Development of a sensitive monoclonal antibody-based ELISA for the determination of a β-adrenergic agonist brombuterol in swine meat, liver and feed samples

Abstract

A sensitive enzyme-linked immunosorbent assay (ELISA) based on a newly prepared monoclonal antibody (mAb) for the determination of a β-adrenergic agonist brombuterol in swine meat, liver and feed samples was developed.

A Fast Method for the Simultaneous Analysis of 26 Beta-Agonists in Swine Muscle with a Multi-Functional Filter by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

“…The qualified rates for beta-agonist residues in China remained lower than the satisfactory level, which is higher than 98%. In addition, many research institutions and universities in China analyzed beta-agonist residues in swine muscle samples collected from local supermarkets, traditional markets, and slaughterhouses [8][9][10][11] and confirmed that clenbuterol, salbutamol, and ractopamine were detected in a few meat samples. Therefore, the strict monitoring and control of beta-agonist residues are still required to meet the regulations in different countries.…”

Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles

“…However, the instruments are expensive, and their operation is complicated and requires professional personnel; moreover, these approaches cannot meet the need for rapid, large-scale sample screening and are therefore limited in practical applications. 12 Immunoassays mainly include the enzyme-linked immunosorbent assay (ELISA), 13 , 14 chemiluminescence immunoassay (CLIA) 15 and immunochromatography (ICA). 16 , 17 ELISA has the advantages of high sensitivity and high throughput, but it also presents the disadvantages of a high false positive rate and inability to provide rapid on-site detection.…”

A novel colorimetric immunosensor based on platinum colloid nanoparticles immobilized on PowerVision as signal probes and Fe3O4@β‐cyclodextrin as capture probes for ractopamine detection in pork

“…In recent years, the enzyme-linked immunosorbent assay (ELISA) has attracted increasing attention for rapid screening as a result of its low cost, simple manipulation and short assay time. [11][12][13][14] Although the traditional ELISA for RAC detection using competitive methods is fast and effective, it still has a high detection limit and produces many false positives. To overcome these drawbacks, we propose a novel immunosensor based on ELISA for the sensitive, low-cost and rapid detection of RAC on-site.…”