Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography–tandem mass spectrometry: Application in basal and stimulated conditions

Perogamvros, Ilias; Owen, Laura; Newell‐Price, John; Ray, David; Trainer, Peter J; Keevil, Brian

doi:10.1016/j.jchromb.2009.09.014

Cited by 131 publications

(120 citation statements)

References 19 publications

(22 reference statements)

Supporting

Mentioning

110

Contrasting

Unclassified

Order By: Relevance

“…2A). The concentrations of salivary cortisone were about 4 times higher than cortisol at 6:00-7:00, a.m., 7 times higher at 1:00-2:00, p.m. and 9 times higher at 4:30-5:30, p.m. During the daytime (6:00, a.m.-5:30, p.m.), typical diurnal rhythms of salivary cortisol and cortisone were observed, in agreement with previously reported results in healthy adults (19,25,26). The cortisol/cortisone ratio was also highest at 6:00-7:00, a.m. and then the ratio gradually de-http://bmbreports.org Table 1.…”

Section: Saliva Sample Measurements In Healthy Volunteerssupporting

confidence: 92%

“…Intra and inter-run accuracy, precision, limits of quantitation (LOQ) and recovery of the LC-MS/MS method for the simultaneous analysis of saliva cortisol and cortisone Concentration (ng/ml) Intra-assay (n = 5) Inter-assay (n = 5) Recovery ± SEM a (%) LOQ (ng/ml) Accuracy (%) CV (%) Accuracy (%) CV (%) (Table 2). In basal condition, the averaged ratios from different collection times showed a similar pattern to the ratios measured by LC-MS/MS in English adults (19). The concentrations of cortisol in saliva were also compared with those of cortisone (Fig.…”

Section: Saliva Sample Measurements In Healthy Volunteerssupporting

confidence: 55%

“…The curve showed a tendency towards a plateau at higher concentration levels, probably implicating the saturation of the 11β-HSD type 2 at high concetrations of the substrate (19). These results show the status of cortisol-cortisone shuttle in saliva during the daytime and also give evidence for the activity of 11β-HSD type 2, that catalyzes the irreversible conversion of hormonally active cortisol to inactive cortisone (19,25). In this study, we validated a fast, sensitive and robust LC-MS/MS assay for simultaneous measurement of cortisol and cortisone in saliva and evaluated the concentration levels of salivary cortisol and cortisone in Korean adults.…”

Section: Saliva Sample Measurements In Healthy Volunteersmentioning

confidence: 69%

“…Evaluation of salivary free cortisol has been found to be a good indicator of unbound concentrations of cortisol in serum or plasma (15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

“…LC-MS/MS also provides the specificity needed to overcome interference by similar steroids. The salivary levels of cortisol and cortisone in an English population were recently reported using LC-MS/MS (19).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

Lee¹,

Kwon

Shin

et al. 2010

BMB Reports

View full text Add to dashboard Cite

The levels of salivary cortisol and cortisone in Korean adults were measured for the first time using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The salivary cortisol and cortisone were separated within 10 min. The regression coefficients (r) of the calibration curves were greater than 0.999 for the two steroids. The limits of quantitation (LOQ) were 0.2 ng/ml for cortisol and 1 ng/ml for cortisone. The intra-day precisions of the assay were ＜3.9% and 8.6% for cortisol and cortisone respectively, and the inter-day precisions were ＜1.9% and 4.3% for cortisol and cortisone, respectively. The salivary cortisone concentrations were approximately 4-9 times higher than those of salivary cortisol during the daytime. Diurnal rhythms, during which the cortisol and cortisone concentrations were higher in the morning than in the afternoon, were also observed. The present assay may be useful for the diagnosis of several adrenal dysfunctions in clinical biochemistry. [BMB reports 2010; 43(7): 506-511]

show abstract

Section: Saliva Sample Measurements In Healthy Volunteerssupporting

confidence: 92%

Section: Saliva Sample Measurements In Healthy Volunteerssupporting

confidence: 55%

Section: Saliva Sample Measurements In Healthy Volunteersmentioning

confidence: 69%

“…Evaluation of salivary free cortisol has been found to be a good indicator of unbound concentrations of cortisol in serum or plasma (15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

Lee¹,

Kwon

Shin

et al. 2010

BMB Reports

View full text Add to dashboard Cite

show abstract

LC‐MS Bioanalysis of Steroids

Zhang¹,

Stanczyk

2013

Handbook of LC‐MS Bioanalysis

View full text Add to dashboard Cite

The association between the acute psychobiological stress response in second trimester pregnant women, amniotic fluid glucocorticoids, and neonatal birth outcome

Ghaemmaghami

Dainese

Marca

et al. 2013

Developmental Psychobiology

View full text Add to dashboard Cite

The underlying biological mechanism of prenatal stress in humans is poorly understood, but maternal cortisol (F) excess seems to play an important role. In pregnant rats, acute stress causes an up-regulation of placental 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), an enzyme present throughout the body (e.g., placenta, salivary glands) that inactivates F to cortisone (E), thereby protecting the fetus from maternal F overexposure. Whether acute stress influences human 11β-HSD2 is unclear. We aimed to explore the association between the maternal stress reactivity and amniotic fluid F, E, and the E/(E + F) ratio as a marker of fetoplacental 11β-HSD2. The predictive value of all markers for birth outcome was investigated. We examined 34 healthy pregnant women undergoing amniocentesis, which served as a standardized, real-life stressor. F, E, and E/(E + F) were determined from a single aliquot of amniotic fluid, and from saliva samples collected repeatedly. Subjects filled out state questionnaires repeatedly and were re-examined in a control condition after notification of a normal amniocentesis result. During amniocentesis, psychological stress, salivary F (SalF), and salivary E (SalE) increased significantly, whereas SalE/(E + F) decreased. The SalF reactivity was positively associated with amniotic E, while SalE/(E + F) was inversely associated with amniotic E/(E + F). SalF and SalE predicted lower and SalE/(E + F) higher birth weight. Psychological and amniotic fluid variables were unrelated to birth outcome. Findings indicate that maternal F is inactivated to E in the human fetoplacental unit during acute stress. Increased 11β-HSD2 activity within the maternal salivary glands following acute stress may mirror further stress protective mechanisms worthwhile investigating.

show abstract

Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography–tandem mass spectrometry: Application in basal and stimulated conditions

Cited by 131 publications

References 19 publications

Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

LC‐MS Bioanalysis of Steroids

The association between the acute psychobiological stress response in second trimester pregnant women, amniotic fluid glucocorticoids, and neonatal birth outcome

Contact Info

Product

Resources

About