Development of a red-light emission hypoxia-sensitive two-photon fluorescent probe for <i>in vivo</i> nitroreductase imaging

Gebremedhin, Kalayou H.; Li, Yaming; Yao, Qichao; Xiao, Ming; Gao, Fengli; Fan, Jiangli; Du, Jianjun; Long, Saran; Peng, Xiaojun

doi:10.1039/c8tb02635a

Cited by 50 publications

(25 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prior to cell imaging experiments, the cytotoxicities of the nanoprobe NP-SPT-NO 2 against 4T1 cell line (murine mammary carcinomac ell line overexpressing NTR under hypoxia [30] )a nd LO2 cell line (normal human hepatic cell line which has no overexpression of NTR) were evaluated ( Figure S24), and the result confirmed that the nanoprobe presents little cytotoxicity. The intracellular imaging of NTR by NP-SPT-NO 2 was investigated in 4T1 cells by fluorescencei maging.…”

Section: Resultsmentioning

confidence: 80%

“…In addition, optoacoustic (OA) imaging for NTR in 4T1 cells by the nanoprobe are shown in Figure S26. Clearly, after incubation with NP-SPT-NO 2 under hypoxia, strong OA signals as ar esult of irradiation at 690 nm could be observed in 4T1 cancerc ells, [30] whereas the OA signals (irradiated at 690 nm) were insignificant in the cells under normoxia. These results indicate that the NP-SPT-NO 2 can respond to thei ntracellular NTR, thereby producings trong fluorescent and optoacousticsignals.…”

Section: Resultsmentioning

confidence: 92%

See 1 more Smart Citation

An Unsymmetrical Squaraine‐Based Activatable Probe for Imaging Lymphatic Metastasis by Responding to Tumor Hypoxia with MSOT and Aggregation‐Enhanced Fluorescent Imaging

Lin

Sun

Zeng

et al. 2019

Chemistry A European J

View full text Add to dashboard Cite

Optoacoustic imaging has great potential for preclinicalr esearch andc linical practice, and designing robust activatable optoacoustic probes for specific diseases is beneficial for its further development. Herein, an activatable probe has been developed for tumor hypoxia imaging. For this probe,i ndole and quinoline were linked on each side of an oxocyclobutenolate core to form an unsymmetrical squaraine. At riarylamine group was incorporated to endow the molecule with the aggregation enhanced emission (AEE) properties. In aqueousm edia, the squaraine chromophore aggregates into the nanoprobe, which specifically responds to nitroreductase andp roduces strong optoacoustics ignals due to its high extinction coefficient, as well as prominent fluorescencee mission as ar esult of its AEE feature. The nanoprobe was used to image tumorm etastasis via the lymphatic system both optoacoustically and fluorescently.M oreover,b oth the fluorescences ignals andt hree-dimensional multispectral optoacoustic tomography signals from the activatedn anoprobe allow us to locatet he tumor site and to map the metastatic route.

show abstract

Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 92%

An Unsymmetrical Squaraine‐Based Activatable Probe for Imaging Lymphatic Metastasis by Responding to Tumor Hypoxia with MSOT and Aggregation‐Enhanced Fluorescent Imaging

Lin

Sun

Zeng

et al. 2019

Chemistry A European J

View full text Add to dashboard Cite

show abstract

“…As displayed in Figure S27, NP‐Q‐NO 2 shows low cytotoxicity against 4T1 cells. Afterwards, the nanoprobe was employed to image endogenous nitroreductase in 4T1 cells (the murine mammary carcinoma cell line, which is known to overexpress nitroreductase under hypoxia) . From Figure S28, one can see that the cells incubated with the nanoprobe under hypoxic conditions for 0.5 h exhibited evident fluorescence, either under the excitation of 675 nm LED light or 808 nm laser light (at 50 mW cm −2 ).…”

Section: Resultsmentioning

confidence: 99%

Nanoaggregate Probe for Breast Cancer Metastasis through Multispectral Optoacoustic Tomography and Aggregation‐Induced NIR‐I/II Fluorescence Imaging

et al. 2019

View full text Add to dashboard Cite

An activatable nanoprobe for imaging breast cancer metastases through near infrared‐I (NIR‐I)/NIR‐II fluorescence imaging and multispectral optoacoustic tomography (MSOT) imaging was designed. With a dihydroxanthene moiety serving as the electron donor, quinolinium as the electron acceptor and nitrobenzyloxydiphenylamino as the recognition element, the probe can specifically respond to nitroreductase and transform into an activated D‐π‐A structure with a NIR emission band extending beyond 900 nm. The activated nanoprobe exhibits NIR emission enhanced by aggregation‐induced emission (AIE) and produces strong optoacoustic signal. The nanoprobe was used to detect and image metastases from the orthotopic breast tumors to lymph nodes and then to lung in two breast cancer mouse models. Moreover, the nanoprobe can monitor the treatment efficacy during chemotherapeutic course through fluorescence and MSOT imaging.

show abstract

“…Nitroreductase (NTR) is an endogenous enzyme that overexpressed in hypoxic tumors, and the concentration of NTR is directly concerned to the degree of hypoxia. [10] NTR is seen as an indicator of highly invasive diseases in various hypoxic tumors and NTR overexpression plays important roles in tumor invasion, progression and angiogenesis [11]. Further studies show that the hypoxic status of solid tumors is closely related to the process of tumor progression [12].…”

Section: Introductionmentioning

confidence: 99%

Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Wang

Zhang

Huang

et al. 2020

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Tumor resistance is a huge challenge for tumor treatment, which may lead to tumor treatment failure. Relieving tumor resistance and improving efficacy are long-term goals for tumor treatment. Nitroreductase (NTR) is an endogenous enzyme that highly expressed in hypoxia tumors. Herein, we develop a NTR-activatable fluorescent probe, TP-NO 2 , for NTR detection during tumor treatment. TP-NO 2 with simple synthesis steps and high yield can qualitatively and quantitatively detect NTR in a wide range (0−20 μg/mL) with a detection limit of 26 ng/ mL. The probe has been successfully applied for NTR detection in cells under simulated hypoxia conditions. Hyperbaric oxygen (HBO) can alleviate tumor hypoxia and reduce NTR concentration. We pre-evaluate the effect of tumor treatment by NTR imaging. And the results demonstrate that HBO-assistant chemotherapy can effectively treat tumor cells. We further apply TP-NO 2 for NTR detection in A549 and cis-dichlorodiamineplatinum(II) (DDP)-resistant A549 (A549/DDP) xenograft nude mice, and we choose NTR as an indicator to pre-evaluate the treatment efficacy of malignant tumors. With the adjuvant therapy of HBO, chemotherapeutic drugs gemcitabine and carboplatin can effectively treat A549 and A549/DDP xenograft. These applications provide us a novel perspective for NTR imaging.

show abstract

Development of a red-light emission hypoxia-sensitive two-photon fluorescent probe for in vivo nitroreductase imaging

Abstract: A red-light emission nitroreductase-activatable two-photon fluorescent probe, namely NRP, was developed for monitoring of degree of hypoxia in cells and in vivo nitroreductase imaging.

Cited by 50 publications

References 41 publications

An Unsymmetrical Squaraine‐Based Activatable Probe for Imaging Lymphatic Metastasis by Responding to Tumor Hypoxia with MSOT and Aggregation‐Enhanced Fluorescent Imaging

An Unsymmetrical Squaraine‐Based Activatable Probe for Imaging Lymphatic Metastasis by Responding to Tumor Hypoxia with MSOT and Aggregation‐Enhanced Fluorescent Imaging

Nanoaggregate Probe for Breast Cancer Metastasis through Multispectral Optoacoustic Tomography and Aggregation‐Induced NIR‐I/II Fluorescence Imaging

Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Contact Info

Product

Resources

About