Development of a sandwich ELISA for the detection of Chinese sacbrood virus infection

Li, Ming; Sun, Li; Ma, Yueyu; Fei, Dongliang; Ma, Mai‐Juan

doi:10.1007/s00705-020-04634-2

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…The HDA amplification procedure was conducted at 65 • C within 60 min, showing a high specificity (4 pg RNA) compared with 40 pg RNA in the RT-PCR procedure. In addition, the HDA method showed a high sensitivity, specificity, and stability in detecting the Chinese sacbrood virus (CSBV) with an LOD of 10 −2 ng [101]. The optimal reaction conditions were 65 • C, 90 min, and 5 µmol/L of primer.…”

Section: Helicase-dependent Amplificationmentioning

confidence: 99%

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Ngoc,

Lee

2024

Biosensors

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Ribonucleic acid (RNA) viruses are one of the major classes of pathogens that cause human diseases. The conventional method to detect RNA viruses is real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), but it has some limitations. It is expensive and time-consuming, with infrastructure and trained personnel requirements. Its high throughput requires sophisticated automation and large-scale infrastructure. Isothermal amplification methods have been explored as an alternative to address these challenges. These methods are rapid, user-friendly, low-cost, can be performed in less specialized settings, and are highly accurate for detecting RNA viruses. Microfluidic technology provides an ideal platform for performing virus diagnostic tests, including sample preparation, immunoassays, and nucleic acid-based assays. Among these techniques, nucleic acid isothermal amplification methods have been widely integrated with microfluidic platforms for RNA virus detection owing to their simplicity, sensitivity, selectivity, and short analysis time. This review summarizes some common isothermal amplification methods for RNA viruses. It also describes commercialized devices and kits that use isothermal amplification techniques for SARS-CoV-2 detection. Furthermore, the most recent applications of isothermal amplification-based microfluidic platforms for RNA virus detection are discussed in this article.

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Section: Helicase-dependent Amplificationmentioning

confidence: 99%

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Ngoc,

Lee

2024

Biosensors

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“…Techniques developed for detecting viruses in honey bees are based on various approaches including enzyme-linked immunosorbent assay (ELISA), oligonucleotide microarray, cell lines, quantitative PCR (qPCR and RT-qPCR) and metagenomic next generation sequencing (mNGS) [25][26][27][28][29][30][31][32]. PCR remains one of the most favored and applied techniques for virus detection and disease diagnosis.…”

Section: Introductionmentioning

confidence: 99%

A semi-automated and high-throughput approach for the detection of honey bee viruses in bee samples

Nikulin,

Hesketh-Best,

Mckeown

et al. 2024

PLoS ONE

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Deformed wing virus (DWV) was first detected in dead honey bees in 1982 but has been in honey bees for at least 300 years. Due to its high prevalence and virulence, they have been linked with the ongoing decline in honey bee populations worldwide. A rapid, simple, semi-automated, high-throughput, and cost-effective method of screening colonies for viruses would benefit bee research and the beekeeping industry. Here we describe a semi-automated approach that combines an RNA-grade liquid homogenizer followed by magnetic bead capture for total virus nucleic acid extraction. We compare it to the more commonly applied nucleic acid column-based purification method and use qPCR plus Oxford Nanopore Technologies sequencing to evaluate the accuracy of analytical results for both methods. Our results showed high reproducibility and accuracy for both approaches. The semi-automated method described here allows for faster screening of viral loads in units of 96 samples at a time. We developed this method to monitor viral loads in honey bee colonies, but it could be easily applied for any PCR or genomic-based screening assays.

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Current methods and prospects of coronavirus detection

Deng

Liu

et al. 2021

Talanta

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Development of a sandwich ELISA for the detection of Chinese sacbrood virus infection

Cited by 6 publications

References 14 publications

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

A semi-automated and high-throughput approach for the detection of honey bee viruses in bee samples

Current methods and prospects of coronavirus detection

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