Development of a serum-free culture medium for the large scale production of recombinant protein from a Chinese hamster ovary cell line

Keen, Michael; Rapson, N. T.

doi:10.1007/bf00749653

Cited by 79 publications

(41 citation statements)

References 18 publications

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“…As a result, traditional basal media from which serum is excluded were sometimes unable to adequately support cell growth 94. For example, Barnes et al.…”

Section: History Of Cell Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

394

278

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BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

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“…As a result, traditional basal media from which serum is excluded were sometimes unable to adequately support cell growth 94. For example, Barnes et al.…”

Section: History Of Cell Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

394

278

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“…However, bovine insulin can easily be replaced by recombinant human insulin (Keen and Rapson, 1995).…”

Section: Adaptation Of Cho Dukxb11 and Recombinant Cell Lines To Serumentioning

confidence: 99%

“…human macrophage colony stimulating factor (Sinacore et al, 1996), human clotting factor IX (Sinacore et al, 1996), γ-interferon Xie et al, 1997), and the humanized antibody CAMPATH-1H (Keen and Rapson, 1995). However, the increase in productivity under serumsupplemented conditions was offset by lower cell densities of confluent cultures in the presence of FCS (usually around 4·10 4 cells/cm 2 ).…”

Section: Production Of Atiii Under Serum-free Conditionsmentioning

confidence: 99%

Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11

Schröder

Matischak

Friedl

2004

Journal of Biotechnology

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Citation for published item:hr¤ oderD wF nd w tis h kD uF nd priedlD F @PHHRA 9 erumE nd proteinEfree medi formul tions for the ghinese h mster ov ry ell line h u fIIF9D tourn l of iote hnologyFD IHV @QAF ppF PUWEPWPF Further information on publisher's website: httpXGGdxFdoiForgGIHFIHITGjFj iote FPHHQFIPFHHS Publisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. AbstractThe production of therapeutic proteins in mammalian cell lines is of outstanding importance. The maintenance of most mammalian cell lines in culture requires the addition of serum to the culture medium.The elimination of serum from mammalian cell culture is desirable since serum is expensive and a source of contaminants, e.g. viruses, mycoplasma or prions. Here we describe the composition of serum-and protein-free media for the Chinese hamster ovary (CHO) cell line DUKXB11. The serum-free formulation supports excellent growth of CHO DUKXB11 cells at low (23 cells/cm 2 ) and high (2·10 4 cells/cm 2 ) seeding densities characterized by a generation time of 10 -12 h, and, after addition of 0.2% pluronic F-68, the growth of a recombinant suspension cell line derived from DUKXB11. In addition, this formulation also allowed us to adapt recombinant cell lines expressing various amounts of human antithrombin ATIII (ATIII)to serum-free conditions. Secretion of ATIII was readily observed in the serum-free medium. Minor changes to the serum-free formulation resulted in a protein free formulation that supported growth of CHO DUKXB11 cells, growth of recombinant CHO cells expressing ATIII, and production of ATIII. Key wordsChinese hamster ovary cells, serum-free medium, protein-free medium, recombinant protein production,

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“…Recently, there has been researches using cells, including embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, for pharmaceutical and regenerative medical applications. Several metals are also considered essential components in media as well as the human body [16,17]. Eagle's minimal essential medium (EMEM) is one of the most traditional and widely used synthetic medium containing 5 to 10% serum [18].…”

Section: Introductionmentioning

confidence: 99%

Speciation of Intracellular Zn, Fe and Cu within both iPS Cells and Differentiated Cells Using HPLC Coupled to ICP-MS

2016

J Anal Bioanal Tech

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Zinc, iron and copper are essential mineral nutrients for the human body to function. It is also known that there are biological processes which relate to metals in the body including cells. Therefore, understanding about intracellular metals is important for the regeneration medicine. Intracellular metals were thought to be able to be divided into two forms by function: i) protein binding metals and ii) labile metals. However, it is difficult to quantify the concentration in both intracellular metal forms. In this study, the analytical method including the pre-treatment and HPLC coupled to ICP-MS (HPLC-ICP-MS) techniques was developed. Our method is able to fractionate two forms of intracellular metals and quantify each concentration, simultaneously. Finally, the presented method was applied to analysis of several cell states. As a result, dynamic changes of intracellular metal concentration were demonstrated during cell differentiation from iPS cells to pancreatic β cells.

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Development of a serum-free culture medium for the large scale production of recombinant protein from a Chinese hamster ovary cell line

Cited by 79 publications

References 18 publications

Animal‐cell culture media: History, characteristics, and current issues

Animal‐cell culture media: History, characteristics, and current issues

Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11

Speciation of Intracellular Zn, Fe and Cu within both iPS Cells and Differentiated Cells Using HPLC Coupled to ICP-MS

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