Development of a safe and efficient gene delivery system based on a biodegradable tannic acid backbone

High-Axial-Aspect Tannic Acid Microparticles Facilitate Gelation and Injectability of Collagen-Based Hydrogels

“…Found in different parts of plants (leaves, bark, and roots), beverages (tea and coffee), fruits (grapes), and vegetables (mint and basil), 26 TA is a polyphenolic compound that imparts various therapeutic characteristics (antioxidant, antimicrobial, anti-inflammatory, and antimutagenic) if applied at preferred locations. 27−29 Due to its cross-linking ability through the presence of multiple hydroxyl and carboxylic groups 30,31 and beneficial functionalities, TA has been employed in a variety of applications from drug delivery and wound healing to 3D printing. 32−34 Recent efforts have been made in fabricating scaffolds with collagen, cross-linked with different concentrations of TA either by cross-mixing or by soaking the scaffold in TA solution.…”

“…The use of tannic acid (TA) in tandem with collagen offers a unique opportunity to tailor the gel’s rheological properties while adding functionality. Found in different parts of plants (leaves, bark, and roots), beverages (tea and coffee), fruits (grapes), and vegetables (mint and basil), TA is a polyphenolic compound that imparts various therapeutic characteristics (antioxidant, antimicrobial, anti-inflammatory, and antimutagenic) if applied at preferred locations. − Due to its cross-linking ability through the presence of multiple hydroxyl and carboxylic groups , and beneficial functionalities, TA has been employed in a variety of applications from drug delivery and wound healing to 3D printing. − Recent efforts have been made in fabricating scaffolds with collagen, cross-linked with different concentrations of TA either by cross-mixing or by soaking the scaffold in TA solution. − Injectable collagen/TA beads have been developed by immersing collagen beads in TA solution before injection, where inclusion of TA imparts the anticancer property to the injectables. , However, to the best of our knowledge, a detailed understanding of the rheological behavior of collagen/TA-based hydrogels, critical in all these applications and, in particular, the sol–gel and time-dependent aspect of the injection procedure, remains unexplored. In this study, we focus on understanding the rheology of collagen systems incorporating TA microparticles.…”

Effect of molecular weight of polysaccharide on efficient plasmid DNA delivery by polyethylenimine‐polysaccharide‐Fe(III) complexes

“…Polysaccharides compile a large variable family of heterogenic sequences of monomers due to the multiple valences of monomers and the stereo specificity of the glycosidic bonds 29,30 . Many kinds of polysaccharides were used to modify PEI to obtain safe and efficient gene vector, because polysaccharides has several advantages, such as availability from replenishable agricultural or marine food resources, biocompatibility and biodegradability 31–33 . Rice bran polysaccharide (RBP) can be obtained from rice bran by aqueous extraction and is not only biocompatible but also abundant in nature.…”

“…29,30 Many kinds of polysaccharides were used to modify PEI to obtain safe and efficient gene vector, because polysaccharides has several advantages, such as availability from replenishable agricultural or marine food resources, biocompatibility and biodegradability. [31][32][33] Rice bran polysaccharide (RBP) can be obtained from rice bran by aqueous extraction and is not only biocompatible but also abundant in nature. Modification of PEI by RBP can effectively reduce the cytotoxicity of PEI and obtain higher transfection efficiency.…”

Preparation of PEI‐modified nanoparticles by dopamine self‐polymerization for efficient DNA delivery

“…27,28 PEI is rich in amino groups, easily combined with other biocompatible molecules and prepared such as polyethylene glycol (PEG)-modified PEI, polysaccharidemodified PEI, tannic acid-modified PEI copolymer, and shows higher safety and transfection efficiency. [29][30][31] In addition, by covalently binding with targeting molecules, such as RGD peptide and folic acid (FA), the targeting ability of PEI as a gene carrier can be significantly improved. 32,33 In this study, DA self-polymerization was used to form a PDA layer to coat Fe 3 O 4 NPs, and then the surface functionalized NPs (named Fe 3 O 4 @PDA@PEI) were prepared by covalent binding between PDA and amino groups of PEI (Figure 1).…”