Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki)

Chae, Chi Won; Yun, Su Hyun; Park, Jae Ho; Lee, Dong Hoon

doi:10.5352/jls.2011.21.12.1659

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…Chae et al reported the development of DNA marker linked to male fertility of C . sunki [ 29 ]. Quantitative trait locus (QTL) analysis of the reduced pollen production trait will clarify the number of genes likely to be involved in regulation of the trait.…”

Section: Resultsmentioning

confidence: 99%

Segregation and Heritability of Male Sterility in Populations Derived from Progeny of Satsuma Mandarin

et al. 2016

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Male sterility derived from Satsuma mandarin (Citrus unshiu) has been used in Japanese citrus breeding programs to obtain seedless cultivars, which is a desirable trait for consumers. Male sterility has often been evaluated by anther development or pollen fertility; however, the inheritance and heritability of male sterility derived from Satsuma is poorly understood. In this study, we investigated the mode of inheritance and broad-sense heritability of male sterility derived from Satsuma. Initially, we evaluated the total number of pollen grains per anther and apparent pollen fertility, as indicated by lactophenol blue staining, in 15 citrus cultivars and selections to understand the male sterility of Satsuma. The results indicated that male sterility was primarily caused by decreased number of pollen grains per anther in progeny of Satsuma. We also evaluated these traits in three F1 populations (hyuganatsu × ‘Okitsu No. 56’, ‘Okitsu No. 46’ × ‘Okitsu No. 56’ and ‘Okitsu No. 46’ × ‘Kara’), of which the parents are derived from Satsuma. Individuals in these populations showed strong segregation for number of pollen grains per anther. The apparent fertility of pollen also showed segregation but was almost constant at 70%–90%. The estimated broad-sense heritability for the number of pollen grains per anther was as high as 0.898 in the ‘Okitsu No. 46’ × ‘Okitsu No. 56’ and ‘Okitsu No. 46’ × ‘Kara’ populations. These results indicated that the number of pollen grains per anther primarily determined male sterility among progeny of Satsuma, and this trait was inherited by the progeny. Development of DNA markers closely linked to male sterility using the F1 populations of ‘Okitsu No. 46’ × ‘Okitsu No. 56’ and ‘Okitsu No. 46’ × ‘Kara’ is expected to contribute to the breeding of novel seedless citrus cultivars.

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Section: Resultsmentioning

confidence: 99%

Segregation and Heritability of Male Sterility in Populations Derived from Progeny of Satsuma Mandarin

et al. 2016

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“…Yamamoto et al [ 8 ] demonstrate the interaction between nuclear and cytoplasmic genes by reciprocal hybridizations. Subsequent research has pointed to the involvement of nuclear genes in male sterility [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. DNA marker analysis for nuclear and cytoplasmic genomes and genome-wide SNP marker analysis showed that CMS in the satsuma was derived from its seed parent, the ‘Kishu’ mandarin ( C. kinokuni ), and that the nuclear genes come from the male parent, ‘Kunenbo’ mandarin ( C. nobilis ) [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus

Montalt

Cuenca²,

Vives

et al. 2023

Plants

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Polyembryony and male sterility (MS) are essential characters for citrus breeding. MS, coupled with parthenocarpy, allows for addressing the diversification of diploid seedless mandarin varieties, and nucleocytoplasmic MS is the most prevalent system. Polyembryony limits the use of seed parents in scion breeding programs, and the recovery of monoembryonic hybrids to be used as female parents is a crucial pre-breeding component. The objectives of this work were the identification of SNPs closely linked with the genes implied in these traits for marker-assisted selection. Genotyping by sequencing was used to genotype 61 diploid hybrids from an F1 progeny recovered from crossing ‘Kiyomi’ and ‘Murcott’ tangors. A total of 6444 segregating markers were identified and used to establish the two parental genetic maps. They consisted of 1374 and 697 markers encompassing 1416.287 and 1339.735 cM for ‘Kiyomi’ and ‘Murcott’, respectively. Phenotyping for MS and polyembryony was performed. The genotype–trait association study identified a genomic region on LG8 which was significantly associated with MS, and a genomic region on LG1 which was significantly associated with polyembryony. Annotation of the identified region for MS revealed 19 candidate genes. One SNP KASPar marker was developed and fully validated for each trait.

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“…Yamamoto et al (1997) demonstrate the interaction between nuclear and cytoplasmic genes by reciprocal hybridizations. Subsequent research has pointed to the involvement of nuclear genes in male sterility (Chae et al, 2011;Dewi et al, 2013bDewi et al, , 2013aNakano et al, 2001;. DNA marker analysis for nuclear and cytoplasmic genomes and genome-wide SNP marker analysis showed that CMS in the satsuma was derived from its seed parent, the 'Kishu' mandarin (C. kinokuni), and that the nuclear genes come from the male parent, 'Kunenbo' mandarin (C. nobilis) .…”

Section: Introductionmentioning

confidence: 99%

Assessment of Citrus Reproductive Biology for Seedless Mandarin Production and its Interaction with Temperature

Resurrección¹

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Since the origin of citrus, ancestral natural hybridizations, thousands of years of cultivation and admixture phenomenon have resulted in the complexity of the reproductive biology of today's cultivated citrus, which often involves female and male sterility, self-incompatibility, parthenocarpy and apomixis in different degrees. The global objective of this PhD was to increase our knowledge on different reproductive aspects that are crucial for citrus breeding and propagation and their interaction with environmental conditions.

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Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki)

Cited by 7 publications

References 34 publications

Segregation and Heritability of Male Sterility in Populations Derived from Progeny of Satsuma Mandarin

Segregation and Heritability of Male Sterility in Populations Derived from Progeny of Satsuma Mandarin

Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus

Assessment of Citrus Reproductive Biology for Seedless Mandarin Production and its Interaction with Temperature

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