paved the way for future research into the tissue-engineered bone in the field of oral surgery.

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Development of a rat model for type 2 diabetes mellitus peri‐implantitis: A preliminary study

Cited by 12 publications

References 62 publications

Rodent peri-implantitis models: a systematic review and meta-analysis of morphological changes

Rodent peri-implantitis models: a systematic review and meta-analysis of morphological changes

Silk Fibroin/Hydroxyapatite Coating Improved Osseointegration of Porous Titanium Implants under Diabetic Conditions via Activation of the PI3K/Akt Signaling Pathway

[Retracted] Efficiency of Nanohydroxyapatite on Repairing Type II Diabetes Dental Implant‐Bone Defect

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