This article describes in-flight measurements to delay laminar-turbulent transition by means of active Tollmien-Schlichting (TS) wave cancellation. The damping of unstable TS waves in the boundary layer leads to downstream shifting of the laminar-turbulent transition and therefore to the reduction of skin friction. In-flight experiments were carried out using a laminar wing glove for a sailplane. A sensor-actuator system attached to the wing glove consisted of an array of surface hot-wire reference sensors to detect oncoming TS-waves upstream of a membrane actuator and surface hot-wire error sensors downstream of the actuator. The method applied to delay laminar-turbulent transition is based on damping of naturally occurring instabilities through superimposition of the counter wave, which is calculated by a fast digital signal processor using a closed-loop feed-forward control algorithm. The experiments were carried out at flight velocities in the region of 20 m/s, which corresponds to a chord Reynolds number of about 2 million. The results show a damping of ∼50 per cent reduction of the local amplitudes of the instabilities. It is anticipated that using an actuator with a high resonance frequency and a minimal reaction time will improve damping significantly in future experiments.