Development of a Seeded Scaffold in the Great Omentum: Feasibility of an in vivo Bioreactor for Bladder Tissue Engineering

Baumert, H.; Simon, Patricia; Hekmati, Mehrak; Fromont, G; Lévy, M.; Balaton, A.; Molinié, Vincent; Malavaud, Bernard

doi:10.1016/j.eururo.2006.11.044

Cited by 83 publications

(45 citation statements)

References 20 publications

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“…Ses domaines d'application sont largement utilisés en ingénierie tissulaire pour la construction d'organes tels que la trachée [109], l'oesophage [67], la vessie [110] et l'intestin [111]. Sa position anatomique intra-abdominale permet de recevoir le substitut et de le transposer aisément par des techniques chirurgicales reproductibles [112].…”

Section: Méthodesunclassified

Esophageal tissue engineering

Luc

Durand

Collet

et al. 2014

Expert Review of Medical Devices

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Decellularized matrixes (DM) are commonly used to facilitate a constructive remodeling response in several types of tissue, including the esophagus. Surgical procedure of the esophagus is often complicated by stricture, but preclinical studies have shown that the use of a DM can mitigate stricture and promote a constructive outcome.Recognizing the potential benefits of DM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo remodeling properties of DM from porcine esophagus.The developed protocol for esophageal DM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a good mechanical behavior. Stem cells remained viable when seeded upon the esophageal DM in vitro, and the in-vivo host response showed a pattern of constructive remodeling when implanted in soft tissue.

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Section: Méthodesunclassified

Esophageal tissue engineering

Luc

Durand

Collet

et al. 2014

Expert Review of Medical Devices

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“…tissutale (3)(4)(5). La rigenerazione della vescica è una promettente applicazione delle metodiche di ingegneria tissutale e potrebbe in futuro essere utilizzata in Centri selezionati di 3° livello.…”

Section: Fig 4 -La Componente Muscolare Della Neovescica Ingegnerizzunclassified

“…In sintesi, abbiamo dimostrato nel nostro modello sperimentale che la rigenerazione della vescica così come di altri segmenti del tratto urinario, per mezzo di tecniche d'ingegneria tissutale, è oggi possibile nei modelli animali, ma è da considerare una metodica ancora sperimentale nell'uomo (3,4,12). Il processo di rigenerazione inizia dall'epitelio transizionale più interno, a contatto con le urine, e prosegue con l'invasione centripeta di fascetti muscolari lisci e di fibroblasti, con neo-angiogenesi e neo-tronchi nervosi in una matrice di collagene.…”

Section: Fig 4 -La Componente Muscolare Della Neovescica Ingegnerizzunclassified

Bladder Bioengineering: Our Experience

et al. 2012

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“…Bioreactors should be introduced in tissue engineering to optimize, through fluid shear, oxygenation and the supply of nutrients, the growth of cells on a 3D scaffold. This approach has been shown to result in better tissue-like constructs than do conventional static culture conditions (21). It is possible to use in vivo graft sites as 'bioreactors' that feature flowing fluids (blood).…”

Section: Bioreactormentioning

confidence: 99%

“…The authors believe that treated scaffold might be more suitable for bladder tissue engineering reconstruction. In order to enhance the vascularization of tissue engineered bladder scaffold in vitro, Baumert et al transferred the compound matrix into the greater omentum, which has been mentioned above (21).…”

Section: Animal Experimentsmentioning

confidence: 99%