Development of a regeneration system via nodal segment culture inVeronica anagallis-aquaticaL. – an amphibious medicinal plant

Abstract

The present study describes an effective, reproducible and rapid regeneration protocol for in vitro shoot development of the medicinally important, semi-aquatic plant Veronica anagallis-aquatica L. through mature nodal segments on Murashige and Skoog (MS) medium. The most effective medium for shoot regeneration was MS medium fortified with 0.5 mM 6-benzyladenine (BA), on which the maximum number (43.791.85) of shoots per explant having a shoot length of 5.090.25 cm were produced. The addition of auxins along with optimal concentration (0.5 mM) of BA did not further improve the regeneration capacity of the explants; instead, it resulted in an adverse effect. Nodal segments cultured in MS liquid medium without agar provided a better and early response for efficient shoot regeneration, and simultaneous rooting as compared to solidified medium. The optimal liquid medium comprised of MS'BA (0.5 mM) on which a maximum 20.791.76 shoots per explant were produced and grew healthier. Both half strength MS and MS basal medium supplemented with anaphthaleneacetic acid (NAA) responded equally for root induction. Rooted shoots were successfully transferred to field conditions with 80% survival. Histological studies revealed the de novo origin of shoots which would make this protocol suitable for transformation techniques.

In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

“…Increasing the concentration of BAP in the induction medium decreased the length of shoots ( Figure 2 A, and data not shown). A similar inhibitory effect of BAP beyond its optimum level on the axillary bud induction frequency, number of shoots per explants, and average shoot length has been well-documented in other plants [ 11 , 20 , 46 , 47 , 49 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ]. A possible reason for the reduction in the regeneration potential was a detrimental effect of BAP at higher concentrations [ 58 , 59 , 60 ] or a formation of an excessive basal callus at the expense of shoot proliferation [ 12 , 56 ].…”

Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?

“…Previously, several studies reported that the auxin and cytokinin ratio in the media produced shoot formation using different explant sources and genotypes in Ocimum spp. (Shahzad, Parveen, & Fatema, 2011) whereas, in the present study, the combination of two cytokinins (BAP + KIN) was more effective in the induction of the maximum number of shoots (Supplementary Table S1). …”

Factors affecting in vitro plant regeneration from cotyledonary node explant of Senna sophera (L.) Roxb. A highly medicinal legume

“…Beyond the optimal concentration (7.5 µM), a decrease in the response as well as number of shoots was recorded due to profuse basal callusing (Table 2). Similarly, the superiority of BA on bud break and multiple shoot production has also been reported earlier in several medicinal and aromatic plants including Mucuna pruriense (Sathyanarayana et al, 2008); Clitoria ternatea (Singh and Tiwari, 2010); Justicia gendarussa (Thomas and Yoichiro, 2010) and Veronica anagallis-aquatica (Shahzad et al, 2011).…”

“…Advances in biotechnology, especially in vitro techniques provide an important tool for multiplication, conservation and propagation of several medicinal plants ( Khallafalla and Daffalla, 2008;Shahzad et al, 2011;Li et al, 2012). Till date there is a single report on micropropagation of S. sophera (Parveen and Shahzad, 2010).…”