Development of a real-time PCR method for the simultaneous detection of soya and lupin mitochondrial DNA as markers for the presence of allergens in processed food

Galán, Antonio Maiques; Brohée, Marcel; Andrade, Eugénia de; Hengel, Arjon J. van; Chassaigne, Hubert

doi:10.1016/j.foodchem.2011.01.019

Cited by 37 publications

(18 citation statements)

References 34 publications

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“…Immunological methods were enzyme-linked immunosorbent assay (ELISA) (Fuller, Goodwin, & Morris, 2006;Zhou et al, 2013), rocket immunoelectrophoresis (Moen et al, 2005), and immunochromatographic strip tests (Puerta, Diez-Masa, & de Frutos, 2006;Puerta, Jaulmes, De Frutos, Diez-Masa, & Vidal-Madjar, 2002). Assays based on DNA analysis were mainly polymerase reaction (PCR) and fluorescence quantitative PCR (Galan, Brohee, Silva, van Hengel, & Chassaigne, 2011). Although PCR was highly sensitive and stable, it was not applicable for all allergens detection because of a relatively high false-positive.…”

Section: Introductionmentioning

confidence: 99%

Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk

Chao

et al. 2017

Food and Agricultural Immunology

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In this work, a goldmag-based enzyme-linked immunosorbent assay was developed for determination of α-lactalbumin (α-LA) in milk. The magnetic nanoparticle functionalized with polyetherimide was synthesized by one-pot method and coated with two layers of gold nanoparticles on the surface to synthesize goldmag nanoparticles. Anti-α-LA monoclonal antibody, prepared by hybridoma cell lines via cell fusion, was then bound to this goldmag nanoparticle to develop a capture nanoprobe. The results showed that this developed immunoassay had a good linear range of 2.33-127.1 ng/mL with IC 50 of 17.2 ng/mL. Besides, recovery rates for α-LA in four commercial milks were from 86.7% to 109.8%. The coefficients of variation in intra-assay and interassay were 3.9-6.8% and 5.5-9.8%, separately, which could meet the requirements to quantify α-LA content in milk. This goldmagbased immunoassay might have considerable potentials in the detection of food allergies. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk

Chao

et al. 2017

Food and Agricultural Immunology

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“…The most important feature of mtDNA is that it can be found in higher numbers in cells compared to that of nuclear DNA. Therefore, using mtDNA as a target would allow much easier amplification even if the raccoon dog meat component is present in the lowest concentration among other types of meat in a meat mixture [21,22]. Therefore, to increase sensitivity of detection, in our study, mtDNA was chosen as a target sequence.…”

Section: Discussionmentioning

confidence: 99%

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures

Liu

Shi²,

Teng

et al. 2017

Journal of Food Quality

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Raccoon dog (Nyctereutes procyonoides) is an economically important animal used for fur production, but consuming its meat is injurious to human health. Currently, no rapid and sensitive method for detecting raccoon dog meat in meat mixtures is available. In this study, we developed an easily applicable, rapid, and economically feasible method for identifying the presence of raccoon dog in meat mixtures based on loop-mediated isothermal amplification (LAMP). Four sets of LAMP primers were tested at different temperatures, and the primers that worked best at 62°C (set 2) were determined. In the LAMP assay, there was no cross-reactivity with the meat procured from other species of animals and the detection limit of DNA concentration was 0.1 pg·μL−1, slightly higher than TaqMan real-time PCR (0.01 pg·μL−1), but sensitivity of 0.1 pg·μL−1 complies with most requirements of routine analysis. Moreover, by the LAMP method, the meat mixtures containing more than 0.5% of the raccoon dog component were directly detected (without DNA extraction) in the supernatant isolated from the meat mixtures after performing repeated cycles of thawing and freezing of minced meat mixtures. Our results show that LAMP assay is a valuable, straightforward, and sensitive detection tool for identification of raccoon dog meat in mixtures.

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“…Duplex real-time PCR method was used to simultaneously detect traces of lupin and soya in processed food. Both lupin and soya at a level of 2.5 mg/kg food matrix could be detected in cookies (Galan et al, 2011). Koeppel et al (2010) developed two novel quantitative multiplex real-time PCR systems.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Soybean Allergens: Presence, Detection and Methods for Mitigation

Yang¹,

Mejı́a²,

Zheng³

et al. 2011

Soybean and Health

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Development of a real-time PCR method for the simultaneous detection of soya and lupin mitochondrial DNA as markers for the presence of allergens in processed food

Cited by 37 publications

References 34 publications

Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk

Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures

Soybean Allergens: Presence, Detection and Methods for Mitigation

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