Development of a sequential injection analysis system for the simultaneous biosensing of glucose and ethanol in bioreactor fermentation

Lapa, Rui A. S.; Lima, José L.F.C.; Pinto, Ivone V. O. S.

doi:10.1016/s0308-8146(02)00395-3

Cited by 39 publications

(18 citation statements)

References 14 publications

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“…3), what is 1.5-5 times higher than published flow enzyme biosensor systems for ethanol fermentation monitoring [18][19][20]. The significant improvement of linear range is combined effect of use of Gluconobacter periplasmatic membranebound PQQ-dependent alcohol dehydrogenase (PQQ-ADH) instead of NAD + -dependent ADH, and use of described FIA set-up.…”

Section: Characteristics Of Biosensormentioning

confidence: 97%

Ethanol Gluconobacter biosensor designed for flow injection analysis

Valach

Katrlı́k

Šturdı́k

et al. 2009

Sensors and Actuators B: Chemical

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Section: Characteristics Of Biosensormentioning

confidence: 97%

Ethanol Gluconobacter biosensor designed for flow injection analysis

Valach

Katrlı́k

Šturdı́k

et al. 2009

Sensors and Actuators B: Chemical

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“…When monitoring parameters in fermentation broths, it is however important to reduce both residence time in the loop and the ratio of loop volume to working volume in the bioreactor to prevent any effect on the performance of the microbial culture in terms of substrate availability due to the recirculation process (10). It should be borne in mind that yields of flow-through dialysis are frequently below 15% wherefore dialysis modules/probes or filter probes have been tailored successfully to bioreactors for in-line dilution of target species (11,12).…”

Section: X2 Fundamentals Of Flow Injection Analysismentioning

confidence: 99%

“…And since all manipulations are computer controlled, it is easy and simple to reprogram the system from one application to another one, providing great versatility for monitoring multiple variables in fermentation broths (12,37) since all operating protocols such as sample injection, mixing, and separation are controlled precisely by the software without need for physical reconfiguration of the system. However, it is generally difficult to accommodate (stack) more than two reagents along with the sample, although additional reagents might be added further downstream, i.e., by making an FI/SI-hybrid.…”

Section: X5 Fundamentals Of Sequential Injection Analysismentioning

confidence: 99%

Flow Injection Analysis in Industrial Biotechnology

Hansen

Miró

2009

Encyclopedia of Industrial Biotechnology

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Flow injection analysis (FIA) is an analytical chemical continuous flow (CF) method which, in contrast to traditional CF procedures, does not rely on complete physical mixing (homogenization) of the sample and the reagent(s) or on attaining chemical equilibria of the chemical reactions involved. Exploiting controllable dispersion of the injected sample within the reagent‐containing carrier stream and strictly reproducible timing of all events taking place, it is based on measuring transient signals, which not only implies very high sampling rates but also, and most importantly, permits implementation of a number of novel methodologies that are not feasible when performed under batch conditions or by conventional CF procedures. Demonstrated for selected bioanalytical and technological applications encompassing cellular and enzymatic assays as well as monitoring of culture media, the principles and operational characteristics of FIA are first outlined, and then its downscaled/miniaturized sequels, that is, sequential injection analysis (SIA) and lab‐on‐valve (LOV), are detailed. Thus, in SIA the sample and reagents are, via the use of a multi‐position valve and an attached syringe pump operated under full programmable control, aspirated sequentially and then propelled forward allowing the sample and reagent(s) to be intermixed and, if called for, subjected to appropriate treatments before analyte detection. This infers that, merely minute sample/reagent volumes are consumed, hence leading to generation of small amounts of waste. In LOV this downscaling is taken further by using a small monolithic structure within which all sample manipulations and ultimate analyte detection under programmable control can be effected. Even bead materials with different surface groups/characteristics, including live cells, can be handled and utilized as demonstrated. Because the syringe pump in SIA and LOV can be used for accurately aspirating, propelling or even stopping the flow, these modi operandi allow fully to exploit the interplay between the kinetics and the thermodynamics of the chemical reactions involved, so that there are no restrictions whatsoever as to the chemistries that can be implemented, even if they entail multistep reactions. Representative bioanalytical examples of this interplay are presented.

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“…For simple and rapid measurement, enzyme biosensors for ethanol have been developed using ALDH (Kitagawa et al, 1991;Niculescu, Mieliauskiene, Laurinavicius, & Csoregi, 2003) or alcohol oxidase (Azevedo, Prazeres, Cabral, & Fonseca, 2005;Huseyin & Levent, 2006;Lapa, Lima, & Pinto, 2003). However, both enzymes lack stability, and purification is expensive.…”

Section: Introductionmentioning

confidence: 99%