Development of a sensitive and specific qPCR method to detect duck and goose DNA in meat and feathers

Natonek-Wiśniewska, Małgorzata; Krzyscin, P.; Bugno‐Poniewierska, Monika

doi:10.1007/s00217-018-3165-6

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…In practice, DNA can be identified regardless of matrix form or earlier processing. We can freely determine species composition of both raw tissues and processed tissues in the form of meat, bones, blood, eggs, dairy products such as cheese, milk and butter, drinks, gelatin, lyophilized milk products, meat preparations, and egg products [7,12,[27][28][29].…”

Section: The Scope Of the Species Identification Testsmentioning

confidence: 99%

Detection of the Species Composition of Food Using Mitochondrial DNA: Challenges and Possibilities of a Modern Laboratory

Natonek-Wiśniewska¹,

Krzyscin²

2020

Biochemical Analysis Tools - Methods for Bio-Molecules Studies

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Monitoring food quality is an important and constant element of the food market. This need is connected with health issues, religious beliefs of consumers, and economic considerations. For analysis, mtDNA is most commonly used because it is resist to physical factors such as temperature and pressure, which very often accompany food processing. Nowadays, scientific publications present a number of methods describing species identification from both farm animals and also less common animals. The most effective methods for determining species are based on PCR, real-time PCR, and sequencing. The methods are very sensitive, limit of detection (LOD) is 0.001% for many of them. An indispensable element of performing the described research is the strict application in the laboratory of several principles, which are intended to improve the work and make it safe for the lab technician, as well as guarantee the quality and effectiveness of the experiments carried out. The high work requirements set for the crew naturally shape the quality system from which the most popular is ISO/IEC 17025. Modern methods based on mtDNA are a good tool for food analysis, creating great opportunities for the researcher, at the same time causing challenges for the contemporary laboratory.

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Section: The Scope Of the Species Identification Testsmentioning

confidence: 99%

Detection of the Species Composition of Food Using Mitochondrial DNA: Challenges and Possibilities of a Modern Laboratory

Natonek-Wiśniewska¹,

Krzyscin²

2020

Biochemical Analysis Tools - Methods for Bio-Molecules Studies

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“…Since it is simple, rapid, and cost-effective in contrast to DNA sequencing, it is also suitable 1 for species identification. Various methods using a species-specific qPCR assay have been reported for the identification of vertebrates such as fish (Terio et al, 2010;Hird et al, 2012;Hulley, Tharmalingam, Zarnke, & Boreham, 2019;Watanabe, Minegishi, Yoshinaga, Aoyama, & Tsukamoto, 2004;Taylor, Fox, Rico, & Rico, 2002), birds (Rojas et al, 2010;Natonek-Wiśniewska, Krzyścin, & Bugno-Poniewierska, 2019;Kesmen, Yetiman, Sahin, & Yetim, 2012), and mammals (Ji, Bai, Ji, & Yang, 2011;Wozney & Wilson, 2012;O'Neill et al, 2013;Knapp, Umhang, Poulle, & Millon, 2016). Probe-based qPCR assays are especially sensitive and specific, and can detect even tiny amounts of target species DNA with high reliability.…”

Section: Introductionmentioning

confidence: 99%

A vertebrate-specific qPCR assay as an endogenous internal control for robust species identification

Taniguchi,

Akutsu,

Watanabe

et al. 2024

Preprint

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The identification of vertebrate species is important in numerous fields such as ecology, archaeology, and food and forensic sciences. Real-time quantitative PCR (qPCR) assays specific for one vertebrate species are promising approaches for species identification, although there are several drawbacks such as difficulty determining whether the detected DNA is authentic or a contaminant. Here, we describe a qPCR assay specific for vertebrate mitochondrial DNA (mtDNA) which can overcome these drawbacks. Since we found that mitochondrial 16S rRNA contains regions that are perfectly (not highly) conserved across virtually all vertebrates, but are variable in invertebrates, we were able to design a vertebrate-specific qPCR assay by placing primers/probe within these regions. The specificity and accuracy of this assay were validated with representative vertebrate and invertebrate samples. This assay detected DNA from all vertebrate samples, but not from any invertebrate samples. In addition, this assay was able to quantify vertebrate mtDNAs as accurately as previously reported species-specific qPCR assays. The results demonstrated it is feasible to quantify vertebrate mtDNA specifically and accurately in a sample. This means that it is possible to determine the ratio of specific vertebrate species mtDNA to total vertebrate mtDNA in a sample. In conjunction with this assay as an endogenous internal control, species-specific qPCR assays will allow for the robust identification of vertebrate species.

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“…Data may be preliminary. & Boreham, 2019;Watanabe, Minegishi, Yoshinaga, Aoyama, & Tsukamoto, 2004;Taylor, Fox, Rico, & Rico, 2002), birds (Rojas et al, 2010;Natonek-Wiśniewska, Krzyścin, & Bugno-Poniewierska, 2019;Kesmen, Yetiman, Sahin, & Yetim, 2012), and mammals (Ji, Bai, Ji, & Yang, 2011;Wozney & Wilson, 2012;O'Neill et al, 2013;Knapp, Umhang, Poulle, & Millon, 2016). Probe-based qPCR assays are especially sensitive and specific, and can detect even tiny amounts of target species DNA with high reliability.…”

Section: Introductionmentioning

confidence: 99%

A vertebrate-specific qPCR assay for species identification

Taniguchi,

Akutsu,

Watanabe

et al. 2024

Preprint

View full text Add to dashboard Cite

The identification of vertebrate species is important in numerous fields such as ecology, archaeology, and food and forensic sciences. Real-time quantitative PCR (qPCR) assays specific for one vertebrate species are promising approaches for species identification, although there are several drawbacks such as difficulty determining whether the detected DNA is authentic or a contaminant. Here, we describe a qPCR assay specific for vertebrate mitochondrial DNA (mtDNA) which can overcome these drawbacks. Since we found that mitochondrial 16S rRNA contains regions that are perfectly (not highly) conserved across virtually all vertebrates, but are variable in invertebrates, we were able to design a vertebrate-specific qPCR assay by placing primers/probe within these regions. The specificity and accuracy of this assay were validated with representative vertebrate and invertebrate samples. This assay detected DNA from all vertebrate samples, but not from any invertebrate samples. In addition, this assay was able to quantify vertebrate mtDNAs as accurately as previously reported species-specific qPCR assays. The results demonstrated it is feasible to quantify vertebrate mtDNA specifically and accurately in a sample. In conjunction with this assay as an endogenous internal control, species-specific qPCR assays will allow for the robust identification of vertebrate species.

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Development of a sensitive and specific qPCR method to detect duck and goose DNA in meat and feathers

Cited by 6 publications

References 12 publications

Detection of the Species Composition of Food Using Mitochondrial DNA: Challenges and Possibilities of a Modern Laboratory

Detection of the Species Composition of Food Using Mitochondrial DNA: Challenges and Possibilities of a Modern Laboratory

A vertebrate-specific qPCR assay as an endogenous internal control for robust species identification

A vertebrate-specific qPCR assay for species identification

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