Development of a sensitive and selective method for the quantitative analysis of cortisol, cortisone, prednisolone and prednisone in human plasma

Ionita, Ileana A.; Fast, Douglas M.; Akhlaghi, Fatemeh

doi:10.1016/j.jchromb.2009.02.019

Cited by 71 publications

(52 citation statements)

References 24 publications

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“…In contrast to routinely used immunoassays, this assay is free of interference by all steroids tested. Although LC-MS/MS has been used previously for the measurement of plasma, serum and urinary cortisol and cortisone [7][8][9][10] and for salivary cortisol [12,18], to the best of our knowledge this is the first report of a combined LC-MS/MS assay for salivary cortisol and cortisone.…”

Section: Discussionmentioning

confidence: 98%

“…Liquid chromatography-tandem mass spectrometry (LC-MS/MS) can provide the specificity required to eliminate interference by steroids related to cortisol and has been used previously for the combined quantification of cortisol and cortisone in serum, plasma and urine but not saliva [7][8][9][10]. We aimed to develop an LC-MS/MS assay for the simultaneous measurement of cortisol and cortisone in saliva, with the intention of understanding the factors regulating salivary cortisol concentration and as a tool to study the cortisol-cortisone shuttle.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography–tandem mass spectrometry: Application in basal and stimulated conditions

Perogamvros

Owen²,

Newell‐Price

et al. 2009

Journal of Chromatography B

131

107

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography–tandem mass spectrometry: Application in basal and stimulated conditions

Perogamvros

Owen²,

Newell‐Price

et al. 2009

Journal of Chromatography B

131

107

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“…Serum cortisol and cortisone were determined by liquid chromatography mass spectrometry according to Ionita et al (27). Plasma ACTH was analyzed by a chemiluminescent immunometric assay (Immulite 2000, Siemens AG, Munich, Germany).…”

Section: Biochemical Analysismentioning

confidence: 99%

Grapefruit juice and licorice increase cortisol availability in patients with Addison's disease

Methlie

Husebye

Hustad

et al. 2011

European Journal of Endocrinology

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Objective: Failure to mirror the diurnal cortisol profile could contribute to the impaired subjective health status in Addison's disease (AD). Some patients report benefit from the use of various nutritional compounds. The objective of this study was to investigate the impact of licorice and grapefruit juice (GFJ) on the absorption and metabolism of cortisone acetate (CA). Design: Patients (nZ17) with AD on stable CA replacement therapy were recruited from the outpatient clinic at Haukeland University Hospital, Norway. They were assessed on their ordinary CA medication and following two 3-day periods of co-administration of licorice or GFJ. Methods: Time series of glucocorticoids (GCs) in serum and saliva were obtained, and GCs in 24 h urine samples were determined. The main outcome measure was the area under the curve (AUC) for serum cortisol in the first 2.6 h after orally administered CA. Results: Compared with the ordinary treatment, the median AUC for serum cortisol increased with licorice (53 783 vs 50 882, P!0.05) and GFJ (60 661 vs 50 882, P!0.05). Median cortisol levels in serum were also elevated 2.6 h after tablet ingestion (licorice 223 vs 186 nmol/l, P!0.05; GFJ 337 vs 186 nmol/l, P!0.01). Licorice increased the median urinary cortisol/cortisone ratio (0.43 vs 0.21, P!0.00001), whereas GFJ increased the (allo-tetrahydrocortisolCtetrahydrocortisol)/tetrahydrocortisone ratio (0.55 vs 0.43, P!0.05).

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“…This HPLC method has a chromatography time of 32 min, requires a sample volume of 1 mL and has a limit of quantitation of 10 nmol/L for all analytes. Numerous liquid chromatography tandem mass spectrometry methods for urine free cortisol [14,15] and cortisone [16], plasma cortisol and cortisone [17], salivary cortisol and cortisone [10] and synthetic glucocorticoids [18,19] have been described, some of which are cited here.…”

Section: Abbreviationsmentioning

confidence: 99%

Measurement of cortisol, cortisone, prednisolone, dexamethasone and 11-deoxycortisol with ultra high performance liquid chromatography–tandem mass spectrometry: Application for plasma, plasma ultrafiltrate, urine and saliva in a routine laboratory

McWhinney

Briscoe

Ungerer

et al. 2010

Journal of Chromatography B

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Development of a sensitive and selective method for the quantitative analysis of cortisol, cortisone, prednisolone and prednisone in human plasma

Cited by 71 publications

References 24 publications

Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography–tandem mass spectrometry: Application in basal and stimulated conditions

Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography–tandem mass spectrometry: Application in basal and stimulated conditions

Grapefruit juice and licorice increase cortisol availability in patients with Addison's disease

Measurement of cortisol, cortisone, prednisolone, dexamethasone and 11-deoxycortisol with ultra high performance liquid chromatography–tandem mass spectrometry: Application for plasma, plasma ultrafiltrate, urine and saliva in a routine laboratory

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